Suppressed apoptotic induction in esophageal squamous cell carcinomas expressing extensive p53 protein

Madoka Hamada, Yoshio Naomoto, Toshiyoshi Fujiwara, Yasuaki Kamikawa, Noriaki Tanaka

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

p53 protein accumulation, thought to be caused by p53 gene mutation, is closely related to poor prognosis of patients with certain types of carcinomas. The progression of esophageal squamous cell carcinoma (SCC) is also strongly suspected to depend on the p53 tumor suppressor gene. Formalin-fixed, paraffin-embedded sections were taken from 25 patients who underwent esophagectomy for SCC. Fourteen patients had no preoperative therapy (control group), while the other 11 patients received preoperative radiotherapy (radiation group). There was no difference in pathological TNM classification between the two groups. These sections were examined by immunostaining with monoclonal antibody PAb 1801 to determine the accumulation of p53 protein , and apoptotic frequency was determined by TdT mediated dUTP-biotin nick end-labeling (TUNEL). In the control group, well to moderately differentiated cases showed a significantly higher Al (apoptotic index which is the number of apoptotic cells among 1000 cancer cells. ‰) (51.7±83.4) than poorly differentiated cases (Al = 1.3±1.0) (P

Original languageEnglish
Pages (from-to)398-404
Number of pages7
JournalJapanese Journal of Clinical Oncology
Volume26
Issue number6
Publication statusPublished - Dec 1996

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Proteins
Control Groups
Esophagectomy
Neoplasm Staging
p53 Genes
Biotin
Tumor Suppressor Genes
Paraffin
Formaldehyde
Squamous Cell Carcinoma
Radiotherapy
Cell Count
Monoclonal Antibodies
Radiation
Carcinoma
Mutation
Esophageal Squamous Cell Carcinoma
Neoplasms
Therapeutics

Keywords

  • Apoptosis
  • Esophagus
  • Gene therapy
  • p53
  • Squamous cell carcinoma

ASJC Scopus subject areas

  • Oncology

Cite this

Suppressed apoptotic induction in esophageal squamous cell carcinomas expressing extensive p53 protein. / Hamada, Madoka; Naomoto, Yoshio; Fujiwara, Toshiyoshi; Kamikawa, Yasuaki; Tanaka, Noriaki.

In: Japanese Journal of Clinical Oncology, Vol. 26, No. 6, 12.1996, p. 398-404.

Research output: Contribution to journalArticle

Hamada, Madoka ; Naomoto, Yoshio ; Fujiwara, Toshiyoshi ; Kamikawa, Yasuaki ; Tanaka, Noriaki. / Suppressed apoptotic induction in esophageal squamous cell carcinomas expressing extensive p53 protein. In: Japanese Journal of Clinical Oncology. 1996 ; Vol. 26, No. 6. pp. 398-404.
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