Sugar-responsive gene expression and the agr system are required for colony spreading in Staphylococcus aureus

Tomofumi Ueda, Chikara Kaito, Yosuke Omae, Kazuhisa Sekimizu

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Staphylococcus aureus spreads on soft agar surfaces, which is called "colony spreading" Here, we report that the colony spreading in S. aureus was promoted by the addition of glucose to soft agar plates. Disruption of ccpA and hprK, which are involved in catabolite repression, decreased the colony spreading ability promoted by glucose. Deletion of the agr locus, a virulence regulatory element whose expression is activated by glucose in a ccpA-dependent manner, abolished the colony spreading promoted by glucose. Disruption of clpP and arlRS, which contributes to agr expression, also decreased glucose-promoted colony spreading. These findings suggest that S. aureus colony spreading requires the expression of agr, which is positively regulated by environmental carbon sources, and that virulence gene expression and colony spreading induced by agr are simultaneously activated in the S. aureus infectious process.

Original languageEnglish
Pages (from-to)178-185
Number of pages8
JournalMicrobial Pathogenesis
Volume51
Issue number3
DOIs
Publication statusPublished - Sep 1 2011
Externally publishedYes

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Keywords

  • Agr
  • Catabolite repression
  • Colony spreading
  • Staphylococcus aureus
  • Virulence

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

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