Supplementation of broccoli or Bifidobacterium longum-fermented broccoli suppresses serum lipid peroxidation and osteoclast differentiation on alveolar bone surface in rats fed a high-cholesterol diet

Takaaki Tomofuji, Daisuke Ekuni, Tetsuji Azuma, Koichiro Irie, Yasumasa Endo, Tatsuo Yamamoto, Atsushi Ishikado, Takehiko Sato, Kayo Harada, Hirohisa Suido, Manabu Morita

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

High-cholesterol diet enhances osteoclastic activity on alveolar bone by increasing serum lipid peroxidation. We hypothesized that supplementation with dietary antioxidants, such as found in broccoli and its fermented products, might suppress increases in serum lipid peroxidation, contributing to the inhibition of osteoclastic activity after high-cholesterol diet intake. The purpose of the present study was to investigate the effects of broccoli and fermented broccoli consumption on serum lipid peroxidation and osteoclast differentiation in alveolar bone of rats fed a high-cholesterol diet. In this 12-week study, rats were divided into 4 groups (n = 6/group): a control group (fed regular diet) and 3 experimental groups (fed a high-cholesterol [1% wt/wt] diet, or a high-cholesterol diet supplemented with either broccoli powder [5% wt/wt] or Bifidobacterium longum-fermented broccoli powder [5% wt/wt]). Serum hexanoyl-lysine (HEL) levels were measured as a parameter of lipid peroxidation. The number of tartrate-resistant acid phosphatase (TRAP)-positive osteoclasts in alveolar bone was enumerated to evaluate osteoclast differentiation. When compared with regular diet, the high-cholesterol diet increased serum HEL levels and resulted in a higher number of TRAP-positive osteoclasts at 12 weeks. The high-cholesterol diet supplemented with broccoli or B. longum-fermented broccoli showed lower levels of serum HEL and fewer TRAP-positive osteoclasts than the high-cholesterol diet at 12 weeks. In conclusion, consumption of broccoli, or its fermented product, inhibited the effects of a high-cholesterol diet on osteoclast differentiation in rat alveolar bone by suppressing serum lipid peroxidation.

Original languageEnglish
Pages (from-to)301-307
Number of pages7
JournalNutrition Research
Volume32
Issue number4
DOIs
Publication statusPublished - Apr 2012

Fingerprint

Brassica
Osteoclasts
Lipid Peroxidation
Cholesterol
Diet
Bone and Bones
Serum
Lysine
Powders
Bifidobacterium longum
Dietary Supplements
Antioxidants

Keywords

  • Alveolar bone loss
  • Broccoli
  • Dietary cholesterol
  • Fermentation
  • Lipid peroxidation
  • Rats

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Nutrition and Dietetics

Cite this

Supplementation of broccoli or Bifidobacterium longum-fermented broccoli suppresses serum lipid peroxidation and osteoclast differentiation on alveolar bone surface in rats fed a high-cholesterol diet. / Tomofuji, Takaaki; Ekuni, Daisuke; Azuma, Tetsuji; Irie, Koichiro; Endo, Yasumasa; Yamamoto, Tatsuo; Ishikado, Atsushi; Sato, Takehiko; Harada, Kayo; Suido, Hirohisa; Morita, Manabu.

In: Nutrition Research, Vol. 32, No. 4, 04.2012, p. 301-307.

Research output: Contribution to journalArticle

Tomofuji, Takaaki ; Ekuni, Daisuke ; Azuma, Tetsuji ; Irie, Koichiro ; Endo, Yasumasa ; Yamamoto, Tatsuo ; Ishikado, Atsushi ; Sato, Takehiko ; Harada, Kayo ; Suido, Hirohisa ; Morita, Manabu. / Supplementation of broccoli or Bifidobacterium longum-fermented broccoli suppresses serum lipid peroxidation and osteoclast differentiation on alveolar bone surface in rats fed a high-cholesterol diet. In: Nutrition Research. 2012 ; Vol. 32, No. 4. pp. 301-307.
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abstract = "High-cholesterol diet enhances osteoclastic activity on alveolar bone by increasing serum lipid peroxidation. We hypothesized that supplementation with dietary antioxidants, such as found in broccoli and its fermented products, might suppress increases in serum lipid peroxidation, contributing to the inhibition of osteoclastic activity after high-cholesterol diet intake. The purpose of the present study was to investigate the effects of broccoli and fermented broccoli consumption on serum lipid peroxidation and osteoclast differentiation in alveolar bone of rats fed a high-cholesterol diet. In this 12-week study, rats were divided into 4 groups (n = 6/group): a control group (fed regular diet) and 3 experimental groups (fed a high-cholesterol [1{\%} wt/wt] diet, or a high-cholesterol diet supplemented with either broccoli powder [5{\%} wt/wt] or Bifidobacterium longum-fermented broccoli powder [5{\%} wt/wt]). Serum hexanoyl-lysine (HEL) levels were measured as a parameter of lipid peroxidation. The number of tartrate-resistant acid phosphatase (TRAP)-positive osteoclasts in alveolar bone was enumerated to evaluate osteoclast differentiation. When compared with regular diet, the high-cholesterol diet increased serum HEL levels and resulted in a higher number of TRAP-positive osteoclasts at 12 weeks. The high-cholesterol diet supplemented with broccoli or B. longum-fermented broccoli showed lower levels of serum HEL and fewer TRAP-positive osteoclasts than the high-cholesterol diet at 12 weeks. In conclusion, consumption of broccoli, or its fermented product, inhibited the effects of a high-cholesterol diet on osteoclast differentiation in rat alveolar bone by suppressing serum lipid peroxidation.",
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AU - Azuma, Tetsuji

AU - Irie, Koichiro

AU - Endo, Yasumasa

AU - Yamamoto, Tatsuo

AU - Ishikado, Atsushi

AU - Sato, Takehiko

AU - Harada, Kayo

AU - Suido, Hirohisa

AU - Morita, Manabu

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KW - Broccoli

KW - Dietary cholesterol

KW - Fermentation

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