Superoxide generation in extracts from isolated plant cell walls is regulated by fungal signal molecules

Akinori Kiba, Chizu Miyake, Kazuhiro Toyoda, Yuki Ichinose, Tetsuji Yamada, Tomonori Shiraishi

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Fractions solubilized with NaCl from cell walls of pea and cowpea plants catalyzed the formation of blue formazan from nitroblue tetrazolium. Because superoxide dismutase decreased formazan production by over 90%, superoxide onion (O2-) may participate in the formation of formazan in the solubilized cell wall fractions. The formazan formation in the fractions solubilized from pea and cowpea cell walls was markedly reduced by exclusion of NAD(P)H, manganese ion, or p-coumaric acid from the reaction mixture. The formazan formation was severely inhibited by salicylhydroxamic acid and catalase, but not by imidazole, pyridine, quinacrine, and diphenyleneiodonium. An elicitor preparation from the pea pathogen Mycosphaerella pinodes enhanced the activities of formazan formation nonspecifically in both pea and cowpea fractions. The suppressor preparation from M. pinodes inhibited the activity in the pea fraction in the presence or absence of the elicitor. In the cowpea fraction, however, the suppressor did not inhibit the elicitor-enhanced activity, and the suppressor alone stimulated formazan formation. These results indicated that O2- generation in the fractions solubilized from pea and cowpea cell walls seems to be catalyzed by cell wall-bound peroxidase(s) and that the plant cell walls alone are able to respond to the elicitor nonspecifically and to the suppressor in a species-specific manner, suggesting the plant cell walls may play an important role in determination of plant-fungal pathogen specificity.

Original languageEnglish
Pages (from-to)846-852
Number of pages7
JournalPhytopathology
Volume87
Issue number8
Publication statusPublished - Aug 1997

Fingerprint

superoxide anion
cell walls
peas
cowpeas
extracts
Mycosphaerella pinodes
quinacrine
nitroblue tetrazolium
imidazoles
pathogens
pyridines
p-coumaric acid
onions
manganese
catalase
superoxide dismutase
peroxidase
ions
elicitors
acids

Keywords

  • Pisum sativum
  • Species specificity
  • Vigna sinensis

ASJC Scopus subject areas

  • Plant Science

Cite this

Superoxide generation in extracts from isolated plant cell walls is regulated by fungal signal molecules. / Kiba, Akinori; Miyake, Chizu; Toyoda, Kazuhiro; Ichinose, Yuki; Yamada, Tetsuji; Shiraishi, Tomonori.

In: Phytopathology, Vol. 87, No. 8, 08.1997, p. 846-852.

Research output: Contribution to journalArticle

Kiba, Akinori ; Miyake, Chizu ; Toyoda, Kazuhiro ; Ichinose, Yuki ; Yamada, Tetsuji ; Shiraishi, Tomonori. / Superoxide generation in extracts from isolated plant cell walls is regulated by fungal signal molecules. In: Phytopathology. 1997 ; Vol. 87, No. 8. pp. 846-852.
@article{e4e1a2f395314222a492b2d0792259fd,
title = "Superoxide generation in extracts from isolated plant cell walls is regulated by fungal signal molecules",
abstract = "Fractions solubilized with NaCl from cell walls of pea and cowpea plants catalyzed the formation of blue formazan from nitroblue tetrazolium. Because superoxide dismutase decreased formazan production by over 90{\%}, superoxide onion (O2-) may participate in the formation of formazan in the solubilized cell wall fractions. The formazan formation in the fractions solubilized from pea and cowpea cell walls was markedly reduced by exclusion of NAD(P)H, manganese ion, or p-coumaric acid from the reaction mixture. The formazan formation was severely inhibited by salicylhydroxamic acid and catalase, but not by imidazole, pyridine, quinacrine, and diphenyleneiodonium. An elicitor preparation from the pea pathogen Mycosphaerella pinodes enhanced the activities of formazan formation nonspecifically in both pea and cowpea fractions. The suppressor preparation from M. pinodes inhibited the activity in the pea fraction in the presence or absence of the elicitor. In the cowpea fraction, however, the suppressor did not inhibit the elicitor-enhanced activity, and the suppressor alone stimulated formazan formation. These results indicated that O2- generation in the fractions solubilized from pea and cowpea cell walls seems to be catalyzed by cell wall-bound peroxidase(s) and that the plant cell walls alone are able to respond to the elicitor nonspecifically and to the suppressor in a species-specific manner, suggesting the plant cell walls may play an important role in determination of plant-fungal pathogen specificity.",
keywords = "Pisum sativum, Species specificity, Vigna sinensis",
author = "Akinori Kiba and Chizu Miyake and Kazuhiro Toyoda and Yuki Ichinose and Tetsuji Yamada and Tomonori Shiraishi",
year = "1997",
month = "8",
language = "English",
volume = "87",
pages = "846--852",
journal = "Phytopathology",
issn = "0031-949X",
publisher = "American Phytopathological Society",
number = "8",

}

TY - JOUR

T1 - Superoxide generation in extracts from isolated plant cell walls is regulated by fungal signal molecules

AU - Kiba, Akinori

AU - Miyake, Chizu

AU - Toyoda, Kazuhiro

AU - Ichinose, Yuki

AU - Yamada, Tetsuji

AU - Shiraishi, Tomonori

PY - 1997/8

Y1 - 1997/8

N2 - Fractions solubilized with NaCl from cell walls of pea and cowpea plants catalyzed the formation of blue formazan from nitroblue tetrazolium. Because superoxide dismutase decreased formazan production by over 90%, superoxide onion (O2-) may participate in the formation of formazan in the solubilized cell wall fractions. The formazan formation in the fractions solubilized from pea and cowpea cell walls was markedly reduced by exclusion of NAD(P)H, manganese ion, or p-coumaric acid from the reaction mixture. The formazan formation was severely inhibited by salicylhydroxamic acid and catalase, but not by imidazole, pyridine, quinacrine, and diphenyleneiodonium. An elicitor preparation from the pea pathogen Mycosphaerella pinodes enhanced the activities of formazan formation nonspecifically in both pea and cowpea fractions. The suppressor preparation from M. pinodes inhibited the activity in the pea fraction in the presence or absence of the elicitor. In the cowpea fraction, however, the suppressor did not inhibit the elicitor-enhanced activity, and the suppressor alone stimulated formazan formation. These results indicated that O2- generation in the fractions solubilized from pea and cowpea cell walls seems to be catalyzed by cell wall-bound peroxidase(s) and that the plant cell walls alone are able to respond to the elicitor nonspecifically and to the suppressor in a species-specific manner, suggesting the plant cell walls may play an important role in determination of plant-fungal pathogen specificity.

AB - Fractions solubilized with NaCl from cell walls of pea and cowpea plants catalyzed the formation of blue formazan from nitroblue tetrazolium. Because superoxide dismutase decreased formazan production by over 90%, superoxide onion (O2-) may participate in the formation of formazan in the solubilized cell wall fractions. The formazan formation in the fractions solubilized from pea and cowpea cell walls was markedly reduced by exclusion of NAD(P)H, manganese ion, or p-coumaric acid from the reaction mixture. The formazan formation was severely inhibited by salicylhydroxamic acid and catalase, but not by imidazole, pyridine, quinacrine, and diphenyleneiodonium. An elicitor preparation from the pea pathogen Mycosphaerella pinodes enhanced the activities of formazan formation nonspecifically in both pea and cowpea fractions. The suppressor preparation from M. pinodes inhibited the activity in the pea fraction in the presence or absence of the elicitor. In the cowpea fraction, however, the suppressor did not inhibit the elicitor-enhanced activity, and the suppressor alone stimulated formazan formation. These results indicated that O2- generation in the fractions solubilized from pea and cowpea cell walls seems to be catalyzed by cell wall-bound peroxidase(s) and that the plant cell walls alone are able to respond to the elicitor nonspecifically and to the suppressor in a species-specific manner, suggesting the plant cell walls may play an important role in determination of plant-fungal pathogen specificity.

KW - Pisum sativum

KW - Species specificity

KW - Vigna sinensis

UR - http://www.scopus.com/inward/record.url?scp=0030824754&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030824754&partnerID=8YFLogxK

M3 - Article

C2 - 18945053

AN - SCOPUS:0030824754

VL - 87

SP - 846

EP - 852

JO - Phytopathology

JF - Phytopathology

SN - 0031-949X

IS - 8

ER -