Suppression of mRNAs for lipoxygenase (LOX), allene oxide synthase (AOS), allene oxide cyclase (AOC) and 12-oxo-phytodienoic acid reductase (OPR) in pea reduces sensitivity to the phytotoxin coronatine and disease development by Mycosphaerella pinodes

Kazuhiro Toyoda, Yuriko Kawanishi, Yuriko Kawamoto, Chiaki Kurihara, Noriko Yamagishi, Akihiro Tamura, Nobuyuki Yoshikawa, Yoshishige Inagaki, Yuki Ichinose, Tomonori Shiraishi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Using a recently developed model pathosystem involving Medicago truncatula and Mycosphaerella pinodes, causal agent of Mycosphaerella blight on pea to understand host molecular response to a fungal suppressor, we applied the suppressor to leaves of M. truncatula and identified 151 nonredundant cDNA fragments as newly expressed genes. These included genes encoding lipoxygenase (LOX) and enoyl-CoA hydratase, which are presumably involved in jasmonic acid (JA) synthesis. Potential genes encoding plastidic enzymes, including allene oxide synthase (AOS) and allene oxide cyclase (AOC), and other peroxisomal enzymes involved in β-oxidation were predicted from the Medicago Gene Index EST database and tested for altered expression by semiquantitative RT-PCR. The coordinated expression of genes encoding both plastidic and peroxisomal enzymes showed that the suppressor likely conditions certain cellular process(es) through the JA synthesis in M. truncatula. To explore the role of JA or JA-regulated cellular process(es) in conditioning susceptibility, we used an Apple latent spherical virus (ALSV)-based virus-induced gene silencing (VIGS) technology to silence pea genes including LOX, AOS, AOC and 12-oxo-phytodienoic acid reductase (OPR). In LOX-, AOS-, AOC- or OPR-silenced pea plants, disease development induced by M. pinodes was remarkably reduced. Similarly, silencing of mRNA for LOX, AOS, AOC or OPR reduced the sensitivity to a phytotoxin, coronatine, which is believed to act through a JA-dependent process. On the basis of these results, it is conceivable that M. pinodes has evolved a strategy to condition susceptibility by manipulating the physiology of host cells, in particular JA-regulated cellular process(es), to promote disease development in pea.

Original languageEnglish
Pages (from-to)321-334
Number of pages14
JournalJournal of General Plant Pathology
Volume79
Issue number5
DOIs
Publication statusPublished - Sep 2013

Fingerprint

Mycosphaerella pinodes
phytotoxins
jasmonic acid
lipoxygenase
peas
Medicago truncatula
acids
genes
Apple latent spherical virus
enzymes
Mycosphaerella
Medicago
synthesis
gene silencing
plant diseases and disorders
blight
hydroperoxide dehydratase
allene-oxide cyclase
physiology
reverse transcriptase polymerase chain reaction

Keywords

  • Apple latent spherical virus (ALSV) vector
  • Disease susceptibility
  • Mycosphaerella pinodes
  • Pea (Pisum sativum L.)
  • Suppression subtractive hybridization (SSH)
  • Suppressor

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science

Cite this

Suppression of mRNAs for lipoxygenase (LOX), allene oxide synthase (AOS), allene oxide cyclase (AOC) and 12-oxo-phytodienoic acid reductase (OPR) in pea reduces sensitivity to the phytotoxin coronatine and disease development by Mycosphaerella pinodes. / Toyoda, Kazuhiro; Kawanishi, Yuriko; Kawamoto, Yuriko; Kurihara, Chiaki; Yamagishi, Noriko; Tamura, Akihiro; Yoshikawa, Nobuyuki; Inagaki, Yoshishige; Ichinose, Yuki; Shiraishi, Tomonori.

In: Journal of General Plant Pathology, Vol. 79, No. 5, 09.2013, p. 321-334.

Research output: Contribution to journalArticle

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