Sunflower centromeres consist of a centromere-specific LINE and a chromosome-specific tandem repeat

Kiyotaka Nagaki, Keisuke Tanaka, Naoki Yamaji, Hisato Kobayashi, Minoru Murata

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The kinetochore is a protein complex including kinetochore-specific proteins that plays a role in chromatid segregation during mitosis and meiosis. The complex associates with centromeric DNA sequences that are usually species-specific. In plant species, tandem repeats including satellite DNA sequences and retrotransposons have been reported as centromeric DNA sequences. In this study on sunflowers, a cDNA-encoding centromere-specific histone H3 (CENH3) was isolated from a cDNA pool from a seedling, and an antibody was raised against a peptide synthesized from the deduced cDNA. The antibody specifically recognized the sunflower CENH3 (HaCENH3) and showed centromeric signals by immunostaining and immunohistochemical staining analysis. The antibody was also applied in chromatin immunoprecipitation (ChIP)-Seq to isolate centromeric DNA sequences and two different types of repetitive DNA sequences were identified. One was a long interspersed nuclear element (LINE)-like sequence, which showed centromere-specific signals on almost all chromosomes in sunflowers. This is the first report of a centromeric LINE sequence, suggesting possible centromere targeting ability. Another type of identified repetitive DNA was a tandem repeat sequence with a 187-bp unit that was found only on a pair of chromosomes. The HaCENH3 content of the tandem repeats was estimated to be much higher than that of the LINE, which implies centromere evolution from LINE-based centromeres to more stable tandem-repeat-based centromeres. In addition, the epigenetic status of the sunflower centromeres was investigated by immunohistochemical staining and ChIP, and it was found that centromeres were heterochromatic.

Original languageEnglish
Article number912
JournalFrontiers in Plant Science
Volume6
Issue numberOCTOBER
DOIs
Publication statusPublished - Oct 31 2015

Fingerprint

tandem repeat sequences
centromeres
Helianthus annuus
chromosomes
nucleotide sequences
kinetochores
histones
antibodies
chromatin
satellite DNA
chromatids
retrotransposons
epigenetics
meiosis
mitosis
proteins
peptides
seedlings
DNA

Keywords

  • Centromere
  • Centromeric DNA
  • Centromeric histone H3
  • ChIP-seq
  • Sunflower (Helianthus annuus)

ASJC Scopus subject areas

  • Plant Science

Cite this

Sunflower centromeres consist of a centromere-specific LINE and a chromosome-specific tandem repeat. / Nagaki, Kiyotaka; Tanaka, Keisuke; Yamaji, Naoki; Kobayashi, Hisato; Murata, Minoru.

In: Frontiers in Plant Science, Vol. 6, No. OCTOBER, 912, 31.10.2015.

Research output: Contribution to journalArticle

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