Suppression of Cdc27B expression induces plant defence responses

Chikako Kudo, Tomoko Suzuki, Sumie Fukuoka, Shuta Asai, Hiroko Suenaga, Michiko Sasabe, Yoshitaka Takano, Tetsuro Okuno, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose, Yoshi Shige Inagaki

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Abstract

Non-host resistance is the most general form of disease resistance in plants because it is effective against most phytopathogens. The importance of hypersensitive responses (HRs) in non-host resistance of Nicotiana species to the oomycete Phytophthora is clear. INF1 elicitin, an elicitor obtained from the late-blight pathogen Phytophthora infestans, is sufficient to induce a typical HR in Nicotiana species. The molecular mechanisms that underlie the non-host resistance component of plant defence responses have been investigated using differential-display polymerase chain reaction (PCR) in a model HR system between INF1 elicitin and tobacco BY-2 cells. Differential-display PCR has revealed that Cdc27B is down-regulated in tobacco BY-2 cells after treatment with INF1 elicitin. Cdc27B is one of 13 essential components of the anaphase-promoting complex or cyclosome (APC/C)-type E3 ubiquitin ligase complex in yeast. This APC/C-type E3 ubiquitin ligase complex regulates G2-to-M phase transition of the cell cycle by proteolytic degradation. In this study, we investigated the roles of this gene, NbCdc27B, in plant defence responses using virus-induced gene silencing. Suppression of NbCdc27B in Nicotiana benthamiana plants induced defence responses and a gain of resistance to Colletotrichum lagenarium fungus. Elicitin-induced hypersensitive cell death (HCD) was inhibited mildly in plants silenced with tobacco rattle virus::Cdc27B. Cdc27B could manage the signalling pathways of plant defence responses as a negative regulator without HCD.

Original languageEnglish
Pages (from-to)365-373
Number of pages9
JournalMolecular Plant Pathology
Volume8
Issue number4
DOIs
Publication statusPublished - Jul 2007

Fingerprint

plant defense
tobacco
Tobacco
polymerase chain reaction
virus
hypersensitive response
Anaphase-Promoting Complex-Cyclosome
ubiquitin-protein ligase
Ubiquitin-Protein Ligases
disease resistance
gene
Nicotiana
Phytophthora infestans
phase transition
yeast
cell death
Cell Death
pathogen
fungus
Oomycetes

ASJC Scopus subject areas

  • Plant Science

Cite this

Kudo, C., Suzuki, T., Fukuoka, S., Asai, S., Suenaga, H., Sasabe, M., ... Inagaki, Y. S. (2007). Suppression of Cdc27B expression induces plant defence responses. Molecular Plant Pathology, 8(4), 365-373. https://doi.org/10.1111/j.1364-3703.2007.00397.x

Suppression of Cdc27B expression induces plant defence responses. / Kudo, Chikako; Suzuki, Tomoko; Fukuoka, Sumie; Asai, Shuta; Suenaga, Hiroko; Sasabe, Michiko; Takano, Yoshitaka; Okuno, Tetsuro; Toyoda, Kazuhiro; Shiraishi, Tomonori; Ichinose, Yuki; Inagaki, Yoshi Shige.

In: Molecular Plant Pathology, Vol. 8, No. 4, 07.2007, p. 365-373.

Research output: Contribution to journalArticle

Kudo, C, Suzuki, T, Fukuoka, S, Asai, S, Suenaga, H, Sasabe, M, Takano, Y, Okuno, T, Toyoda, K, Shiraishi, T, Ichinose, Y & Inagaki, YS 2007, 'Suppression of Cdc27B expression induces plant defence responses', Molecular Plant Pathology, vol. 8, no. 4, pp. 365-373. https://doi.org/10.1111/j.1364-3703.2007.00397.x
Kudo, Chikako ; Suzuki, Tomoko ; Fukuoka, Sumie ; Asai, Shuta ; Suenaga, Hiroko ; Sasabe, Michiko ; Takano, Yoshitaka ; Okuno, Tetsuro ; Toyoda, Kazuhiro ; Shiraishi, Tomonori ; Ichinose, Yuki ; Inagaki, Yoshi Shige. / Suppression of Cdc27B expression induces plant defence responses. In: Molecular Plant Pathology. 2007 ; Vol. 8, No. 4. pp. 365-373.
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