Suppression of anchorage-independent growth of human glioblastoma cell by major histocompatibility complex class I gene-transfection

N. Momozaki, Mamoru Oouchida, K. Tabuchi, K. Ikezaki, K. Hori

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The host's immune system discriminates tumor cells from normal cells by recognizing the major histocompatibility complex (MHC) class I antigen expressed on the tumor cell membrane. However, the role of MHC class I antigen in tumor cells has not yet been clarified. In this study, the influence of MHC class I antigen expression on the tumorigenicity of a human glioblastoma cell line (KMG4) is examined. Barely detectable levels of MHC class I messenger ribonucleic acid were found to express in KMG4 cells by Northern blot analysis using mouse MHC class I (H-2L(d)) and human leukocyte antigen (HLA)-B7 genes as probes. The H-2L(d) gene connected at the downstream end of murine mammary tumor virus (MMTV)-promoter was cotransfected with the neomycine-resistant gene pSV2-neo into KMG4 cells, and the drug-resistant cells were selected. The KMG4 cells (KMG4-MMTV-L(d)), which acquired the MHC class I gene were detected by Northern blot analysis with H-2L(d) as the probe, and by immunohistochemistry using the H-2L(d)- specific monoclonal antibody. Tumorigenicity, as determined by colony-forming ability in soft agar, was then compared between MHC class I-expressing KMG4- MMTV-L(d) and nonexpressing control cells. The MHC class I-expressing cells were found to be deprived of colony-forming ability, indicating that MHC class I antigen could negatively influence the anchorage-independent cell growth of the human glioblastoma cell line KMG4.

Original languageEnglish
Pages (from-to)845-849
Number of pages5
JournalJournal of Neurosurgery
Volume76
Issue number5
Publication statusPublished - 1992
Externally publishedYes

Fingerprint

MHC Class I Genes
Glioblastoma
Major Histocompatibility Complex
Transfection
Growth
Histocompatibility Antigens Class I
Gammaretrovirus
Breast Neoplasms
Northern Blotting
Genes
Cell Line
Neoplasms
HLA Antigens
Carcinogens
Agar
Immune System
Immunohistochemistry
Monoclonal Antibodies
Cell Membrane
RNA

Keywords

  • antigen
  • brain neoplasm
  • glioblastoma
  • major histocompatibility complex
  • signal transduction
  • tumor growth

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Suppression of anchorage-independent growth of human glioblastoma cell by major histocompatibility complex class I gene-transfection. / Momozaki, N.; Oouchida, Mamoru; Tabuchi, K.; Ikezaki, K.; Hori, K.

In: Journal of Neurosurgery, Vol. 76, No. 5, 1992, p. 845-849.

Research output: Contribution to journalArticle

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