Supplementation of parenteral nutrition with fish oil attenuates acute lung injury in a rat model

Keisuke Kohama, Atsunori Nakao, Mariko Terashima, Michiko Aoyama-Ishikawa, Takayuki Shimizu, Daisuke Harada, Mitsuo Nakayama, Hayato Yamashita, Mayu Fujiwara, Joji Kotani

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Fish oil rich in n-3 polyunsaturated fatty acids has diverse immunomodulatory properties and attenuates acute lung injury when administered in enternal nutrition. However, enteral nutrition is not always feasible. Therefore, we investigated the ability of parenteral nutrition supplemented with fish oil to ameliorate acute lung injury. Rats were infused with parenteral nutrition solutions (without lipids, with soybean oil, or with soybean oil and fish oil) for three days. Lipopolysaccharide (15 mg/kg) was then administered intratracheally to induce acute lung injury, characterized by impaired lung function, polymorphonuclear leukocyte recruitment, parenchymal tissue damage, and upregulation of mRNAs for inflammatory mediators. Administration of parenteral nutrition supplemented with fish oil prior to lung insult improved gas exchange and inhibited neutrophil recruitment and upregulation of mRNAs for inflammatory mediators. Parenteral nutrition supplemented with fish oil also prolonged survival. To investigate the underlying mechanisms, leukotriene B 4 and leukotriene B5 secretion was measured in neutrophils from the peritoneal cavity. The neutrophils from rats treated with fish oil-rich parenteral nutrition released significantly more leukotriene B 5, an antiinflammatory eicosanoid, than neutrophils isolated from rats given standard parenteral nutrition. Parenteral nutrition with fish oil significantly reduced lipopolysaccharide-induced lung injury in rats in part by promoting the synthesis of anti-inflammatory eicosanoids.

Original languageEnglish
Pages (from-to)116-121
Number of pages6
JournalJournal of Clinical Biochemistry and Nutrition
Volume54
Issue number2
DOIs
Publication statusPublished - Mar 1 2014
Externally publishedYes

Fingerprint

Fish Oils
Acute Lung Injury
Parenteral Nutrition
Nutrition
Rats
Neutrophils
Soybean Oil
Leukotriene B4
Eicosanoids
Lipopolysaccharides
Anti-Inflammatory Agents
Up-Regulation
Parenteral Nutrition Solutions
Lung
Messenger RNA
Neutrophil Infiltration
Peritoneal Cavity
Omega-3 Fatty Acids
Enteral Nutrition
Lung Injury

Keywords

  • Acute lung injury
  • Fish oil
  • Nutritional support
  • Omega-3 fatty acids
  • Rat model

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Supplementation of parenteral nutrition with fish oil attenuates acute lung injury in a rat model. / Kohama, Keisuke; Nakao, Atsunori; Terashima, Mariko; Aoyama-Ishikawa, Michiko; Shimizu, Takayuki; Harada, Daisuke; Nakayama, Mitsuo; Yamashita, Hayato; Fujiwara, Mayu; Kotani, Joji.

In: Journal of Clinical Biochemistry and Nutrition, Vol. 54, No. 2, 01.03.2014, p. 116-121.

Research output: Contribution to journalArticle

Kohama, K, Nakao, A, Terashima, M, Aoyama-Ishikawa, M, Shimizu, T, Harada, D, Nakayama, M, Yamashita, H, Fujiwara, M & Kotani, J 2014, 'Supplementation of parenteral nutrition with fish oil attenuates acute lung injury in a rat model', Journal of Clinical Biochemistry and Nutrition, vol. 54, no. 2, pp. 116-121. https://doi.org/10.3164/jcbn.13-90
Kohama, Keisuke ; Nakao, Atsunori ; Terashima, Mariko ; Aoyama-Ishikawa, Michiko ; Shimizu, Takayuki ; Harada, Daisuke ; Nakayama, Mitsuo ; Yamashita, Hayato ; Fujiwara, Mayu ; Kotani, Joji. / Supplementation of parenteral nutrition with fish oil attenuates acute lung injury in a rat model. In: Journal of Clinical Biochemistry and Nutrition. 2014 ; Vol. 54, No. 2. pp. 116-121.
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