Successful nonsurgical transfer of bovine elongating conceptuses and its application to sexing

Koji Kimura, Shuichi Matsuyama

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The objectives of the present study were to establish a nonsurgical transfer method for elongating bovine conceptuses and to combine this method with biopsy and sexing. Bovine conceptuses were recovered from donor cows on days 13-14 of the estrus cycle. In experiment 1, day 13 conceptuses were transferred to recipient cows using a standard day 7 embryo transfer (ET) method. The pregnancy rate of day 13 conceptus transfer (CT) is comparable to that of day 7 ET. In experiment 2, day 14 conceptuses were transferred using modified methods (balloon catheters or ET guns with modified sheaths). Using the standard ET method, no pregnancies were obtained; however, when balloon catheters or ET guns with modified sheaths were used, the pregnancy rates after CT were 48.0% and 44.8%, respectively. In experiment 3, day 14 conceptuses were biopsied without a micromanipulator, sexed using the loop-mediated isothermal amplification method and transferred to recipient cows. The pregnancy rate of biopsied conceptuses was 46.2% and did not differ significantly from that of unbiopsied conceptuses. Moreover, all pregnant cows transferred conceptuses following biopsy and sexing delivered calves with the expected sexes. These results suggested that the nonsurgical bovine CT method was comparable to day 7 ET and that this technique enables biopsy and sexing without expensive equipment such as a micromanipulator or specialized skills.

Original languageEnglish
Pages (from-to)210-215
Number of pages6
JournalJournal of Reproduction and Development
Volume60
Issue number3
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

conceptus
sex determination analysis
Embryo Transfer
embryo transfer
cattle
Pregnancy Rate
Firearms
Biopsy
pregnancy rate
cows
biopsy
Catheters
methodology
catheters
Estrus
Equipment and Supplies
Pregnancy
estrous cycle
pregnancy
calves

Keywords

  • Biopsy
  • Cattle
  • Conceptus
  • Sexing
  • Transfer

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Medicine(all)

Cite this

Successful nonsurgical transfer of bovine elongating conceptuses and its application to sexing. / Kimura, Koji; Matsuyama, Shuichi.

In: Journal of Reproduction and Development, Vol. 60, No. 3, 2014, p. 210-215.

Research output: Contribution to journalArticle

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