Suppression of electroosmotic flow and its application to determination of electrophoretic mobilities in a poly(vinylpyrrolidone)-coated capillary

Takashi Kaneta, Takeshi Ueda, Kazuki Hata, Totaro Imasaka

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

A hydrophilic polymer, poly(vinylpyrrolidone) (PVP), was employed for suppressing the electroosmotic flow (EOF). A capillary was filled with aqueous PVP solution for coating the capillary wall with PVP; the PVP solution was then replaced by a migration buffer solution containing no PVP. Three types of PVP with different molecular weights were examined. The EOF was suppressed more effectively as the molecular weight of PVP increased. The EOF in the coated capillary was ∼10-fold smaller than that of a bare capillary and was constant in the pH range of 6-8. The suppressed EOF was stable even when no PVP was added to the migration buffer. However, the EOF increased significantly when sodium dodecyl sulfate was added into the migration buffer. The method was applied for determining the electrophoretic mobilities of inorganic anions that have negative electrophoretic mobilities larger than the electroosmotic mobility of the bare capillary. A novel method for determining the electrophoretic mobilities was proposed based on the linear relationship between electric current and electrophoretic mobility. The electrophoretic mobility was proportional to the electric current. Therefore, the intercept of the regression equation represents the electrophoretic mobility at room temperature. The electrophoretic mobilities were in good agreement with the absolute electrophoretic mobilities.

Original languageEnglish
Pages (from-to)52-55
Number of pages4
JournalJournal of Chromatography A
Volume1106
Issue number1-2
DOIs
Publication statusPublished - Feb 17 2006
Externally publishedYes

Fingerprint

Electroosmosis
Electrophoretic mobility
Buffers
Molecular Weight
Electric currents
Sodium Dodecyl Sulfate
Molecular weight
Anions
Polymers
Temperature
Coatings

Keywords

  • Capillary electrophoresis
  • Dynamic coating
  • Electroosmotic flow
  • Electrophoretic mobility
  • Inorganic anion
  • Poly(vinylpyrrolidone)

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Suppression of electroosmotic flow and its application to determination of electrophoretic mobilities in a poly(vinylpyrrolidone)-coated capillary. / Kaneta, Takashi; Ueda, Takeshi; Hata, Kazuki; Imasaka, Totaro.

In: Journal of Chromatography A, Vol. 1106, No. 1-2, 17.02.2006, p. 52-55.

Research output: Contribution to journalArticle

@article{dc21cb98c3a2412496addeb7f29f99bb,
title = "Suppression of electroosmotic flow and its application to determination of electrophoretic mobilities in a poly(vinylpyrrolidone)-coated capillary",
abstract = "A hydrophilic polymer, poly(vinylpyrrolidone) (PVP), was employed for suppressing the electroosmotic flow (EOF). A capillary was filled with aqueous PVP solution for coating the capillary wall with PVP; the PVP solution was then replaced by a migration buffer solution containing no PVP. Three types of PVP with different molecular weights were examined. The EOF was suppressed more effectively as the molecular weight of PVP increased. The EOF in the coated capillary was ∼10-fold smaller than that of a bare capillary and was constant in the pH range of 6-8. The suppressed EOF was stable even when no PVP was added to the migration buffer. However, the EOF increased significantly when sodium dodecyl sulfate was added into the migration buffer. The method was applied for determining the electrophoretic mobilities of inorganic anions that have negative electrophoretic mobilities larger than the electroosmotic mobility of the bare capillary. A novel method for determining the electrophoretic mobilities was proposed based on the linear relationship between electric current and electrophoretic mobility. The electrophoretic mobility was proportional to the electric current. Therefore, the intercept of the regression equation represents the electrophoretic mobility at room temperature. The electrophoretic mobilities were in good agreement with the absolute electrophoretic mobilities.",
keywords = "Capillary electrophoresis, Dynamic coating, Electroosmotic flow, Electrophoretic mobility, Inorganic anion, Poly(vinylpyrrolidone)",
author = "Takashi Kaneta and Takeshi Ueda and Kazuki Hata and Totaro Imasaka",
year = "2006",
month = "2",
day = "17",
doi = "10.1016/j.chroma.2005.08.062",
language = "English",
volume = "1106",
pages = "52--55",
journal = "Journal of Chromatography",
issn = "0021-9673",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Suppression of electroosmotic flow and its application to determination of electrophoretic mobilities in a poly(vinylpyrrolidone)-coated capillary

AU - Kaneta, Takashi

AU - Ueda, Takeshi

AU - Hata, Kazuki

AU - Imasaka, Totaro

PY - 2006/2/17

Y1 - 2006/2/17

N2 - A hydrophilic polymer, poly(vinylpyrrolidone) (PVP), was employed for suppressing the electroosmotic flow (EOF). A capillary was filled with aqueous PVP solution for coating the capillary wall with PVP; the PVP solution was then replaced by a migration buffer solution containing no PVP. Three types of PVP with different molecular weights were examined. The EOF was suppressed more effectively as the molecular weight of PVP increased. The EOF in the coated capillary was ∼10-fold smaller than that of a bare capillary and was constant in the pH range of 6-8. The suppressed EOF was stable even when no PVP was added to the migration buffer. However, the EOF increased significantly when sodium dodecyl sulfate was added into the migration buffer. The method was applied for determining the electrophoretic mobilities of inorganic anions that have negative electrophoretic mobilities larger than the electroosmotic mobility of the bare capillary. A novel method for determining the electrophoretic mobilities was proposed based on the linear relationship between electric current and electrophoretic mobility. The electrophoretic mobility was proportional to the electric current. Therefore, the intercept of the regression equation represents the electrophoretic mobility at room temperature. The electrophoretic mobilities were in good agreement with the absolute electrophoretic mobilities.

AB - A hydrophilic polymer, poly(vinylpyrrolidone) (PVP), was employed for suppressing the electroosmotic flow (EOF). A capillary was filled with aqueous PVP solution for coating the capillary wall with PVP; the PVP solution was then replaced by a migration buffer solution containing no PVP. Three types of PVP with different molecular weights were examined. The EOF was suppressed more effectively as the molecular weight of PVP increased. The EOF in the coated capillary was ∼10-fold smaller than that of a bare capillary and was constant in the pH range of 6-8. The suppressed EOF was stable even when no PVP was added to the migration buffer. However, the EOF increased significantly when sodium dodecyl sulfate was added into the migration buffer. The method was applied for determining the electrophoretic mobilities of inorganic anions that have negative electrophoretic mobilities larger than the electroosmotic mobility of the bare capillary. A novel method for determining the electrophoretic mobilities was proposed based on the linear relationship between electric current and electrophoretic mobility. The electrophoretic mobility was proportional to the electric current. Therefore, the intercept of the regression equation represents the electrophoretic mobility at room temperature. The electrophoretic mobilities were in good agreement with the absolute electrophoretic mobilities.

KW - Capillary electrophoresis

KW - Dynamic coating

KW - Electroosmotic flow

KW - Electrophoretic mobility

KW - Inorganic anion

KW - Poly(vinylpyrrolidone)

UR - http://www.scopus.com/inward/record.url?scp=31344457172&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=31344457172&partnerID=8YFLogxK

U2 - 10.1016/j.chroma.2005.08.062

DO - 10.1016/j.chroma.2005.08.062

M3 - Article

C2 - 16443452

AN - SCOPUS:31344457172

VL - 1106

SP - 52

EP - 55

JO - Journal of Chromatography

JF - Journal of Chromatography

SN - 0021-9673

IS - 1-2

ER -