Successful retroviral gene transfer of simian virus 40 T antigen and herpes simplex virus-thymidine kinase into human hepatocytes

N. Kobayashi, H. Noguchi, K. A. Westerman, T. Watanabe, T. Matsumura, T. Totsugawa, T. Fujiwara, P. Leboulch, N. Tanaka

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Current clinical reports have indicated that hepatocyte transplantation (HTX) could be used in patients with liver failure and in children with liver-based metabolic diseases. One of the major limiting factors of HTX is a serious shortage of donor livers for hepatocyte isolation. To address this issue, we immortalized adult human hepatocytes with a retroviral vector SSR# 69 expressing the genes of simian virus 40 large T antigen and herpes simplex virus-thymidine kinase simultaneously. One of the resulting clones, NKNT-3, grew steadily in chemically defined serum-free medium without any obvious crisis and showed the gene expression of differentiated liver functions. Under the administration of 5 μM ganciclovir, NKNT-3 cells stopped proliferation and died in in vitro experiments. We have established a tightly regulated immortal human hepatocyte cell line. The cells could allow the need for immediate availability of consistent and functionally uniform cells in sufficient quantity and adequate quality.

Original languageEnglish
Pages (from-to)377-381
Number of pages5
JournalCell Transplantation
Volume10
Issue number4-5
DOIs
Publication statusPublished - 2001

Keywords

  • Immortalized human hepatocyte
  • Retroviral gene transfer
  • Simian virus 40 large T antigen

ASJC Scopus subject areas

  • Biomedical Engineering
  • Cell Biology
  • Transplantation

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    Kobayashi, N., Noguchi, H., Westerman, K. A., Watanabe, T., Matsumura, T., Totsugawa, T., Fujiwara, T., Leboulch, P., & Tanaka, N. (2001). Successful retroviral gene transfer of simian virus 40 T antigen and herpes simplex virus-thymidine kinase into human hepatocytes. Cell Transplantation, 10(4-5), 377-381. https://doi.org/10.3727/000000001783986585