Suppressive effects of human herpesvirus 6 on in vitro colony formation of hematopoietic progenitor cells

Hiroki Isomura, Masao Yamada, Mariko Yoshida, Hiroyuki Tanaka, Tetsuro Kitamura, Megumi Oda, Shiro Nii, Yoshiki Seino

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Human herpesvirus 6 (HHV-6) has been reported to be involved in bone marrow failure after bone marrow transplantation (BMT). To elucidate the role of HHV-6 in the marrow failure, we examined the comparative effect of two variants of HHV-6 (HHV-6A and HHV-6B) and human herpesvirus 7 (HHV-7) on in vitro colony formation of hematopoietic progenitor cells in methylcellulose semi-solid media. Progenitor cells prepared from cord blood mononuclear cells (CBMNCs) were infected with one of these viruses at various multiplicity of infection (MOI), and were subjected to methylcellulose colony assay. Formation of both granulocyte/macrophage (CFU-GM) and erythroid (BFU-E) colonies was MOI-dependently suppressed after infection with the Z29 strain of HHV-6B. Although HHV-6A suppressed the formation of BFU-E colonies as efficiently as HHV-6B, the former did not exhibit significant suppressive effect on the formation of CFU-GM colonies at an MOI 1. HHV-7 had no effect on hematopoietic colony formation at all. Based on frequent positivity of viral DNA in single colonies obtained from HHV-6-infected progenitor cells by polymerase chain reaction and in situ hybridization, direct effects of HHV-6 on the hematopoietic progenitor cells are suggested as the cause of the suppression rather than indirect effects via accessory cells of the bone marrow.

Original languageEnglish
Pages (from-to)406-412
Number of pages7
JournalJournal of Medical Virology
Volume52
Issue number4
DOIs
Publication statusPublished - Aug 1997

Fingerprint

Human Herpesvirus 6
Hematopoietic Stem Cells
Human Herpesvirus 7
Granulocyte-Macrophage Progenitor Cells
Erythroid Precursor Cells
Methylcellulose
Infection
Stem Cells
Bone Marrow
In Vitro Techniques
Viral DNA
Bone Marrow Transplantation
Fetal Blood
Granulocytes
Bone Marrow Cells
In Situ Hybridization
Blood Cells
Macrophages
Viruses

Keywords

  • Bone marrow transplantation
  • Burst forming unit erythroid
  • Colony forming unit granulocyte/macrophage
  • Human herpesvirus 7
  • In situ hybridization
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Virology

Cite this

Suppressive effects of human herpesvirus 6 on in vitro colony formation of hematopoietic progenitor cells. / Isomura, Hiroki; Yamada, Masao; Yoshida, Mariko; Tanaka, Hiroyuki; Kitamura, Tetsuro; Oda, Megumi; Nii, Shiro; Seino, Yoshiki.

In: Journal of Medical Virology, Vol. 52, No. 4, 08.1997, p. 406-412.

Research output: Contribution to journalArticle

Isomura, Hiroki ; Yamada, Masao ; Yoshida, Mariko ; Tanaka, Hiroyuki ; Kitamura, Tetsuro ; Oda, Megumi ; Nii, Shiro ; Seino, Yoshiki. / Suppressive effects of human herpesvirus 6 on in vitro colony formation of hematopoietic progenitor cells. In: Journal of Medical Virology. 1997 ; Vol. 52, No. 4. pp. 406-412.
@article{4cb81156e2604b229815fc4c8067649b,
title = "Suppressive effects of human herpesvirus 6 on in vitro colony formation of hematopoietic progenitor cells",
abstract = "Human herpesvirus 6 (HHV-6) has been reported to be involved in bone marrow failure after bone marrow transplantation (BMT). To elucidate the role of HHV-6 in the marrow failure, we examined the comparative effect of two variants of HHV-6 (HHV-6A and HHV-6B) and human herpesvirus 7 (HHV-7) on in vitro colony formation of hematopoietic progenitor cells in methylcellulose semi-solid media. Progenitor cells prepared from cord blood mononuclear cells (CBMNCs) were infected with one of these viruses at various multiplicity of infection (MOI), and were subjected to methylcellulose colony assay. Formation of both granulocyte/macrophage (CFU-GM) and erythroid (BFU-E) colonies was MOI-dependently suppressed after infection with the Z29 strain of HHV-6B. Although HHV-6A suppressed the formation of BFU-E colonies as efficiently as HHV-6B, the former did not exhibit significant suppressive effect on the formation of CFU-GM colonies at an MOI 1. HHV-7 had no effect on hematopoietic colony formation at all. Based on frequent positivity of viral DNA in single colonies obtained from HHV-6-infected progenitor cells by polymerase chain reaction and in situ hybridization, direct effects of HHV-6 on the hematopoietic progenitor cells are suggested as the cause of the suppression rather than indirect effects via accessory cells of the bone marrow.",
keywords = "Bone marrow transplantation, Burst forming unit erythroid, Colony forming unit granulocyte/macrophage, Human herpesvirus 7, In situ hybridization, Polymerase chain reaction",
author = "Hiroki Isomura and Masao Yamada and Mariko Yoshida and Hiroyuki Tanaka and Tetsuro Kitamura and Megumi Oda and Shiro Nii and Yoshiki Seino",
year = "1997",
month = "8",
doi = "10.1002/(SICI)1096-9071(199708)52:4<406::AID-JMV11>3.0.CO;2-E",
language = "English",
volume = "52",
pages = "406--412",
journal = "Journal of Medical Virology",
issn = "0146-6615",
publisher = "Wiley-Liss Inc.",
number = "4",

}

TY - JOUR

T1 - Suppressive effects of human herpesvirus 6 on in vitro colony formation of hematopoietic progenitor cells

AU - Isomura, Hiroki

AU - Yamada, Masao

AU - Yoshida, Mariko

AU - Tanaka, Hiroyuki

AU - Kitamura, Tetsuro

AU - Oda, Megumi

AU - Nii, Shiro

AU - Seino, Yoshiki

PY - 1997/8

Y1 - 1997/8

N2 - Human herpesvirus 6 (HHV-6) has been reported to be involved in bone marrow failure after bone marrow transplantation (BMT). To elucidate the role of HHV-6 in the marrow failure, we examined the comparative effect of two variants of HHV-6 (HHV-6A and HHV-6B) and human herpesvirus 7 (HHV-7) on in vitro colony formation of hematopoietic progenitor cells in methylcellulose semi-solid media. Progenitor cells prepared from cord blood mononuclear cells (CBMNCs) were infected with one of these viruses at various multiplicity of infection (MOI), and were subjected to methylcellulose colony assay. Formation of both granulocyte/macrophage (CFU-GM) and erythroid (BFU-E) colonies was MOI-dependently suppressed after infection with the Z29 strain of HHV-6B. Although HHV-6A suppressed the formation of BFU-E colonies as efficiently as HHV-6B, the former did not exhibit significant suppressive effect on the formation of CFU-GM colonies at an MOI 1. HHV-7 had no effect on hematopoietic colony formation at all. Based on frequent positivity of viral DNA in single colonies obtained from HHV-6-infected progenitor cells by polymerase chain reaction and in situ hybridization, direct effects of HHV-6 on the hematopoietic progenitor cells are suggested as the cause of the suppression rather than indirect effects via accessory cells of the bone marrow.

AB - Human herpesvirus 6 (HHV-6) has been reported to be involved in bone marrow failure after bone marrow transplantation (BMT). To elucidate the role of HHV-6 in the marrow failure, we examined the comparative effect of two variants of HHV-6 (HHV-6A and HHV-6B) and human herpesvirus 7 (HHV-7) on in vitro colony formation of hematopoietic progenitor cells in methylcellulose semi-solid media. Progenitor cells prepared from cord blood mononuclear cells (CBMNCs) were infected with one of these viruses at various multiplicity of infection (MOI), and were subjected to methylcellulose colony assay. Formation of both granulocyte/macrophage (CFU-GM) and erythroid (BFU-E) colonies was MOI-dependently suppressed after infection with the Z29 strain of HHV-6B. Although HHV-6A suppressed the formation of BFU-E colonies as efficiently as HHV-6B, the former did not exhibit significant suppressive effect on the formation of CFU-GM colonies at an MOI 1. HHV-7 had no effect on hematopoietic colony formation at all. Based on frequent positivity of viral DNA in single colonies obtained from HHV-6-infected progenitor cells by polymerase chain reaction and in situ hybridization, direct effects of HHV-6 on the hematopoietic progenitor cells are suggested as the cause of the suppression rather than indirect effects via accessory cells of the bone marrow.

KW - Bone marrow transplantation

KW - Burst forming unit erythroid

KW - Colony forming unit granulocyte/macrophage

KW - Human herpesvirus 7

KW - In situ hybridization

KW - Polymerase chain reaction

UR - http://www.scopus.com/inward/record.url?scp=0030873349&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030873349&partnerID=8YFLogxK

U2 - 10.1002/(SICI)1096-9071(199708)52:4<406::AID-JMV11>3.0.CO;2-E

DO - 10.1002/(SICI)1096-9071(199708)52:4<406::AID-JMV11>3.0.CO;2-E

M3 - Article

VL - 52

SP - 406

EP - 412

JO - Journal of Medical Virology

JF - Journal of Medical Virology

SN - 0146-6615

IS - 4

ER -