Suppression of AP-1 activity by cycloprodigiosin hydrochloride

Keiko Kawauchi, Kei Tobiume, Shuhei Kaneko, Keiko Kaneshiro, Shiho Okamoto, Emi Ueda, Hideaki Kamata, Yoshinori Moriyama, Hajime Hirata

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Cycloprodigiosin hydrochloride (cPrG · HCl), a compound isolated from a marine bacterium, acts as an immunosuppressant and an anti-cancer drug. We have previously reported that cPrG · HCl suppressed the transcriptional activation of nuclear factor (NF)-κB. Here we studied the effect of cPrG · HCl on activation of another transcription factor, activator protein 1 (AP-1). cPrG · HCl potently suppressed AP-1 activity induced by tumor necrosis factor (TNF) α and phorbol myristate acetate (PMA). cPrG · HCl did not inhibit any of the mitogen-activated protein kinase (MAPK) families, whereas it did suppress transcriptional activation of AP-1 induced by constitutively activated mutants of MEKK1 or Ras. cPrG · HCl inhibited neither TNFα- or PMA-induced DNA-binding of AP-1 nor co-activator p300-induced activation of AP-1. Taken together, cPrG · HCl suppresses AP-1-dependent gene expression downstream of MAPK group through the inhibition of the transcription activation step of the AP-1 promoter complex.

Original languageEnglish
Pages (from-to)1792-1795
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume30
Issue number9
DOIs
Publication statusPublished - Sep 2007

Keywords

  • Activator protein 1
  • Mitogen-activated protein kinase
  • Nuclear factor (NF)-κB
  • Prodigiosin

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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