Abstract
The suppression by cannabidiol (CBD) of the liver microsomal drug-metabolizing enzyme activities in female rats was demonstrated and its mechanism was examined. Pretreatment of rats with CBD (10 mg/kg, i.p.) caused temporary decreases in contents of cytochrome P450 (P450) and b5 and NADPH-cytochrome c (P450) reductase activity compared with values from the vehicle control group. p-Nitroanisole O-demethylase, aniline hydroxylase, d-benzphetamine N-demethylase and Δ9-tetrahydrocannabinol 11-hydroxylase were also decreased by the CBD pretreatment. The latter two activities took a longer time to return to control levels than the former two. However, the CBD pretreatment, which reduced the protein level of P450 UT-2 (CYP2C11) in adult male rats, did not decrease the protein level of P450 E-1 (CYP2C6) or E-2 (CYP2C12) in liver microsomes from female rats. These results suggest that the mechanisms by which CBD suppresses liver microsomal drug-metabolizing enzyme activities are different in male and female rats.
| Original language | English |
|---|---|
| Pages (from-to) | 428-430 |
| Number of pages | 3 |
| Journal | Biological and Pharmaceutical Bulletin |
| Volume | 16 |
| Issue number | 4 |
| Publication status | Published - 1993 |
| Externally published | Yes |
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Keywords
- Δ-tetrahydrocannabinol
- cannabidiol
- cytochrome P450
- d-benzphetamine
- rat liver microsome
- suppressive effect
ASJC Scopus subject areas
- Molecular Medicine
- Pharmacology, Toxicology and Pharmaceutics(all)
Cite this
Suppression of liver microsomal drug-metabolizing enzyme activities in adult female rats pretreated with cannabidiol. / Narimatsu, S.; Watanabe, K.; Matsunaga, T.; Yamamoto, I.; Imaoka, S.; Funae, Y.; Yoshimura, H.
In: Biological and Pharmaceutical Bulletin, Vol. 16, No. 4, 1993, p. 428-430.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Suppression of liver microsomal drug-metabolizing enzyme activities in adult female rats pretreated with cannabidiol
AU - Narimatsu, S.
AU - Watanabe, K.
AU - Matsunaga, T.
AU - Yamamoto, I.
AU - Imaoka, S.
AU - Funae, Y.
AU - Yoshimura, H.
PY - 1993
Y1 - 1993
N2 - The suppression by cannabidiol (CBD) of the liver microsomal drug-metabolizing enzyme activities in female rats was demonstrated and its mechanism was examined. Pretreatment of rats with CBD (10 mg/kg, i.p.) caused temporary decreases in contents of cytochrome P450 (P450) and b5 and NADPH-cytochrome c (P450) reductase activity compared with values from the vehicle control group. p-Nitroanisole O-demethylase, aniline hydroxylase, d-benzphetamine N-demethylase and Δ9-tetrahydrocannabinol 11-hydroxylase were also decreased by the CBD pretreatment. The latter two activities took a longer time to return to control levels than the former two. However, the CBD pretreatment, which reduced the protein level of P450 UT-2 (CYP2C11) in adult male rats, did not decrease the protein level of P450 E-1 (CYP2C6) or E-2 (CYP2C12) in liver microsomes from female rats. These results suggest that the mechanisms by which CBD suppresses liver microsomal drug-metabolizing enzyme activities are different in male and female rats.
AB - The suppression by cannabidiol (CBD) of the liver microsomal drug-metabolizing enzyme activities in female rats was demonstrated and its mechanism was examined. Pretreatment of rats with CBD (10 mg/kg, i.p.) caused temporary decreases in contents of cytochrome P450 (P450) and b5 and NADPH-cytochrome c (P450) reductase activity compared with values from the vehicle control group. p-Nitroanisole O-demethylase, aniline hydroxylase, d-benzphetamine N-demethylase and Δ9-tetrahydrocannabinol 11-hydroxylase were also decreased by the CBD pretreatment. The latter two activities took a longer time to return to control levels than the former two. However, the CBD pretreatment, which reduced the protein level of P450 UT-2 (CYP2C11) in adult male rats, did not decrease the protein level of P450 E-1 (CYP2C6) or E-2 (CYP2C12) in liver microsomes from female rats. These results suggest that the mechanisms by which CBD suppresses liver microsomal drug-metabolizing enzyme activities are different in male and female rats.
KW - Δ-tetrahydrocannabinol
KW - cannabidiol
KW - cytochrome P450
KW - d-benzphetamine
KW - rat liver microsome
KW - suppressive effect
UR - http://www.scopus.com/inward/record.url?scp=0027163057&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027163057&partnerID=8YFLogxK
M3 - Article
C2 - 8395282
AN - SCOPUS:0027163057
VL - 16
SP - 428
EP - 430
JO - Biological and Pharmaceutical Bulletin
JF - Biological and Pharmaceutical Bulletin
SN - 0918-6158
IS - 4
ER -