Abstract
The host's immune system discriminates tumor cells from normal cells by recognizing the major histocompatibility complex (MHC) class I antigen expressed on the tumor cell membrane. However, the role of MHC class I antigen in tumor cells has not yet been clarified. In this study, the influence of MHC class I antigen expression on the tumorigenicity of a human glioblastoma cell line (KMG4) is examined. Barely detectable levels of MHC class I messenger ribonucleic acid were found to express in KMG4 cells by Northern blot analysis using mouse MHC class I (H-2L(d)) and human leukocyte antigen (HLA)-B7 genes as probes. The H-2L(d) gene connected at the downstream end of murine mammary tumor virus (MMTV)-promoter was cotransfected with the neomycine-resistant gene pSV2-neo into KMG4 cells, and the drug-resistant cells were selected. The KMG4 cells (KMG4-MMTV-L(d)), which acquired the MHC class I gene were detected by Northern blot analysis with H-2L(d) as the probe, and by immunohistochemistry using the H-2L(d)- specific monoclonal antibody. Tumorigenicity, as determined by colony-forming ability in soft agar, was then compared between MHC class I-expressing KMG4- MMTV-L(d) and nonexpressing control cells. The MHC class I-expressing cells were found to be deprived of colony-forming ability, indicating that MHC class I antigen could negatively influence the anchorage-independent cell growth of the human glioblastoma cell line KMG4.
| Original language | English |
|---|---|
| Pages (from-to) | 845-849 |
| Number of pages | 5 |
| Journal | Journal of Neurosurgery |
| Volume | 76 |
| Issue number | 5 |
| Publication status | Published - 1992 |
| Externally published | Yes |
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Keywords
- antigen
- brain neoplasm
- glioblastoma
- major histocompatibility complex
- signal transduction
- tumor growth
ASJC Scopus subject areas
- Clinical Neurology
- Neuroscience(all)
Cite this
Suppression of anchorage-independent growth of human glioblastoma cell by major histocompatibility complex class I gene-transfection. / Momozaki, N.; Oouchida, Mamoru; Tabuchi, K.; Ikezaki, K.; Hori, K.
In: Journal of Neurosurgery, Vol. 76, No. 5, 1992, p. 845-849.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Suppression of anchorage-independent growth of human glioblastoma cell by major histocompatibility complex class I gene-transfection
AU - Momozaki, N.
AU - Oouchida, Mamoru
AU - Tabuchi, K.
AU - Ikezaki, K.
AU - Hori, K.
PY - 1992
Y1 - 1992
N2 - The host's immune system discriminates tumor cells from normal cells by recognizing the major histocompatibility complex (MHC) class I antigen expressed on the tumor cell membrane. However, the role of MHC class I antigen in tumor cells has not yet been clarified. In this study, the influence of MHC class I antigen expression on the tumorigenicity of a human glioblastoma cell line (KMG4) is examined. Barely detectable levels of MHC class I messenger ribonucleic acid were found to express in KMG4 cells by Northern blot analysis using mouse MHC class I (H-2L(d)) and human leukocyte antigen (HLA)-B7 genes as probes. The H-2L(d) gene connected at the downstream end of murine mammary tumor virus (MMTV)-promoter was cotransfected with the neomycine-resistant gene pSV2-neo into KMG4 cells, and the drug-resistant cells were selected. The KMG4 cells (KMG4-MMTV-L(d)), which acquired the MHC class I gene were detected by Northern blot analysis with H-2L(d) as the probe, and by immunohistochemistry using the H-2L(d)- specific monoclonal antibody. Tumorigenicity, as determined by colony-forming ability in soft agar, was then compared between MHC class I-expressing KMG4- MMTV-L(d) and nonexpressing control cells. The MHC class I-expressing cells were found to be deprived of colony-forming ability, indicating that MHC class I antigen could negatively influence the anchorage-independent cell growth of the human glioblastoma cell line KMG4.
AB - The host's immune system discriminates tumor cells from normal cells by recognizing the major histocompatibility complex (MHC) class I antigen expressed on the tumor cell membrane. However, the role of MHC class I antigen in tumor cells has not yet been clarified. In this study, the influence of MHC class I antigen expression on the tumorigenicity of a human glioblastoma cell line (KMG4) is examined. Barely detectable levels of MHC class I messenger ribonucleic acid were found to express in KMG4 cells by Northern blot analysis using mouse MHC class I (H-2L(d)) and human leukocyte antigen (HLA)-B7 genes as probes. The H-2L(d) gene connected at the downstream end of murine mammary tumor virus (MMTV)-promoter was cotransfected with the neomycine-resistant gene pSV2-neo into KMG4 cells, and the drug-resistant cells were selected. The KMG4 cells (KMG4-MMTV-L(d)), which acquired the MHC class I gene were detected by Northern blot analysis with H-2L(d) as the probe, and by immunohistochemistry using the H-2L(d)- specific monoclonal antibody. Tumorigenicity, as determined by colony-forming ability in soft agar, was then compared between MHC class I-expressing KMG4- MMTV-L(d) and nonexpressing control cells. The MHC class I-expressing cells were found to be deprived of colony-forming ability, indicating that MHC class I antigen could negatively influence the anchorage-independent cell growth of the human glioblastoma cell line KMG4.
KW - antigen
KW - brain neoplasm
KW - glioblastoma
KW - major histocompatibility complex
KW - signal transduction
KW - tumor growth
UR - http://www.scopus.com/inward/record.url?scp=0026537426&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026537426&partnerID=8YFLogxK
M3 - Article
C2 - 1564545
AN - SCOPUS:0026537426
VL - 76
SP - 845
EP - 849
JO - Journal of Neurosurgery
JF - Journal of Neurosurgery
SN - 0022-3085
IS - 5
ER -