Suppression of Th17 response by Streptococcus thermophilus ST28 through induction of IFN-γ

Tasuku Ogita, Yusuke Tanii, Hidetoshi Morita, Takuya Suzuki, Soichi Tanabe

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The proinflammatory cytokine interleukin (IL)-17 plays important roles in various inflammatory diseases, and IL-17-producing T helper 17 cells (Th17) have received much attention. For therapy of Th17-mediated diseases, some reports have indicated the clinical efficacy of lactic acid bacteria, including Streptococcus thermophilus. In this study, we examined the mechanism for the suppressive effects of S. thermophilus ST28 on the Th17 response in murine splenocytes stimulated with transforming growth factor (TGF)-β plus IL-6.Stimulation with TGF-β plus IL-6 increased mRNA expression of IL-17 and its production in the splenocytes, but ST28 markedly suppressed both. Meanwhile, ST28 increased the mRNA expression of interferon (IFN)-γ as well as its production. Anti-IFN-γ completely cancelled the suppressive effect of ST28 on IL-17 production. From these data, it was concluded that IFN-γinduced by ST28 had an important role on the effect. A genomic DNA (10 μg/ml) from ST28 effectively suppressed IL-17 production, probably via the Toll-like receptor 9. Therefore, modulation of Th1/Th17 balance would be one of the mechanisms under which S. thermophilus ST28 exerts an anti-inflammatory effect.

Original languageEnglish
Pages (from-to)817-822
Number of pages6
JournalInternational Journal of Molecular Medicine
Volume28
Issue number5
DOIs
Publication statusPublished - Nov 2011
Externally publishedYes

Fingerprint

Streptococcus thermophilus
Th17 Cells
Interleukin-17
Interferons
Transforming Growth Factors
Interleukin-6
Toll-Like Receptor 9
Messenger RNA
Lactic Acid
Anti-Inflammatory Agents
Cytokines
Bacteria
DNA

Keywords

  • Interleukin-17
  • Streptococcus thermophilus
  • T helper 17 cells

ASJC Scopus subject areas

  • Genetics

Cite this

Suppression of Th17 response by Streptococcus thermophilus ST28 through induction of IFN-γ. / Ogita, Tasuku; Tanii, Yusuke; Morita, Hidetoshi; Suzuki, Takuya; Tanabe, Soichi.

In: International Journal of Molecular Medicine, Vol. 28, No. 5, 11.2011, p. 817-822.

Research output: Contribution to journalArticle

Ogita, Tasuku ; Tanii, Yusuke ; Morita, Hidetoshi ; Suzuki, Takuya ; Tanabe, Soichi. / Suppression of Th17 response by Streptococcus thermophilus ST28 through induction of IFN-γ. In: International Journal of Molecular Medicine. 2011 ; Vol. 28, No. 5. pp. 817-822.
@article{c6f42673297346cd91494dbe60a4f6e5,
title = "Suppression of Th17 response by Streptococcus thermophilus ST28 through induction of IFN-γ",
abstract = "The proinflammatory cytokine interleukin (IL)-17 plays important roles in various inflammatory diseases, and IL-17-producing T helper 17 cells (Th17) have received much attention. For therapy of Th17-mediated diseases, some reports have indicated the clinical efficacy of lactic acid bacteria, including Streptococcus thermophilus. In this study, we examined the mechanism for the suppressive effects of S. thermophilus ST28 on the Th17 response in murine splenocytes stimulated with transforming growth factor (TGF)-β plus IL-6.Stimulation with TGF-β plus IL-6 increased mRNA expression of IL-17 and its production in the splenocytes, but ST28 markedly suppressed both. Meanwhile, ST28 increased the mRNA expression of interferon (IFN)-γ as well as its production. Anti-IFN-γ completely cancelled the suppressive effect of ST28 on IL-17 production. From these data, it was concluded that IFN-γinduced by ST28 had an important role on the effect. A genomic DNA (10 μg/ml) from ST28 effectively suppressed IL-17 production, probably via the Toll-like receptor 9. Therefore, modulation of Th1/Th17 balance would be one of the mechanisms under which S. thermophilus ST28 exerts an anti-inflammatory effect.",
keywords = "Interleukin-17, Streptococcus thermophilus, T helper 17 cells",
author = "Tasuku Ogita and Yusuke Tanii and Hidetoshi Morita and Takuya Suzuki and Soichi Tanabe",
year = "2011",
month = "11",
doi = "10.3892/ijmm.2011.755",
language = "English",
volume = "28",
pages = "817--822",
journal = "International Journal of Molecular Medicine",
issn = "1107-3756",
publisher = "Spandidos Publications",
number = "5",

}

TY - JOUR

T1 - Suppression of Th17 response by Streptococcus thermophilus ST28 through induction of IFN-γ

AU - Ogita, Tasuku

AU - Tanii, Yusuke

AU - Morita, Hidetoshi

AU - Suzuki, Takuya

AU - Tanabe, Soichi

PY - 2011/11

Y1 - 2011/11

N2 - The proinflammatory cytokine interleukin (IL)-17 plays important roles in various inflammatory diseases, and IL-17-producing T helper 17 cells (Th17) have received much attention. For therapy of Th17-mediated diseases, some reports have indicated the clinical efficacy of lactic acid bacteria, including Streptococcus thermophilus. In this study, we examined the mechanism for the suppressive effects of S. thermophilus ST28 on the Th17 response in murine splenocytes stimulated with transforming growth factor (TGF)-β plus IL-6.Stimulation with TGF-β plus IL-6 increased mRNA expression of IL-17 and its production in the splenocytes, but ST28 markedly suppressed both. Meanwhile, ST28 increased the mRNA expression of interferon (IFN)-γ as well as its production. Anti-IFN-γ completely cancelled the suppressive effect of ST28 on IL-17 production. From these data, it was concluded that IFN-γinduced by ST28 had an important role on the effect. A genomic DNA (10 μg/ml) from ST28 effectively suppressed IL-17 production, probably via the Toll-like receptor 9. Therefore, modulation of Th1/Th17 balance would be one of the mechanisms under which S. thermophilus ST28 exerts an anti-inflammatory effect.

AB - The proinflammatory cytokine interleukin (IL)-17 plays important roles in various inflammatory diseases, and IL-17-producing T helper 17 cells (Th17) have received much attention. For therapy of Th17-mediated diseases, some reports have indicated the clinical efficacy of lactic acid bacteria, including Streptococcus thermophilus. In this study, we examined the mechanism for the suppressive effects of S. thermophilus ST28 on the Th17 response in murine splenocytes stimulated with transforming growth factor (TGF)-β plus IL-6.Stimulation with TGF-β plus IL-6 increased mRNA expression of IL-17 and its production in the splenocytes, but ST28 markedly suppressed both. Meanwhile, ST28 increased the mRNA expression of interferon (IFN)-γ as well as its production. Anti-IFN-γ completely cancelled the suppressive effect of ST28 on IL-17 production. From these data, it was concluded that IFN-γinduced by ST28 had an important role on the effect. A genomic DNA (10 μg/ml) from ST28 effectively suppressed IL-17 production, probably via the Toll-like receptor 9. Therefore, modulation of Th1/Th17 balance would be one of the mechanisms under which S. thermophilus ST28 exerts an anti-inflammatory effect.

KW - Interleukin-17

KW - Streptococcus thermophilus

KW - T helper 17 cells

UR - http://www.scopus.com/inward/record.url?scp=80053117777&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80053117777&partnerID=8YFLogxK

U2 - 10.3892/ijmm.2011.755

DO - 10.3892/ijmm.2011.755

M3 - Article

C2 - 21887477

AN - SCOPUS:80053117777

VL - 28

SP - 817

EP - 822

JO - International Journal of Molecular Medicine

JF - International Journal of Molecular Medicine

SN - 1107-3756

IS - 5

ER -