TY - JOUR
T1 - Sucrose induces fatty liver and pancreatic inflammation in male breeder rats independent of excess energy intake
AU - Roncal-Jimenez, Carlos A.
AU - Lanaspa, Miguel A.
AU - Rivard, Christopher J.
AU - Nakagawa, Takahiko
AU - Sanchez-Lozada, L. Gabriela
AU - Jalal, Diana
AU - Andres-Hernando, Ana
AU - Tanabe, Katsuyuki
AU - Madero, Magdalena
AU - Li, Nanxing
AU - Cicerchi, Christina
AU - McFann, Kim
AU - Sautin, Yuri Y.
AU - Johnson, Richard J.
N1 - Funding Information:
Supported by National Institutes of Health grant HL-68607 and startup funds at the University of Colorado (RJJ) .
PY - 2011/9
Y1 - 2011/9
N2 - Fructose induces metabolic syndrome in rats; but studies have been criticized for using high concentrations of fructose that are not physiologic, for using only pure fructose, and for not controlling for energy intake. We tested the hypothesis that a 40% sucrose diet (containing 20% fructose) might induce features of metabolic syndrome in male breeder rats independent of excess energy intake. Male Sprague-Dawley breeder rats were pair fed 40% sucrose or isocaloric starch diet for 4 months and evaluated for metabolic syndrome and diabetes. In vitro studies were performed in rat insulinoma cells (RIN-m5F) exposed to uric acid, and markers of inflammation were assessed. Rats fed a 40% sucrose diet developed accelerated features of metabolic syndrome with up-regulation of fructose-dependent transporter Glut5 and fructokinase. Fatty liver and low-grade pancreatic inflammation also occurred. Uric acid was found to stimulate inflammatory mediators and oxidative stress in islet cells in vitro. Sucrose, at concentrations ingested by a subset of Americans, can accelerate metabolic syndrome, fatty liver, and type 2 diabetes mellitus in male breeder rats; and the effects are independent of excess energy intake.
AB - Fructose induces metabolic syndrome in rats; but studies have been criticized for using high concentrations of fructose that are not physiologic, for using only pure fructose, and for not controlling for energy intake. We tested the hypothesis that a 40% sucrose diet (containing 20% fructose) might induce features of metabolic syndrome in male breeder rats independent of excess energy intake. Male Sprague-Dawley breeder rats were pair fed 40% sucrose or isocaloric starch diet for 4 months and evaluated for metabolic syndrome and diabetes. In vitro studies were performed in rat insulinoma cells (RIN-m5F) exposed to uric acid, and markers of inflammation were assessed. Rats fed a 40% sucrose diet developed accelerated features of metabolic syndrome with up-regulation of fructose-dependent transporter Glut5 and fructokinase. Fatty liver and low-grade pancreatic inflammation also occurred. Uric acid was found to stimulate inflammatory mediators and oxidative stress in islet cells in vitro. Sucrose, at concentrations ingested by a subset of Americans, can accelerate metabolic syndrome, fatty liver, and type 2 diabetes mellitus in male breeder rats; and the effects are independent of excess energy intake.
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U2 - 10.1016/j.metabol.2011.01.008
DO - 10.1016/j.metabol.2011.01.008
M3 - Article
C2 - 21489572
AN - SCOPUS:80051784027
VL - 60
SP - 1259
EP - 1270
JO - Metabolism: Clinical and Experimental
JF - Metabolism: Clinical and Experimental
SN - 0026-0495
IS - 9
ER -
