Abstract
Fish oil rich in n-3 polyunsaturated fatty acids has diverse immunomodulatory properties and attenuates acute lung injury when administered in enternal nutrition. However, enteral nutrition is not always feasible. Therefore, we investigated the ability of parenteral nutrition supplemented with fish oil to ameliorate acute lung injury. Rats were infused with parenteral nutrition solutions (without lipids, with soybean oil, or with soybean oil and fish oil) for three days. Lipopolysaccharide (15 mg/kg) was then administered intratracheally to induce acute lung injury, characterized by impaired lung function, polymorphonuclear leukocyte recruitment, parenchymal tissue damage, and upregulation of mRNAs for inflammatory mediators. Administration of parenteral nutrition supplemented with fish oil prior to lung insult improved gas exchange and inhibited neutrophil recruitment and upregulation of mRNAs for inflammatory mediators. Parenteral nutrition supplemented with fish oil also prolonged survival. To investigate the underlying mechanisms, leukotriene B 4 and leukotriene B5 secretion was measured in neutrophils from the peritoneal cavity. The neutrophils from rats treated with fish oil-rich parenteral nutrition released significantly more leukotriene B 5, an antiinflammatory eicosanoid, than neutrophils isolated from rats given standard parenteral nutrition. Parenteral nutrition with fish oil significantly reduced lipopolysaccharide-induced lung injury in rats in part by promoting the synthesis of anti-inflammatory eicosanoids.
| Original language | English |
|---|---|
| Pages (from-to) | 116-121 |
| Number of pages | 6 |
| Journal | Journal of Clinical Biochemistry and Nutrition |
| Volume | 54 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Mar 1 2014 |
| Externally published | Yes |
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Keywords
- Acute lung injury
- Fish oil
- Nutritional support
- Omega-3 fatty acids
- Rat model
ASJC Scopus subject areas
- Clinical Biochemistry
- Medicine (miscellaneous)
- Nutrition and Dietetics
Cite this
Supplementation of parenteral nutrition with fish oil attenuates acute lung injury in a rat model. / Kohama, Keisuke; Nakao, Atsunori; Terashima, Mariko; Aoyama-Ishikawa, Michiko; Shimizu, Takayuki; Harada, Daisuke; Nakayama, Mitsuo; Yamashita, Hayato; Fujiwara, Mayu; Kotani, Joji.
In: Journal of Clinical Biochemistry and Nutrition, Vol. 54, No. 2, 01.03.2014, p. 116-121.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Supplementation of parenteral nutrition with fish oil attenuates acute lung injury in a rat model
AU - Kohama, Keisuke
AU - Nakao, Atsunori
AU - Terashima, Mariko
AU - Aoyama-Ishikawa, Michiko
AU - Shimizu, Takayuki
AU - Harada, Daisuke
AU - Nakayama, Mitsuo
AU - Yamashita, Hayato
AU - Fujiwara, Mayu
AU - Kotani, Joji
PY - 2014/3/1
Y1 - 2014/3/1
N2 - Fish oil rich in n-3 polyunsaturated fatty acids has diverse immunomodulatory properties and attenuates acute lung injury when administered in enternal nutrition. However, enteral nutrition is not always feasible. Therefore, we investigated the ability of parenteral nutrition supplemented with fish oil to ameliorate acute lung injury. Rats were infused with parenteral nutrition solutions (without lipids, with soybean oil, or with soybean oil and fish oil) for three days. Lipopolysaccharide (15 mg/kg) was then administered intratracheally to induce acute lung injury, characterized by impaired lung function, polymorphonuclear leukocyte recruitment, parenchymal tissue damage, and upregulation of mRNAs for inflammatory mediators. Administration of parenteral nutrition supplemented with fish oil prior to lung insult improved gas exchange and inhibited neutrophil recruitment and upregulation of mRNAs for inflammatory mediators. Parenteral nutrition supplemented with fish oil also prolonged survival. To investigate the underlying mechanisms, leukotriene B 4 and leukotriene B5 secretion was measured in neutrophils from the peritoneal cavity. The neutrophils from rats treated with fish oil-rich parenteral nutrition released significantly more leukotriene B 5, an antiinflammatory eicosanoid, than neutrophils isolated from rats given standard parenteral nutrition. Parenteral nutrition with fish oil significantly reduced lipopolysaccharide-induced lung injury in rats in part by promoting the synthesis of anti-inflammatory eicosanoids.
AB - Fish oil rich in n-3 polyunsaturated fatty acids has diverse immunomodulatory properties and attenuates acute lung injury when administered in enternal nutrition. However, enteral nutrition is not always feasible. Therefore, we investigated the ability of parenteral nutrition supplemented with fish oil to ameliorate acute lung injury. Rats were infused with parenteral nutrition solutions (without lipids, with soybean oil, or with soybean oil and fish oil) for three days. Lipopolysaccharide (15 mg/kg) was then administered intratracheally to induce acute lung injury, characterized by impaired lung function, polymorphonuclear leukocyte recruitment, parenchymal tissue damage, and upregulation of mRNAs for inflammatory mediators. Administration of parenteral nutrition supplemented with fish oil prior to lung insult improved gas exchange and inhibited neutrophil recruitment and upregulation of mRNAs for inflammatory mediators. Parenteral nutrition supplemented with fish oil also prolonged survival. To investigate the underlying mechanisms, leukotriene B 4 and leukotriene B5 secretion was measured in neutrophils from the peritoneal cavity. The neutrophils from rats treated with fish oil-rich parenteral nutrition released significantly more leukotriene B 5, an antiinflammatory eicosanoid, than neutrophils isolated from rats given standard parenteral nutrition. Parenteral nutrition with fish oil significantly reduced lipopolysaccharide-induced lung injury in rats in part by promoting the synthesis of anti-inflammatory eicosanoids.
KW - Acute lung injury
KW - Fish oil
KW - Nutritional support
KW - Omega-3 fatty acids
KW - Rat model
UR - http://www.scopus.com/inward/record.url?scp=84897509776&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84897509776&partnerID=8YFLogxK
U2 - 10.3164/jcbn.13-90
DO - 10.3164/jcbn.13-90
M3 - Article
AN - SCOPUS:84897509776
VL - 54
SP - 116
EP - 121
JO - Journal of Clinical Biochemistry and Nutrition
JF - Journal of Clinical Biochemistry and Nutrition
SN - 0912-0009
IS - 2
ER -