Suppressive effect of asbestos on cytotoxicity of human NK cells.

Y. Nishimura, N. Kumagai, Megumi Maeda, H. Hayashi, K. Fukuoka, T. Nakano, Y. Miura, J. Hiratsuka, T. Otsuki

Research output: Contribution to journalArticle

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Abstract

Asbestos, a naturally occurring fibrous mineral, causes malignant mesothelioma (MM). However, it takes a very long time to develop MM, which suggests that effects other than tumorigenicity of asbestos might contribute to the development of MM, and one of the possible targets is anti-tumor immunity. Therefore, we examined the effect of asbestos exposure on human natural killer (NK) cells using the cell line of YT-A1, Peripheral blood mononuclear cells (PBMCs) cultures and specimens from patients with MM. In particular, we focused on expression of NK cell-activating receptors, including NKG2D, 2B4 and NKp46. Analysis of the YT-CB5 subline of YT-A1, cultured with CB for over 5 months, showed a decrease in cytotoxicity with low expressions of NKG2D and 2B4, although there were no decreases after about one month. YT-CB5 showed decreases in phosphorylation of extracellular signal-regulated kinase (ERK) and degranulation stimulated by antibodies to NKG2D. Peripheral blood (PB-) NK cells from MM patients also showed decreased cytotoxicity compared with healthy volunteers (HV), and was accompanied with low expression of NKp46 unlike YT-CB5. PBMCs cultured with CB resulted in decreased expression of NKp46 on NK cells, although this did not occur when using glass wool, an asbestos substitute. These results indicate that asbestos has the potential to suppress cytotoxicity of NK cells. In particular, it is noteworthy that both NK cells from MM patients and those from a culture of PBMCs derived from HVs with asbestos showed the same characteristic of decreased cytotoxicity with low expression of NKp46.

Original languageEnglish
JournalInternational Journal of Immunopathology and Pharmacology
Volume24
Issue number1 Suppl
Publication statusPublished - Jan 2011
Externally publishedYes

Fingerprint

Asbestos
Natural Killer Cells
Blood Cells
Natural Killer Cell Receptors
Wool
Extracellular Signal-Regulated MAP Kinases
Glass
Minerals
Malignant Mesothelioma
Immunity
Healthy Volunteers
Cell Culture Techniques
Phosphorylation
Cell Line
Antibodies
Neoplasms

ASJC Scopus subject areas

  • Pharmacology
  • Immunology
  • Immunology and Allergy

Cite this

Nishimura, Y., Kumagai, N., Maeda, M., Hayashi, H., Fukuoka, K., Nakano, T., ... Otsuki, T. (2011). Suppressive effect of asbestos on cytotoxicity of human NK cells. International Journal of Immunopathology and Pharmacology, 24(1 Suppl).

Suppressive effect of asbestos on cytotoxicity of human NK cells. / Nishimura, Y.; Kumagai, N.; Maeda, Megumi; Hayashi, H.; Fukuoka, K.; Nakano, T.; Miura, Y.; Hiratsuka, J.; Otsuki, T.

In: International Journal of Immunopathology and Pharmacology, Vol. 24, No. 1 Suppl, 01.2011.

Research output: Contribution to journalArticle

Nishimura, Y, Kumagai, N, Maeda, M, Hayashi, H, Fukuoka, K, Nakano, T, Miura, Y, Hiratsuka, J & Otsuki, T 2011, 'Suppressive effect of asbestos on cytotoxicity of human NK cells.', International Journal of Immunopathology and Pharmacology, vol. 24, no. 1 Suppl.
Nishimura, Y. ; Kumagai, N. ; Maeda, Megumi ; Hayashi, H. ; Fukuoka, K. ; Nakano, T. ; Miura, Y. ; Hiratsuka, J. ; Otsuki, T. / Suppressive effect of asbestos on cytotoxicity of human NK cells. In: International Journal of Immunopathology and Pharmacology. 2011 ; Vol. 24, No. 1 Suppl.
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