Summer heat stress affects prostaglandin synthesis in the bovine oviduct

Yoshihiko Kobayashi, Kaori Wakamiya, Misa Kohka, Yuki Yamamoto, Kiyoshi Okuda

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Summer heat stress (HS) negatively affects reproductive functions, including prostaglandin (PG) F secretion in the endometrium, and decreases fertility in cattle. In the present study, we examined the effects of elevated temperatures on PG synthesis in oviductal epithelial cells. The epithelial cells obtained from the ampulla and isthmus of the oviduct were incubated at various temperatures (38.5, 39.5, 40.0, and 40.5 °C) for 24 h. In the ampulla, PGE2 concentration was higher at 40.5 °C than at 38.5 °C, while PGF production was not affected by the temperatures in this range. The expressions of microsomal PGE synthase 1 (PTGES (mPGES1)), cytosolic PGES (PTGES3 (cPGES)), and heat shock protein 90 (HSP90AA1 (HSP90)) mRNAs and proteins were higher at 40.5 °C than at 38.5 °C in the ampullary epithelial cells. Seasonal changes in the expressions of PGES and HSP90AA1 mRNAs in oviductal tissues were also investigated. The expressions of PTGES3 and HSP90AA1 mRNAs were higher in the ampullary tissues in summer than in winter. In summary, elevated temperatures stimulated PGE2 production in the ampullary oviduct by increasing the expressions of PGESs and HSP90AA1, which can activate cPGES. The overall results suggest that HS upsets PG secretions and reduces oviductal smooth muscle motility, which in turn could decrease gamete/embryo transport through the oviduct.

Original languageEnglish
Pages (from-to)103-110
Number of pages8
JournalReproduction
Volume146
Issue number2
DOIs
Publication statusPublished - Aug 2013

Fingerprint

Oviducts
Prostaglandins
Hot Temperature
Temperature
Dinoprost
Epithelial Cells
Dinoprostone
Messenger RNA
HSP90 Heat-Shock Proteins
Endometrium
Prostaglandins E
Germ Cells
Fertility
Smooth Muscle
Embryonic Structures
Proteins

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Reproductive Medicine
  • Embryology
  • Endocrinology
  • Cell Biology

Cite this

Summer heat stress affects prostaglandin synthesis in the bovine oviduct. / Kobayashi, Yoshihiko; Wakamiya, Kaori; Kohka, Misa; Yamamoto, Yuki; Okuda, Kiyoshi.

In: Reproduction, Vol. 146, No. 2, 08.2013, p. 103-110.

Research output: Contribution to journalArticle

Kobayashi, Y, Wakamiya, K, Kohka, M, Yamamoto, Y & Okuda, K 2013, 'Summer heat stress affects prostaglandin synthesis in the bovine oviduct', Reproduction, vol. 146, no. 2, pp. 103-110. https://doi.org/10.1530/REP-12-0479
Kobayashi, Yoshihiko ; Wakamiya, Kaori ; Kohka, Misa ; Yamamoto, Yuki ; Okuda, Kiyoshi. / Summer heat stress affects prostaglandin synthesis in the bovine oviduct. In: Reproduction. 2013 ; Vol. 146, No. 2. pp. 103-110.
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