Successful steroid pulse therapy for brain lesion caused by Shiga toxin 2 in rabbits

Jun Fujii, Yoshimasa Kinoshita, Akihiro Matsukawa, Sharon Y A M Villanueva, Takashi Yutsudo, Shin ichi Yoshida

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Betamethasone sodium phosphate (BSP) is usually used as a steroid therapy for human brain edema. High doses of BSP (36 mg/kg) twice a day for two days statistically reduced the mortality rate and improved the survival period of Stx2 (1.4 μg/kg; 1.6LD50)-toxemic rabbits. We made evaluations on three kinds of magnetic resonance images (MRI) including T1-weighted, T2-weighted, and enhanced MRI using gadopentetate dimeglumine (Gd-DTPA) to detect brain lesion induced by an intravenous injection of Stx2 in rabbits. Enhanced T1-weighted MRI was the most sensitive tool to find leakage of Gd-DTPA suggesting impairment of the blood brain barrier caused by Stx2. Enhanced MRI revealed that BSP treatment inhibited the leakage of Gd-DTPA, as directly evidenced by the protective effect of BSP against brain edema induced by intravenous injection of Stx2. Interleukin 1β was not induced after Stx2 treatment in brain primary mixed cell culture. Crown

Original languageEnglish
Pages (from-to)179-184
Number of pages6
JournalMicrobial Pathogenesis
Volume46
Issue number4
DOIs
Publication statusPublished - Apr 2009

    Fingerprint

Keywords

  • Brain
  • MRI
  • O157
  • Pulse therapy
  • Steroid
  • Stx2

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Cite this