Abstract
Bursal epithelial cells derived from 14-day-old chick embryos were cultured under a serum-free condition and the recovered culture supernatant was analyzed for surface immunoglobulin M (sIgM)-inducing activity on the bursacytes of 12-day-old chick embryos. Surface IgM positive rate of bursacytes incubated with the culture supernatant increased significantly, thus indicating the presence of sIgM-inducing factor(s) in the culture supernatant. The sIgM-inducing factor(s) in the supernatant was analyzed by gel filtration chromatography and HPLC. Results from these analyses indicated that the factor may be a polypeptide with a molecular weight of about 1300. This substance which is different from already known factors, such as Bursin, may be a newly discovered B cell differentiating factor in the bursa of Fabricius that promotes chicken B cell differentiation.
| Original language | English |
|---|---|
| Pages (from-to) | 130-138 |
| Number of pages | 9 |
| Journal | Journal of Poultry Science |
| Volume | 40 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2003 |
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Keywords
- bursa of Fabricius
- epithelial cells
- sIgM-inducing factor
ASJC Scopus subject areas
- Animal Science and Zoology
Cite this
Surface IgM-Inducing Factor in the Culture Supernatant of Bursal Epithelial Cells Derived from Chick Embryos. / Han, Song; Han, Song; Kondo, Mineyuki; Abe, Asaki; Kimura, Yoshinobu; Ohtsuki, Kazumasa; Kondo, Yasuhiro.
In: Journal of Poultry Science, Vol. 40, No. 2, 2003, p. 130-138.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Surface IgM-Inducing Factor in the Culture Supernatant of Bursal Epithelial Cells Derived from Chick Embryos
AU - Han, Song
AU - Han, Song
AU - Kondo, Mineyuki
AU - Abe, Asaki
AU - Kimura, Yoshinobu
AU - Ohtsuki, Kazumasa
AU - Kondo, Yasuhiro
PY - 2003
Y1 - 2003
N2 - Bursal epithelial cells derived from 14-day-old chick embryos were cultured under a serum-free condition and the recovered culture supernatant was analyzed for surface immunoglobulin M (sIgM)-inducing activity on the bursacytes of 12-day-old chick embryos. Surface IgM positive rate of bursacytes incubated with the culture supernatant increased significantly, thus indicating the presence of sIgM-inducing factor(s) in the culture supernatant. The sIgM-inducing factor(s) in the supernatant was analyzed by gel filtration chromatography and HPLC. Results from these analyses indicated that the factor may be a polypeptide with a molecular weight of about 1300. This substance which is different from already known factors, such as Bursin, may be a newly discovered B cell differentiating factor in the bursa of Fabricius that promotes chicken B cell differentiation.
AB - Bursal epithelial cells derived from 14-day-old chick embryos were cultured under a serum-free condition and the recovered culture supernatant was analyzed for surface immunoglobulin M (sIgM)-inducing activity on the bursacytes of 12-day-old chick embryos. Surface IgM positive rate of bursacytes incubated with the culture supernatant increased significantly, thus indicating the presence of sIgM-inducing factor(s) in the culture supernatant. The sIgM-inducing factor(s) in the supernatant was analyzed by gel filtration chromatography and HPLC. Results from these analyses indicated that the factor may be a polypeptide with a molecular weight of about 1300. This substance which is different from already known factors, such as Bursin, may be a newly discovered B cell differentiating factor in the bursa of Fabricius that promotes chicken B cell differentiation.
KW - bursa of Fabricius
KW - epithelial cells
KW - sIgM-inducing factor
UR - http://www.scopus.com/inward/record.url?scp=22544435106&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=22544435106&partnerID=8YFLogxK
U2 - 10.2141/jpsa.40.130
DO - 10.2141/jpsa.40.130
M3 - Article
AN - SCOPUS:22544435106
VL - 40
SP - 130
EP - 138
JO - Journal of Poultry Science
JF - Journal of Poultry Science
SN - 1346-7395
IS - 2
ER -