Suppression of liver microsomal drug-metabolizing enzyme activities in adult female rats pretreated with cannabidiol

S. Narimatsu, K. Watanabe, T. Matsunaga, I. Yamamoto, S. Imaoka, Y. Funae, H. Yoshimura

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The suppression by cannabidiol (CBD) of the liver microsomal drug-metabolizing enzyme activities in female rats was demonstrated and its mechanism was examined. Pretreatment of rats with CBD (10 mg/kg, i.p.) caused temporary decreases in contents of cytochrome P450 (P450) and b5 and NADPH-cytochrome c (P450) reductase activity compared with values from the vehicle control group. p-Nitroanisole O-demethylase, aniline hydroxylase, d-benzphetamine N-demethylase and Δ9-tetrahydrocannabinol 11-hydroxylase were also decreased by the CBD pretreatment. The latter two activities took a longer time to return to control levels than the former two. However, the CBD pretreatment, which reduced the protein level of P450 UT-2 (CYP2C11) in adult male rats, did not decrease the protein level of P450 E-1 (CYP2C6) or E-2 (CYP2C12) in liver microsomes from female rats. These results suggest that the mechanisms by which CBD suppresses liver microsomal drug-metabolizing enzyme activities are different in male and female rats.

Original languageEnglish
Pages (from-to)428-430
Number of pages3
JournalBiological and Pharmaceutical Bulletin
Volume16
Issue number4
Publication statusPublished - 1993
Externally publishedYes

Fingerprint

Cannabidiol
Liver
Enzymes
NADPH-Ferrihemoprotein Reductase
Pharmaceutical Preparations
Aniline Hydroxylase
Dronabinol
Liver Microsomes
Mixed Function Oxygenases
Cytochrome P-450 Enzyme System
Proteins
Control Groups

Keywords

  • Δ-tetrahydrocannabinol
  • cannabidiol
  • cytochrome P450
  • d-benzphetamine
  • rat liver microsome
  • suppressive effect

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Narimatsu, S., Watanabe, K., Matsunaga, T., Yamamoto, I., Imaoka, S., Funae, Y., & Yoshimura, H. (1993). Suppression of liver microsomal drug-metabolizing enzyme activities in adult female rats pretreated with cannabidiol. Biological and Pharmaceutical Bulletin, 16(4), 428-430.

Suppression of liver microsomal drug-metabolizing enzyme activities in adult female rats pretreated with cannabidiol. / Narimatsu, S.; Watanabe, K.; Matsunaga, T.; Yamamoto, I.; Imaoka, S.; Funae, Y.; Yoshimura, H.

In: Biological and Pharmaceutical Bulletin, Vol. 16, No. 4, 1993, p. 428-430.

Research output: Contribution to journalArticle

Narimatsu, S, Watanabe, K, Matsunaga, T, Yamamoto, I, Imaoka, S, Funae, Y & Yoshimura, H 1993, 'Suppression of liver microsomal drug-metabolizing enzyme activities in adult female rats pretreated with cannabidiol', Biological and Pharmaceutical Bulletin, vol. 16, no. 4, pp. 428-430.
Narimatsu, S. ; Watanabe, K. ; Matsunaga, T. ; Yamamoto, I. ; Imaoka, S. ; Funae, Y. ; Yoshimura, H. / Suppression of liver microsomal drug-metabolizing enzyme activities in adult female rats pretreated with cannabidiol. In: Biological and Pharmaceutical Bulletin. 1993 ; Vol. 16, No. 4. pp. 428-430.
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