Suprastructures and dynamic properties of Mycobacterium tuberculosis FtsZ

David Popp, Mitsusada Iwasa, Harold P. Erickson, Akihiro Narita, Yuichiro Maéda, Robert C. Robinson

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Tuberculosis causes the most death in humans by any bacterium. Drug targeting of bacterial cytoskeletal proteins requires detailed knowledge of the various filamentous suprastructures and dynamic properties. Here, we have investigated by high resolution electron microscopy the assembly of cell division protein and microtubule homolog FtsZ from Mycobacterium tuberculosis (MtbFtsZ) in vitro in the presence of various monovalent salts, crowding agents and polycations. Supramolecular structures, including two-dimensional rings, three-dimensional toroids, and multistranded helices formed in the presence of molecular crowding, were similar to those observed by fluorescence microscopy in bacteria in vivo. Dynamic properties of MtbFtsZ filaments were visualized by light scattering and real time total internal reflection fluorescence microscopy. Interestingly, MtbFtsZ revealed a form of dynamic instability at steady state. Cation-induced condensation phenomena of bacterial cytomotive polymers have not been investigated in any detail, although it is known that many bacteria can contain high amounts of polycations, which may modulate the prokaryotic cytoskeleton. We find that above a threshold concentration of polycations which varied with the valence of the cation, ionic strength, and pH, MtbFtsZ mainly formed sheets. The general features of these cation-induced condensation phenomena could be explained in the framework of the Manning condensation theory. Chirality and packing defects limited the dimensions of sheets and toroids at steady state as predicted by theoretical models. In first approximation simple physical principles seem to govern the formation of MtbFtsZ suprastructures.

Original languageEnglish
Pages (from-to)11281-11289
Number of pages9
JournalJournal of Biological Chemistry
Volume285
Issue number15
DOIs
Publication statusPublished - Apr 9 2010
Externally publishedYes

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Radiation Scattering
Bacteriology
pH
Biophysics
Light reflection
Refractometry
Stereochemistry
Cytoskeletal Proteins
Bacterial Proteins
Fluorescence microscopy
Cell proliferation
Ionic strength
Cytoskeleton
Mycobacterium tuberculosis
Fluorescence Microscopy
Electron Scanning Microscopy
Osmolar Concentration
Electron microscopy
Cations
Condensation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Suprastructures and dynamic properties of Mycobacterium tuberculosis FtsZ. / Popp, David; Iwasa, Mitsusada; Erickson, Harold P.; Narita, Akihiro; Maéda, Yuichiro; Robinson, Robert C.

In: Journal of Biological Chemistry, Vol. 285, No. 15, 09.04.2010, p. 11281-11289.

Research output: Contribution to journalArticle

Popp, David ; Iwasa, Mitsusada ; Erickson, Harold P. ; Narita, Akihiro ; Maéda, Yuichiro ; Robinson, Robert C. / Suprastructures and dynamic properties of Mycobacterium tuberculosis FtsZ. In: Journal of Biological Chemistry. 2010 ; Vol. 285, No. 15. pp. 11281-11289.
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