Suppression of adiponectin by aberrantly glycosylated IgA1 in glomerular mesangial cells in vitro and in vivo

Tatsuyuki Inoue, Hitoshi Sugiyama, Masashi Kitagawa, Keiichi Takiue, Hiroshi Morinaga, Ayu Ogawa, Yoko Kikumoto, Shinji Kitamura, Yohei Maeshima, Hirofumi Makino

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Abstract

The pathogenesis of IgA nephropathy (IgAN) may be associated with the mesangial deposition of aberrantly glycosylated IgA1. To identify mediators affected by aberrantly glycosylated IgA1 in cultured human mesangial cells (HMCs), we generated enzymatically modified desialylated and degalactosylated (deSial/deGal) IgA1. The state of deglycosylated IgA1 was confirmed by lectin binding to Helix aspersa (HAA) and Sambucus nigra (SNA). In the cytokine array analysis, 52 proteins were upregulated and 34 were downregulated in HMCs after stimulation with deSial/deGal IgA1. Among them, the secretion of adiponectin was suppressed in HMCs after stimulation with deSial/deGal IgA1. HMCs expressed mRNAs for adiponectin and its type 1 receptor, but not the type 2 receptor. Moreover, we revealed a downregulation of adiponectin expression in the glomeruli of renal biopsy specimens from patients with IgAN compared to those with lupus nephritis. We also demonstrated that aberrantly glycosylated IgA1 was deposited in the mesangium of patients with IgAN by dual staining of HAA and IgA. Moreover, the urinary HAA/SNA ratio of lectin binding was significantly higher in IgAN compared to other kidney diseases. Since adiponectin has anti-inflammatory effects, including the inhibition of adhesion molecules and cytokines, these data suggest that the local suppression of this adipokine by aberrantly glycosylated IgA1 could be involved in the regulation of glomerular inflammation and sclerosis in IgAN.

Original languageEnglish
Article numbere33965
JournalPLoS One
Volume7
Issue number3
DOIs
Publication statusPublished - Mar 23 2012

Fingerprint

adiponectin
Mesangial Cells
Adiponectin
kidney diseases
Immunoglobulin A
Helix aspersa
Sambucus nigra
cells
lectins
cytokines
adipokines
receptors
nephritis
sclerosis
anti-inflammatory activity
adhesion
In Vitro Techniques
biopsy
Lectins
pathogenesis

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Suppression of adiponectin by aberrantly glycosylated IgA1 in glomerular mesangial cells in vitro and in vivo. / Inoue, Tatsuyuki; Sugiyama, Hitoshi; Kitagawa, Masashi; Takiue, Keiichi; Morinaga, Hiroshi; Ogawa, Ayu; Kikumoto, Yoko; Kitamura, Shinji; Maeshima, Yohei; Makino, Hirofumi.

In: PLoS One, Vol. 7, No. 3, e33965, 23.03.2012.

Research output: Contribution to journalArticle

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