Suppression of liver microsomal drug-metabolizing enzyme activities in adult female rats pretreated with cannabidiol

S. Narimatsu, K. Watanabe, T. Matsunaga, I. Yamamoto, S. Imaoka, Y. Funae, H. Yoshimura

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Abstract

The suppression by cannabidiol (CBD) of the liver microsomal drug-metabolizing enzyme activities in female rats was demonstrated and its mechanism was examined. Pretreatment of rats with CBD (10 mg/kg, i.p.) caused temporary decreases in contents of cytochrome P450 (P450) and b5 and NADPH-cytochrome c (P450) reductase activity compared with values from the vehicle control group. p-Nitroanisole O-demethylase, aniline hydroxylase, d-benzphetamine N-demethylase and Δ9-tetrahydrocannabinol 11-hydroxylase were also decreased by the CBD pretreatment. The latter two activities took a longer time to return to control levels than the former two. However, the CBD pretreatment, which reduced the protein level of P450 UT-2 (CYP2C11) in adult male rats, did not decrease the protein level of P450 E-1 (CYP2C6) or E-2 (CYP2C12) in liver microsomes from female rats. These results suggest that the mechanisms by which CBD suppresses liver microsomal drug-metabolizing enzyme activities are different in male and female rats.

Original languageEnglish
Pages (from-to)428-430
Number of pages3
JournalBiological and Pharmaceutical Bulletin
Volume16
Issue number4
Publication statusPublished - 1993
Externally publishedYes

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Keywords

  • Δ-tetrahydrocannabinol
  • cannabidiol
  • cytochrome P450
  • d-benzphetamine
  • rat liver microsome
  • suppressive effect

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Narimatsu, S., Watanabe, K., Matsunaga, T., Yamamoto, I., Imaoka, S., Funae, Y., & Yoshimura, H. (1993). Suppression of liver microsomal drug-metabolizing enzyme activities in adult female rats pretreated with cannabidiol. Biological and Pharmaceutical Bulletin, 16(4), 428-430.