Suppressive effects of interferons on the production and release of human T-lymphotropic virus type-I (HTLV-I)

Takashi Oka, Y. Ohtsuki, H. Sonobe, M. Furihata, I. Miyoshi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The effects of human α-, β-, or γ-interferon (IFN) on the replication and production of human T-lymphotrophic virus type-I (HTLV-I) were investigated in a human T-cell line, MT-2. Virus transmission and production estimated by syncytium formation and HTLV-I-associated reverse transcriptase (RT) activity were strongly suppressed in the presence of α- and β-IFN, but not γ-IFN. However, the expression of virus specific proteins gp46 but not p19, p24, p28, p36, and gp68 was affected with IFNs as revealed by Western blotting analysis. Electron microscopic observations showed that some of the HTLV-I particles were trapped in the intracellular vacuoles in the presence of high doses of α- or β-IFN. Continuous supply of IFNs appeared to be essential for the constant suppression of RT activity. These results suggest that α- and β-IFN do not inhibit HTLV-I gene expression strikingly but suppress processing or assembly of virus proteins and/or releasing of virions in the late phase of maturation.

Original languageEnglish
Pages (from-to)63-73
Number of pages11
JournalArchives of Virology
Volume115
Issue number1-2
DOIs
Publication statusPublished - Mar 1990
Externally publishedYes

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Interferons
Viruses
RNA-Directed DNA Polymerase
Virion
Virus Assembly
Giant Cells
Vacuoles
Proteins
Western Blotting
Electrons
T-Lymphocytes
Gene Expression
Cell Line

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Genetics

Cite this

Suppressive effects of interferons on the production and release of human T-lymphotropic virus type-I (HTLV-I). / Oka, Takashi; Ohtsuki, Y.; Sonobe, H.; Furihata, M.; Miyoshi, I.

In: Archives of Virology, Vol. 115, No. 1-2, 03.1990, p. 63-73.

Research output: Contribution to journalArticle

Oka, Takashi ; Ohtsuki, Y. ; Sonobe, H. ; Furihata, M. ; Miyoshi, I. / Suppressive effects of interferons on the production and release of human T-lymphotropic virus type-I (HTLV-I). In: Archives of Virology. 1990 ; Vol. 115, No. 1-2. pp. 63-73.
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