Abstract
Non-host resistance is the most general form of disease resistance in plants because it is effective against most phytopathogens. The importance of hypersensitive responses (HRs) in non-host resistance of Nicotiana species to the oomycete Phytophthora is clear. INF1 elicitin, an elicitor obtained from the late-blight pathogen Phytophthora infestans, is sufficient to induce a typical HR in Nicotiana species. The molecular mechanisms that underlie the non-host resistance component of plant defence responses have been investigated using differential-display polymerase chain reaction (PCR) in a model HR system between INF1 elicitin and tobacco BY-2 cells. Differential-display PCR has revealed that Cdc27B is down-regulated in tobacco BY-2 cells after treatment with INF1 elicitin. Cdc27B is one of 13 essential components of the anaphase-promoting complex or cyclosome (APC/C)-type E3 ubiquitin ligase complex in yeast. This APC/C-type E3 ubiquitin ligase complex regulates G2-to-M phase transition of the cell cycle by proteolytic degradation. In this study, we investigated the roles of this gene, NbCdc27B, in plant defence responses using virus-induced gene silencing. Suppression of NbCdc27B in Nicotiana benthamiana plants induced defence responses and a gain of resistance to Colletotrichum lagenarium fungus. Elicitin-induced hypersensitive cell death (HCD) was inhibited mildly in plants silenced with tobacco rattle virus::Cdc27B. Cdc27B could manage the signalling pathways of plant defence responses as a negative regulator without HCD.
| Original language | English |
|---|---|
| Pages (from-to) | 365-373 |
| Number of pages | 9 |
| Journal | Molecular Plant Pathology |
| Volume | 8 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Jul 2007 |
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Suppression of Cdc27B expression induces plant defence responses. / Kudo, Chikako; Suzuki, Tomoko; Fukuoka, Sumie; Asai, Shuta; Suenaga, Hiroko; Sasabe, Michiko; Takano, Yoshitaka; Okuno, Tetsuro; Toyoda, Kazuhiro; Shiraishi, Tomonori; Ichinose, Yuki; Inagaki, Yoshi Shige.
In: Molecular Plant Pathology, Vol. 8, No. 4, 07.2007, p. 365-373.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Suppression of Cdc27B expression induces plant defence responses
AU - Kudo, Chikako
AU - Suzuki, Tomoko
AU - Fukuoka, Sumie
AU - Asai, Shuta
AU - Suenaga, Hiroko
AU - Sasabe, Michiko
AU - Takano, Yoshitaka
AU - Okuno, Tetsuro
AU - Toyoda, Kazuhiro
AU - Shiraishi, Tomonori
AU - Ichinose, Yuki
AU - Inagaki, Yoshi Shige
PY - 2007/7
Y1 - 2007/7
N2 - Non-host resistance is the most general form of disease resistance in plants because it is effective against most phytopathogens. The importance of hypersensitive responses (HRs) in non-host resistance of Nicotiana species to the oomycete Phytophthora is clear. INF1 elicitin, an elicitor obtained from the late-blight pathogen Phytophthora infestans, is sufficient to induce a typical HR in Nicotiana species. The molecular mechanisms that underlie the non-host resistance component of plant defence responses have been investigated using differential-display polymerase chain reaction (PCR) in a model HR system between INF1 elicitin and tobacco BY-2 cells. Differential-display PCR has revealed that Cdc27B is down-regulated in tobacco BY-2 cells after treatment with INF1 elicitin. Cdc27B is one of 13 essential components of the anaphase-promoting complex or cyclosome (APC/C)-type E3 ubiquitin ligase complex in yeast. This APC/C-type E3 ubiquitin ligase complex regulates G2-to-M phase transition of the cell cycle by proteolytic degradation. In this study, we investigated the roles of this gene, NbCdc27B, in plant defence responses using virus-induced gene silencing. Suppression of NbCdc27B in Nicotiana benthamiana plants induced defence responses and a gain of resistance to Colletotrichum lagenarium fungus. Elicitin-induced hypersensitive cell death (HCD) was inhibited mildly in plants silenced with tobacco rattle virus::Cdc27B. Cdc27B could manage the signalling pathways of plant defence responses as a negative regulator without HCD.
AB - Non-host resistance is the most general form of disease resistance in plants because it is effective against most phytopathogens. The importance of hypersensitive responses (HRs) in non-host resistance of Nicotiana species to the oomycete Phytophthora is clear. INF1 elicitin, an elicitor obtained from the late-blight pathogen Phytophthora infestans, is sufficient to induce a typical HR in Nicotiana species. The molecular mechanisms that underlie the non-host resistance component of plant defence responses have been investigated using differential-display polymerase chain reaction (PCR) in a model HR system between INF1 elicitin and tobacco BY-2 cells. Differential-display PCR has revealed that Cdc27B is down-regulated in tobacco BY-2 cells after treatment with INF1 elicitin. Cdc27B is one of 13 essential components of the anaphase-promoting complex or cyclosome (APC/C)-type E3 ubiquitin ligase complex in yeast. This APC/C-type E3 ubiquitin ligase complex regulates G2-to-M phase transition of the cell cycle by proteolytic degradation. In this study, we investigated the roles of this gene, NbCdc27B, in plant defence responses using virus-induced gene silencing. Suppression of NbCdc27B in Nicotiana benthamiana plants induced defence responses and a gain of resistance to Colletotrichum lagenarium fungus. Elicitin-induced hypersensitive cell death (HCD) was inhibited mildly in plants silenced with tobacco rattle virus::Cdc27B. Cdc27B could manage the signalling pathways of plant defence responses as a negative regulator without HCD.
UR - http://www.scopus.com/inward/record.url?scp=34250839509&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34250839509&partnerID=8YFLogxK
U2 - 10.1111/j.1364-3703.2007.00397.x
DO - 10.1111/j.1364-3703.2007.00397.x
M3 - Article
C2 - 20507506
AN - SCOPUS:34250839509
VL - 8
SP - 365
EP - 373
JO - Molecular Plant Pathology
JF - Molecular Plant Pathology
SN - 1464-6722
IS - 4
ER -