Suppression of genotoxicity of carcinogens by (-)-epigallocatechin gallate

Hikoya Hayatsu, Naomi Inada, Toshifumi Kakutani, Sakae Arimoto, Tomoe Negishi, Kazuko Mori, Takuo Okuda, Isao Sakata

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Epidemiological evidence shows that green tea may be a factor in lowering cancer risk. We have investigated the possibility that (-)-epigallocatechin gallate (EGCG), a major polyphenol in green tea, might be an antimutagenic substance. In the Ames Salmonella test, EGCG suppressed the direct-acting mutagenicity of 3-hydroxyamino-1-methyl-5H-pyrido-[4,3-b]indole (Trp-P-2(NHOH)) and 2-hydroxyamino-6-methyldipyrido[1,2-a:3′,2′-d]imidazole (Glu-P-1(NHOH)), the activated forms of food-derived carcinogens 3-amino-1-methyl-5H-pyrido[4,3-b]indole and 2-amino-6-methyldipyrido[1,2-a:3′,2′-d]imidazole. EGCG was also effective in reducing the mutagenicity of Trp-P-2(NHOH) in mouse FM3A cells in culture. Furthermore, EGCG demonstrated a suppressive effect in the in vivo Drosophila mutation assays, i.e., the wing spot test, and the DNA repair test, on several carcinogens. EGCG was also effective in inhibiting DNA single-strand breaks in vitro caused by Glu-P-1(NHOH). We conclude that the mechanism of inhibition may not have resulted from direct interaction between EGCG and the mutagens, but rather from indirect interception of mutagen action by EGCG.

Original languageEnglish
Pages (from-to)370-376
Number of pages7
JournalPreventive Medicine
Volume21
Issue number3
DOIs
Publication statusPublished - May 1992

ASJC Scopus subject areas

  • Epidemiology
  • Public Health, Environmental and Occupational Health

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