99mTc-Annexin-V uptake in a rat model of variable ischemic severity and reperfusion time

Junichi Taki, Takahiro Higuchi, Atsuhiro Kawashima, Jonathan F. Tait, Akira Muramori, Ichiro Matsunari, Kenichi Nakajima, Jean Luc Vanderheyden, H. William Strauss

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: To determine whether mild to moderate ischemia that is not severe enough to induce myocardial infarction will cause myocardial cell damage or apoptosis, the 99mTc-Annexin-V (Tc-A) uptake was studied in groups of rats with various intervals of coronary occlusion and reperfusion times. Methods and Results: After left coronary artery occlusion for 15 min (n=23), 10 min (n=23), or 5min (n=12), Tc-A (80-150 MBq) was injected at 0.5, 1.5, 6, or 24 h after reperfusion. One hour later, to verify the area at risk, 201Tl (0.74 MBq) was injected just after left coronary artery re-occlusion and the rats were killed 1 min later. Dual tracer autoradiography was performed to assess Tc-A uptake and area at risk. In all 5-min occlusion and reperfusion models, no significant Tc-A uptake was observed in the area at risk. Tc-A uptake ratios in the 15-min and 10-min ischemia models were 4.46±3.16 and 2.02±0.47 (p=0.078) at 0.5 h after reperfusion, 3.49±1.78 and 1.47±0.11 (p<0.05) at 1.5h after reperfusion, 1.60±0.43 and 1.34±0.23 (p=0.24) at 6h after reperfusion, 1.50±0.33 and 1.28±0.33 (p=0.099) at 24 h after reperfusion, respectively. With 15-min ischemia, in 3 of the 5 rats there were a few micro-foci of myocardial cell degeneration and cell infiltration in less than 1% of the ischemic area at 24 h after reperfusion. No significant histological change was observed in rats with 10-min or 5-min ischemia. Conclusion: The data indicate that Tc-A binding depends on the severity of ischemia even without a significant amount of histological change or infarction.

Original languageEnglish
Pages (from-to)1141-1146
Number of pages6
JournalCirculation Journal
Volume71
Issue number7
DOIs
Publication statusPublished - Jul 4 2007
Externally publishedYes

Fingerprint

Annexin A5
Reperfusion
Ischemia
Coronary Occlusion
Coronary Vessels
Myocardial Reperfusion
Autoradiography
Infarction
Myocardial Infarction
Apoptosis

Keywords

  • Tc-Annexin- V
  • Apoptosis imaging
  • Myocardial ischemia
  • Reperfusion

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Taki, J., Higuchi, T., Kawashima, A., Tait, J. F., Muramori, A., Matsunari, I., ... Strauss, H. W. (2007). 99mTc-Annexin-V uptake in a rat model of variable ischemic severity and reperfusion time. Circulation Journal, 71(7), 1141-1146. https://doi.org/10.1253/circj.71.1141

99mTc-Annexin-V uptake in a rat model of variable ischemic severity and reperfusion time. / Taki, Junichi; Higuchi, Takahiro; Kawashima, Atsuhiro; Tait, Jonathan F.; Muramori, Akira; Matsunari, Ichiro; Nakajima, Kenichi; Vanderheyden, Jean Luc; Strauss, H. William.

In: Circulation Journal, Vol. 71, No. 7, 04.07.2007, p. 1141-1146.

Research output: Contribution to journalArticle

Taki, J, Higuchi, T, Kawashima, A, Tait, JF, Muramori, A, Matsunari, I, Nakajima, K, Vanderheyden, JL & Strauss, HW 2007, '99mTc-Annexin-V uptake in a rat model of variable ischemic severity and reperfusion time', Circulation Journal, vol. 71, no. 7, pp. 1141-1146. https://doi.org/10.1253/circj.71.1141
Taki, Junichi ; Higuchi, Takahiro ; Kawashima, Atsuhiro ; Tait, Jonathan F. ; Muramori, Akira ; Matsunari, Ichiro ; Nakajima, Kenichi ; Vanderheyden, Jean Luc ; Strauss, H. William. / 99mTc-Annexin-V uptake in a rat model of variable ischemic severity and reperfusion time. In: Circulation Journal. 2007 ; Vol. 71, No. 7. pp. 1141-1146.
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AU - Tait, Jonathan F.

AU - Muramori, Akira

AU - Matsunari, Ichiro

AU - Nakajima, Kenichi

AU - Vanderheyden, Jean Luc

AU - Strauss, H. William

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N2 - Background: To determine whether mild to moderate ischemia that is not severe enough to induce myocardial infarction will cause myocardial cell damage or apoptosis, the 99mTc-Annexin-V (Tc-A) uptake was studied in groups of rats with various intervals of coronary occlusion and reperfusion times. Methods and Results: After left coronary artery occlusion for 15 min (n=23), 10 min (n=23), or 5min (n=12), Tc-A (80-150 MBq) was injected at 0.5, 1.5, 6, or 24 h after reperfusion. One hour later, to verify the area at risk, 201Tl (0.74 MBq) was injected just after left coronary artery re-occlusion and the rats were killed 1 min later. Dual tracer autoradiography was performed to assess Tc-A uptake and area at risk. In all 5-min occlusion and reperfusion models, no significant Tc-A uptake was observed in the area at risk. Tc-A uptake ratios in the 15-min and 10-min ischemia models were 4.46±3.16 and 2.02±0.47 (p=0.078) at 0.5 h after reperfusion, 3.49±1.78 and 1.47±0.11 (p<0.05) at 1.5h after reperfusion, 1.60±0.43 and 1.34±0.23 (p=0.24) at 6h after reperfusion, 1.50±0.33 and 1.28±0.33 (p=0.099) at 24 h after reperfusion, respectively. With 15-min ischemia, in 3 of the 5 rats there were a few micro-foci of myocardial cell degeneration and cell infiltration in less than 1% of the ischemic area at 24 h after reperfusion. No significant histological change was observed in rats with 10-min or 5-min ischemia. Conclusion: The data indicate that Tc-A binding depends on the severity of ischemia even without a significant amount of histological change or infarction.

AB - Background: To determine whether mild to moderate ischemia that is not severe enough to induce myocardial infarction will cause myocardial cell damage or apoptosis, the 99mTc-Annexin-V (Tc-A) uptake was studied in groups of rats with various intervals of coronary occlusion and reperfusion times. Methods and Results: After left coronary artery occlusion for 15 min (n=23), 10 min (n=23), or 5min (n=12), Tc-A (80-150 MBq) was injected at 0.5, 1.5, 6, or 24 h after reperfusion. One hour later, to verify the area at risk, 201Tl (0.74 MBq) was injected just after left coronary artery re-occlusion and the rats were killed 1 min later. Dual tracer autoradiography was performed to assess Tc-A uptake and area at risk. In all 5-min occlusion and reperfusion models, no significant Tc-A uptake was observed in the area at risk. Tc-A uptake ratios in the 15-min and 10-min ischemia models were 4.46±3.16 and 2.02±0.47 (p=0.078) at 0.5 h after reperfusion, 3.49±1.78 and 1.47±0.11 (p<0.05) at 1.5h after reperfusion, 1.60±0.43 and 1.34±0.23 (p=0.24) at 6h after reperfusion, 1.50±0.33 and 1.28±0.33 (p=0.099) at 24 h after reperfusion, respectively. With 15-min ischemia, in 3 of the 5 rats there were a few micro-foci of myocardial cell degeneration and cell infiltration in less than 1% of the ischemic area at 24 h after reperfusion. No significant histological change was observed in rats with 10-min or 5-min ischemia. Conclusion: The data indicate that Tc-A binding depends on the severity of ischemia even without a significant amount of histological change or infarction.

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KW - Apoptosis imaging

KW - Myocardial ischemia

KW - Reperfusion

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