Surface IgM-Inducing Factor in the Culture Supernatant of Bursal Epithelial Cells Derived from Chick Embryos

Song Han, Song Han, Mineyuki Kondo, Asaki Abe, Yoshinobu Kimura, Kazumasa Ohtsuki, Yasuhiro Kondo

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Bursal epithelial cells derived from 14-day-old chick embryos were cultured under a serum-free condition and the recovered culture supernatant was analyzed for surface immunoglobulin M (sIgM)-inducing activity on the bursacytes of 12-day-old chick embryos. Surface IgM positive rate of bursacytes incubated with the culture supernatant increased significantly, thus indicating the presence of sIgM-inducing factor(s) in the culture supernatant. The sIgM-inducing factor(s) in the supernatant was analyzed by gel filtration chromatography and HPLC. Results from these analyses indicated that the factor may be a polypeptide with a molecular weight of about 1300. This substance which is different from already known factors, such as Bursin, may be a newly discovered B cell differentiating factor in the bursa of Fabricius that promotes chicken B cell differentiation.

Original languageEnglish
Pages (from-to)130-138
Number of pages9
JournalJournal of Poultry Science
Volume40
Issue number2
DOIs
Publication statusPublished - 2003

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immunoglobulin M
embryo (animal)
epithelial cells
chicks
B-lymphocytes
bursa of Fabricius
cell differentiation
polypeptides
high performance liquid chromatography
molecular weight
chickens

Keywords

  • bursa of Fabricius
  • epithelial cells
  • sIgM-inducing factor

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Surface IgM-Inducing Factor in the Culture Supernatant of Bursal Epithelial Cells Derived from Chick Embryos. / Han, Song; Han, Song; Kondo, Mineyuki; Abe, Asaki; Kimura, Yoshinobu; Ohtsuki, Kazumasa; Kondo, Yasuhiro.

In: Journal of Poultry Science, Vol. 40, No. 2, 2003, p. 130-138.

Research output: Contribution to journalArticle

Han, Song ; Han, Song ; Kondo, Mineyuki ; Abe, Asaki ; Kimura, Yoshinobu ; Ohtsuki, Kazumasa ; Kondo, Yasuhiro. / Surface IgM-Inducing Factor in the Culture Supernatant of Bursal Epithelial Cells Derived from Chick Embryos. In: Journal of Poultry Science. 2003 ; Vol. 40, No. 2. pp. 130-138.
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