Abstract
Bursal epithelial cells derived from 14-day-old chick embryos were cultured under a serum-free condition and the recovered culture supernatant was analyzed for surface immunoglobulin M (sIgM)-inducing activity on the bursacytes of 12-day-old chick embryos. Surface IgM positive rate of bursacytes incubated with the culture supernatant increased significantly, thus indicating the presence of sIgM-inducing factor(s) in the culture supernatant. The sIgM-inducing factor(s) in the supernatant was analyzed by gel filtration chromatography and HPLC. Results from these analyses indicated that the factor may be a polypeptide with a molecular weight of about 1300. This substance which is different from already known factors, such as Bursin, may be a newly discovered B cell differentiating factor in the bursa of Fabricius that promotes chicken B cell differentiation.
Original language | English |
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Pages (from-to) | 130-138 |
Number of pages | 9 |
Journal | Journal of Poultry Science |
Volume | 40 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2003 |
Keywords
- bursa of Fabricius
- epithelial cells
- sIgM-inducing factor
ASJC Scopus subject areas
- Animal Science and Zoology