Abstract
Current clinical reports have indicated that hepatocyte transplantation (HTX) could be used in patients with liver failure and in children with liver-based metabolic diseases. One of the major limiting factors of HTX is a serious shortage of donor livers for hepatocyte isolation. To address this issue, we immortalized adult human hepatocytes with a retroviral vector SSR# 69 expressing the genes of simian virus 40 large T antigen and herpes simplex virus-thymidine kinase simultaneously. One of the resulting clones, NKNT-3, grew steadily in chemically defined serum-free medium without any obvious crisis and showed the gene expression of differentiated liver functions. Under the administration of 5 μM ganciclovir, NKNT-3 cells stopped proliferation and died in in vitro experiments. We have established a tightly regulated immortal human hepatocyte cell line. The cells could allow the need for immediate availability of consistent and functionally uniform cells in sufficient quantity and adequate quality.
| Original language | English |
|---|---|
| Pages (from-to) | 377-381 |
| Number of pages | 5 |
| Journal | Cell Transplantation |
| Volume | 10 |
| Issue number | 4-5 |
| Publication status | Published - 2001 |
Fingerprint
Keywords
- Immortalized human hepatocyte
- Retroviral gene transfer
- Simian virus 40 large T antigen
ASJC Scopus subject areas
- Cell Biology
- Transplantation
Cite this
Successful retroviral gene transfer of simian virus 40 T antigen and herpes simplex virus-thymidine kinase into human hepatocytes. / Kobayashi, N.; Noguchi, H.; Westerman, K. A.; Watanabe, T.; Matsumura, T.; Totsugawa, T.; Fujiwara, Toshiyoshi; Leboulch, P.; Tanaka, N.
In: Cell Transplantation, Vol. 10, No. 4-5, 2001, p. 377-381.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Successful retroviral gene transfer of simian virus 40 T antigen and herpes simplex virus-thymidine kinase into human hepatocytes
AU - Kobayashi, N.
AU - Noguchi, H.
AU - Westerman, K. A.
AU - Watanabe, T.
AU - Matsumura, T.
AU - Totsugawa, T.
AU - Fujiwara, Toshiyoshi
AU - Leboulch, P.
AU - Tanaka, N.
PY - 2001
Y1 - 2001
N2 - Current clinical reports have indicated that hepatocyte transplantation (HTX) could be used in patients with liver failure and in children with liver-based metabolic diseases. One of the major limiting factors of HTX is a serious shortage of donor livers for hepatocyte isolation. To address this issue, we immortalized adult human hepatocytes with a retroviral vector SSR# 69 expressing the genes of simian virus 40 large T antigen and herpes simplex virus-thymidine kinase simultaneously. One of the resulting clones, NKNT-3, grew steadily in chemically defined serum-free medium without any obvious crisis and showed the gene expression of differentiated liver functions. Under the administration of 5 μM ganciclovir, NKNT-3 cells stopped proliferation and died in in vitro experiments. We have established a tightly regulated immortal human hepatocyte cell line. The cells could allow the need for immediate availability of consistent and functionally uniform cells in sufficient quantity and adequate quality.
AB - Current clinical reports have indicated that hepatocyte transplantation (HTX) could be used in patients with liver failure and in children with liver-based metabolic diseases. One of the major limiting factors of HTX is a serious shortage of donor livers for hepatocyte isolation. To address this issue, we immortalized adult human hepatocytes with a retroviral vector SSR# 69 expressing the genes of simian virus 40 large T antigen and herpes simplex virus-thymidine kinase simultaneously. One of the resulting clones, NKNT-3, grew steadily in chemically defined serum-free medium without any obvious crisis and showed the gene expression of differentiated liver functions. Under the administration of 5 μM ganciclovir, NKNT-3 cells stopped proliferation and died in in vitro experiments. We have established a tightly regulated immortal human hepatocyte cell line. The cells could allow the need for immediate availability of consistent and functionally uniform cells in sufficient quantity and adequate quality.
KW - Immortalized human hepatocyte
KW - Retroviral gene transfer
KW - Simian virus 40 large T antigen
UR - http://www.scopus.com/inward/record.url?scp=0034873040&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034873040&partnerID=8YFLogxK
M3 - Article
C2 - 11549057
AN - SCOPUS:0034873040
VL - 10
SP - 377
EP - 381
JO - Cell Transplantation
JF - Cell Transplantation
SN - 0963-6897
IS - 4-5
ER -