Suppression of pisatin production and ATPase activity in pea plasma membranes by orthovanadate, verapamil and a suppressor from mycosphaerella pinodes

Hirofumi Yoshioka, Tomonori Shiraishi, Tetsuji Yamada, Yuki Ichinose, Hachiro Oku

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

A pea pathogen, Mycosphaerella pinodes, secretes both an elicitor and a suppressor for the accumulation of pisatin, a major phytoalexin of pea, into the spore germination fluid. The effects of the elicitor and the suppressor on the ATPase activity in pea plasma membranes was examined. The ATPase was sensitive to orthovanadate and dicyclohexylcarbodiimide but insensitive to nitrate and azide; it was unaffected by the elicitor but was markedly inhibited by the suppressor (50μg.ml-1, bovine serum albumin equivalents) or verapamil (1OOμM). The accumulation of pisatin induced by the elicitor was delayed for 3 to 6 h in the presence of orthovanadate or verapamil to an extent similar to that in the presence of the suppressor. The relationship between the inhibition of plasma membrane ATPase activity and the suppression of the active defense reaction that involves the production of pisatin in the pea plant is discussed.

Original languageEnglish
Pages (from-to)1139-1146
Number of pages8
JournalPlant and Cell Physiology
Volume31
Issue number8
Publication statusPublished - 1990

Fingerprint

pisatin
Mycosphaerella pinodes
Verapamil
verapamil
Plasma Membrane
Vanadates
ATP Synthase
Peas
Cell membranes
adenosinetriphosphatase
Adenosine Triphosphatases
peas
plasma membrane
Cell Membrane
membrane
plasma
spore
serum
germination
pathogen

Keywords

  • ATPase
  • Mycosphaerella pinodes
  • Pisatin
  • Pisum sativum L
  • Suppressor
  • Verapamil

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Ecology
  • Cell Biology
  • Physiology
  • Plant Science

Cite this

Suppression of pisatin production and ATPase activity in pea plasma membranes by orthovanadate, verapamil and a suppressor from mycosphaerella pinodes. / Yoshioka, Hirofumi; Shiraishi, Tomonori; Yamada, Tetsuji; Ichinose, Yuki; Oku, Hachiro.

In: Plant and Cell Physiology, Vol. 31, No. 8, 1990, p. 1139-1146.

Research output: Contribution to journalArticle

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