Successful retroviral gene transfer of simian virus 40 T antigen and herpes simplex virus-thymidine kinase into human hepatocytes

N. Kobayashi, H. Noguchi, K. A. Westerman, T. Watanabe, T. Matsumura, T. Totsugawa, Toshiyoshi Fujiwara, P. Leboulch, N. Tanaka

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Current clinical reports have indicated that hepatocyte transplantation (HTX) could be used in patients with liver failure and in children with liver-based metabolic diseases. One of the major limiting factors of HTX is a serious shortage of donor livers for hepatocyte isolation. To address this issue, we immortalized adult human hepatocytes with a retroviral vector SSR# 69 expressing the genes of simian virus 40 large T antigen and herpes simplex virus-thymidine kinase simultaneously. One of the resulting clones, NKNT-3, grew steadily in chemically defined serum-free medium without any obvious crisis and showed the gene expression of differentiated liver functions. Under the administration of 5 μM ganciclovir, NKNT-3 cells stopped proliferation and died in in vitro experiments. We have established a tightly regulated immortal human hepatocyte cell line. The cells could allow the need for immediate availability of consistent and functionally uniform cells in sufficient quantity and adequate quality.

Original languageEnglish
Pages (from-to)377-381
Number of pages5
JournalCell Transplantation
Volume10
Issue number4-5
Publication statusPublished - 2001

Fingerprint

Gene transfer
Simian virus 40
Thymidine Kinase
Viral Tumor Antigens
Simplexvirus
Antigens
Viruses
Liver
Hepatocytes
Genes
Ganciclovir
Metabolic Diseases
Serum-Free Culture Media
Liver Failure
Cell proliferation
Gene expression
Clone Cells
Transplantation
Cells
Cell Proliferation

Keywords

  • Immortalized human hepatocyte
  • Retroviral gene transfer
  • Simian virus 40 large T antigen

ASJC Scopus subject areas

  • Cell Biology
  • Transplantation

Cite this

Kobayashi, N., Noguchi, H., Westerman, K. A., Watanabe, T., Matsumura, T., Totsugawa, T., ... Tanaka, N. (2001). Successful retroviral gene transfer of simian virus 40 T antigen and herpes simplex virus-thymidine kinase into human hepatocytes. Cell Transplantation, 10(4-5), 377-381.

Successful retroviral gene transfer of simian virus 40 T antigen and herpes simplex virus-thymidine kinase into human hepatocytes. / Kobayashi, N.; Noguchi, H.; Westerman, K. A.; Watanabe, T.; Matsumura, T.; Totsugawa, T.; Fujiwara, Toshiyoshi; Leboulch, P.; Tanaka, N.

In: Cell Transplantation, Vol. 10, No. 4-5, 2001, p. 377-381.

Research output: Contribution to journalArticle

Kobayashi, N, Noguchi, H, Westerman, KA, Watanabe, T, Matsumura, T, Totsugawa, T, Fujiwara, T, Leboulch, P & Tanaka, N 2001, 'Successful retroviral gene transfer of simian virus 40 T antigen and herpes simplex virus-thymidine kinase into human hepatocytes', Cell Transplantation, vol. 10, no. 4-5, pp. 377-381.
Kobayashi N, Noguchi H, Westerman KA, Watanabe T, Matsumura T, Totsugawa T et al. Successful retroviral gene transfer of simian virus 40 T antigen and herpes simplex virus-thymidine kinase into human hepatocytes. Cell Transplantation. 2001;10(4-5):377-381.
Kobayashi, N. ; Noguchi, H. ; Westerman, K. A. ; Watanabe, T. ; Matsumura, T. ; Totsugawa, T. ; Fujiwara, Toshiyoshi ; Leboulch, P. ; Tanaka, N. / Successful retroviral gene transfer of simian virus 40 T antigen and herpes simplex virus-thymidine kinase into human hepatocytes. In: Cell Transplantation. 2001 ; Vol. 10, No. 4-5. pp. 377-381.
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