Suppression of CXCR4 expression in mast cells upon IgE-mediated antigen stimulation

Junji Matsuura; Mariko Sakanaka; Norio Sato; Atsushi Ichikawa; Satoshi Tanaka

doi:10.1007/s00011-009-0078-7

Suppression of CXCR4 expression in mast cells upon IgE-mediated antigen stimulation

Junji Matsuura, Mariko Sakanaka, Norio Sato, Atsushi Ichikawa, Satoshi Tanaka

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Objective: Recent studies have demonstrated that a variety of chemokine receptors are expressed in mast cells. We investigated the changes in mRNA expression of CXCRs in murine IL-3-dependent bone marrow-derived mast cells (BMMCs) to clarify how the CXCR expression is regulated in mast cells. Methods: Expression of CXCR mRNA was measured by RNase protection assay. Functional expression of CXCRs was confirmed by monitoring intracellular Ca²⁺ mobilization. Results: CXCR4 mRNA expression was transiently induced in BMMCs in serum-dependent fashion and was completely suppressed upon IgE-mediated antigen stimulation. In contrast, CXCR5 mRNA expression was induced upon IgE-mediated antigen stimulation. Changes in the intracellular Ca²⁺ mobilization induced by CXCL12 strongly indicated the functional expression of CXCR4. The decrease in CXCR4 and the increase in CXCR5 mRNA expression was also observed in BMMCs stimulated with thapsigargin, a phorbol ester, and stem cell factor. Conclusion: The mRNA expression of CXCR4 is differentially regulated in BMMCs upon various stimuli including IgE-mediated antigen stimulation.

Original language	English
Pages (from-to)	123-127
Number of pages	5
Journal	Inflammation Research
Volume	59
Issue number	2
DOIs	https://doi.org/10.1007/s00011-009-0078-7
Publication status	Published - Feb 2010
Externally published	Yes

Keywords

CXCR4
CXCR5
Chemokine
Mast cell
SCF

ASJC Scopus subject areas

Immunology
Pharmacology

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title = "Suppression of CXCR4 expression in mast cells upon IgE-mediated antigen stimulation",

abstract = "Objective: Recent studies have demonstrated that a variety of chemokine receptors are expressed in mast cells. We investigated the changes in mRNA expression of CXCRs in murine IL-3-dependent bone marrow-derived mast cells (BMMCs) to clarify how the CXCR expression is regulated in mast cells. Methods: Expression of CXCR mRNA was measured by RNase protection assay. Functional expression of CXCRs was confirmed by monitoring intracellular Ca2+ mobilization. Results: CXCR4 mRNA expression was transiently induced in BMMCs in serum-dependent fashion and was completely suppressed upon IgE-mediated antigen stimulation. In contrast, CXCR5 mRNA expression was induced upon IgE-mediated antigen stimulation. Changes in the intracellular Ca2+ mobilization induced by CXCL12 strongly indicated the functional expression of CXCR4. The decrease in CXCR4 and the increase in CXCR5 mRNA expression was also observed in BMMCs stimulated with thapsigargin, a phorbol ester, and stem cell factor. Conclusion: The mRNA expression of CXCR4 is differentially regulated in BMMCs upon various stimuli including IgE-mediated antigen stimulation.",

keywords = "CXCR4, CXCR5, Chemokine, Mast cell, SCF",

author = "Junji Matsuura and Mariko Sakanaka and Norio Sato and Atsushi Ichikawa and Satoshi Tanaka",

note = "Funding Information: Acknowledgments We thank Dr. A. Popiel for her help in preparation of the manuscript. This study was supported in part by KAKENHI (19590091) Grants-in-Aid for Scientific Research (C) from Japan Society for the Promotion of Science(JSPS).",

year = "2010",

month = feb,

doi = "10.1007/s00011-009-0078-7",

language = "English",

volume = "59",

pages = "123--127",

journal = "Inflammation Research",

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publisher = "Birkhauser Verlag Basel",

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}

TY - JOUR

T1 - Suppression of CXCR4 expression in mast cells upon IgE-mediated antigen stimulation

AU - Matsuura, Junji

AU - Sakanaka, Mariko

AU - Sato, Norio

AU - Ichikawa, Atsushi

AU - Tanaka, Satoshi

N1 - Funding Information: Acknowledgments We thank Dr. A. Popiel for her help in preparation of the manuscript. This study was supported in part by KAKENHI (19590091) Grants-in-Aid for Scientific Research (C) from Japan Society for the Promotion of Science(JSPS).

PY - 2010/2

Y1 - 2010/2

N2 - Objective: Recent studies have demonstrated that a variety of chemokine receptors are expressed in mast cells. We investigated the changes in mRNA expression of CXCRs in murine IL-3-dependent bone marrow-derived mast cells (BMMCs) to clarify how the CXCR expression is regulated in mast cells. Methods: Expression of CXCR mRNA was measured by RNase protection assay. Functional expression of CXCRs was confirmed by monitoring intracellular Ca2+ mobilization. Results: CXCR4 mRNA expression was transiently induced in BMMCs in serum-dependent fashion and was completely suppressed upon IgE-mediated antigen stimulation. In contrast, CXCR5 mRNA expression was induced upon IgE-mediated antigen stimulation. Changes in the intracellular Ca2+ mobilization induced by CXCL12 strongly indicated the functional expression of CXCR4. The decrease in CXCR4 and the increase in CXCR5 mRNA expression was also observed in BMMCs stimulated with thapsigargin, a phorbol ester, and stem cell factor. Conclusion: The mRNA expression of CXCR4 is differentially regulated in BMMCs upon various stimuli including IgE-mediated antigen stimulation.

AB - Objective: Recent studies have demonstrated that a variety of chemokine receptors are expressed in mast cells. We investigated the changes in mRNA expression of CXCRs in murine IL-3-dependent bone marrow-derived mast cells (BMMCs) to clarify how the CXCR expression is regulated in mast cells. Methods: Expression of CXCR mRNA was measured by RNase protection assay. Functional expression of CXCRs was confirmed by monitoring intracellular Ca2+ mobilization. Results: CXCR4 mRNA expression was transiently induced in BMMCs in serum-dependent fashion and was completely suppressed upon IgE-mediated antigen stimulation. In contrast, CXCR5 mRNA expression was induced upon IgE-mediated antigen stimulation. Changes in the intracellular Ca2+ mobilization induced by CXCL12 strongly indicated the functional expression of CXCR4. The decrease in CXCR4 and the increase in CXCR5 mRNA expression was also observed in BMMCs stimulated with thapsigargin, a phorbol ester, and stem cell factor. Conclusion: The mRNA expression of CXCR4 is differentially regulated in BMMCs upon various stimuli including IgE-mediated antigen stimulation.

KW - CXCR4

KW - CXCR5

KW - Chemokine

KW - Mast cell

KW - SCF

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Suppression of CXCR4 expression in mast cells upon IgE-mediated antigen stimulation

Abstract

Keywords

ASJC Scopus subject areas

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