Sugar-induced increase in cytosolic Ca2+ in Arabidopsis thaliana whole plants

Takuya Furuichi, Izumi Mori, Koji Takahashi, Shoshi Muto

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Using Ca2+-dependent photoprotein aequorin-transformed Arabidopsis thaliana, sugar-induced increase in cytosolic free Ca2+ concentration ([Ca2+]cyt) was investigated by luminescence imaging technique. When 0.1 M sucrose was fed to roots of autotrophically grown intact whole plants whose roots had been incubated overnight with coelenterazine to reconstitute aequorin systemically, strong and transient (within 20 s) luminescence was observed in the roots; that luminescence was followed by weak luminescence moving from the lower leaves to the upper leaves. The moving rate of luminescence was roughly comparable to that of [14C]sucrose. Application of 0.1 M glucose or fructose induced transient luminescence in excised leaves. No such luminescence was observed in heterotrophically grown (with sucrose) whole plants or in excised tissues. mRNA levels of sucrose-H+ symporter genes AtSUC1 and AtSUC2 were higher in autotrophic plants than in heterotrophic plants. These results indicate that influx of transported sucrose together with H+ into the mesophyll cells of autotrophic plants may depolarize the membrane potential, and subsequently activate a voltage-gated Ca2+ channel on the plasma membrane, resulting in a [Ca2+]cyt increase. The [Ca2+]cyt increase might initiate Ca2+ signaling leading to the expression of genes related to biosynthesis of storage carbohydrates. Hexoses, when applied, might also be involved in the [Ca2+]cyt increase mediated by monosaccharide-H+ co-transporters.

Original languageEnglish
Pages (from-to)1149-1155
Number of pages7
JournalPlant and Cell Physiology
Volume42
Issue number10
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Luminescence
luminescence
Arabidopsis
Arabidopsis thaliana
sugars
calcium
Sucrose
sucrose
Aequorin
Symporters
symporters
Luminescent Proteins
Mesophyll Cells
Plant Roots
Hexoses
Monosaccharides
leaves
Fructose
Membrane Potentials
hexoses

Keywords

  • Aequorin
  • Arabidopsis thaliana
  • Calcium signaling
  • Luminescence imaging
  • Sucrose-H symporter
  • Sugar signal transduction

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Sugar-induced increase in cytosolic Ca2+ in Arabidopsis thaliana whole plants. / Furuichi, Takuya; Mori, Izumi; Takahashi, Koji; Muto, Shoshi.

In: Plant and Cell Physiology, Vol. 42, No. 10, 2001, p. 1149-1155.

Research output: Contribution to journalArticle

Furuichi, T, Mori, I, Takahashi, K & Muto, S 2001, 'Sugar-induced increase in cytosolic Ca2+ in Arabidopsis thaliana whole plants', Plant and Cell Physiology, vol. 42, no. 10, pp. 1149-1155.
Furuichi, Takuya ; Mori, Izumi ; Takahashi, Koji ; Muto, Shoshi. / Sugar-induced increase in cytosolic Ca2+ in Arabidopsis thaliana whole plants. In: Plant and Cell Physiology. 2001 ; Vol. 42, No. 10. pp. 1149-1155.
@article{f7313b4d60a7457c85cf7711f95921fc,
title = "Sugar-induced increase in cytosolic Ca2+ in Arabidopsis thaliana whole plants",
abstract = "Using Ca2+-dependent photoprotein aequorin-transformed Arabidopsis thaliana, sugar-induced increase in cytosolic free Ca2+ concentration ([Ca2+]cyt) was investigated by luminescence imaging technique. When 0.1 M sucrose was fed to roots of autotrophically grown intact whole plants whose roots had been incubated overnight with coelenterazine to reconstitute aequorin systemically, strong and transient (within 20 s) luminescence was observed in the roots; that luminescence was followed by weak luminescence moving from the lower leaves to the upper leaves. The moving rate of luminescence was roughly comparable to that of [14C]sucrose. Application of 0.1 M glucose or fructose induced transient luminescence in excised leaves. No such luminescence was observed in heterotrophically grown (with sucrose) whole plants or in excised tissues. mRNA levels of sucrose-H+ symporter genes AtSUC1 and AtSUC2 were higher in autotrophic plants than in heterotrophic plants. These results indicate that influx of transported sucrose together with H+ into the mesophyll cells of autotrophic plants may depolarize the membrane potential, and subsequently activate a voltage-gated Ca2+ channel on the plasma membrane, resulting in a [Ca2+]cyt increase. The [Ca2+]cyt increase might initiate Ca2+ signaling leading to the expression of genes related to biosynthesis of storage carbohydrates. Hexoses, when applied, might also be involved in the [Ca2+]cyt increase mediated by monosaccharide-H+ co-transporters.",
keywords = "Aequorin, Arabidopsis thaliana, Calcium signaling, Luminescence imaging, Sucrose-H symporter, Sugar signal transduction",
author = "Takuya Furuichi and Izumi Mori and Koji Takahashi and Shoshi Muto",
year = "2001",
language = "English",
volume = "42",
pages = "1149--1155",
journal = "Plant and Cell Physiology",
issn = "0032-0781",
publisher = "Oxford University Press",
number = "10",

}

TY - JOUR

T1 - Sugar-induced increase in cytosolic Ca2+ in Arabidopsis thaliana whole plants

AU - Furuichi, Takuya

AU - Mori, Izumi

AU - Takahashi, Koji

AU - Muto, Shoshi

PY - 2001

Y1 - 2001

N2 - Using Ca2+-dependent photoprotein aequorin-transformed Arabidopsis thaliana, sugar-induced increase in cytosolic free Ca2+ concentration ([Ca2+]cyt) was investigated by luminescence imaging technique. When 0.1 M sucrose was fed to roots of autotrophically grown intact whole plants whose roots had been incubated overnight with coelenterazine to reconstitute aequorin systemically, strong and transient (within 20 s) luminescence was observed in the roots; that luminescence was followed by weak luminescence moving from the lower leaves to the upper leaves. The moving rate of luminescence was roughly comparable to that of [14C]sucrose. Application of 0.1 M glucose or fructose induced transient luminescence in excised leaves. No such luminescence was observed in heterotrophically grown (with sucrose) whole plants or in excised tissues. mRNA levels of sucrose-H+ symporter genes AtSUC1 and AtSUC2 were higher in autotrophic plants than in heterotrophic plants. These results indicate that influx of transported sucrose together with H+ into the mesophyll cells of autotrophic plants may depolarize the membrane potential, and subsequently activate a voltage-gated Ca2+ channel on the plasma membrane, resulting in a [Ca2+]cyt increase. The [Ca2+]cyt increase might initiate Ca2+ signaling leading to the expression of genes related to biosynthesis of storage carbohydrates. Hexoses, when applied, might also be involved in the [Ca2+]cyt increase mediated by monosaccharide-H+ co-transporters.

AB - Using Ca2+-dependent photoprotein aequorin-transformed Arabidopsis thaliana, sugar-induced increase in cytosolic free Ca2+ concentration ([Ca2+]cyt) was investigated by luminescence imaging technique. When 0.1 M sucrose was fed to roots of autotrophically grown intact whole plants whose roots had been incubated overnight with coelenterazine to reconstitute aequorin systemically, strong and transient (within 20 s) luminescence was observed in the roots; that luminescence was followed by weak luminescence moving from the lower leaves to the upper leaves. The moving rate of luminescence was roughly comparable to that of [14C]sucrose. Application of 0.1 M glucose or fructose induced transient luminescence in excised leaves. No such luminescence was observed in heterotrophically grown (with sucrose) whole plants or in excised tissues. mRNA levels of sucrose-H+ symporter genes AtSUC1 and AtSUC2 were higher in autotrophic plants than in heterotrophic plants. These results indicate that influx of transported sucrose together with H+ into the mesophyll cells of autotrophic plants may depolarize the membrane potential, and subsequently activate a voltage-gated Ca2+ channel on the plasma membrane, resulting in a [Ca2+]cyt increase. The [Ca2+]cyt increase might initiate Ca2+ signaling leading to the expression of genes related to biosynthesis of storage carbohydrates. Hexoses, when applied, might also be involved in the [Ca2+]cyt increase mediated by monosaccharide-H+ co-transporters.

KW - Aequorin

KW - Arabidopsis thaliana

KW - Calcium signaling

KW - Luminescence imaging

KW - Sucrose-H symporter

KW - Sugar signal transduction

UR - http://www.scopus.com/inward/record.url?scp=0034761765&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034761765&partnerID=8YFLogxK

M3 - Article

C2 - 11673631

AN - SCOPUS:0034761765

VL - 42

SP - 1149

EP - 1155

JO - Plant and Cell Physiology

JF - Plant and Cell Physiology

SN - 0032-0781

IS - 10

ER -