Subgenomic replicon derived from a cell line infected with the hepatitis C virus

Hiroe Kishine, Kazuo Sugiyama, Makoto Hijikata, Nobuyuki Kato, Hitoshi Takahashi, Takeshi Noshi, Yasunori Nio, Masahiro Hosaka, Yusuke Miyanari, Kunitada Shimotohno

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Recently, cell culture systems have been established, where a hepatitis C virus (HCV) subgenomic replicon was efficiently replicated and maintained for a long period. To see whether a HCV sequence derived from HCV-infected cultured cell sequence can be used for the construction of a functional replicon, a HCV subgenomic RNA carrying a neomycin-resistant gene was constructed using the HCV genome RNA obtained from cultured cells infected with HCV. After transfection, G418-resistant Huh-7 cells were selected and subcloned. Finally, the production of HCV proteins and de novo synthesis of subgenomic RNA were confirmed in the selected cell clone, indicating that this subgenomic RNA replicated in cultured cells and functioned as a replicon. These results suggest that the HCV genome obtained from an in vitro HCV infection system with cultured cells can be used to develop a sub-genomic replicon system with diverse HCV sequences.

Original languageEnglish
Pages (from-to)993-999
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume293
Issue number3
DOIs
Publication statusPublished - 2002

Fingerprint

Replicon
Viruses
Hepacivirus
Cells
Cell Line
Cultured Cells
RNA
Genes
Clone cells
Genome
Neomycin
Virus Diseases
Cell culture
Transfection
Clone Cells
Cell Culture Techniques

Keywords

  • Hepatitis C virus
  • Huh-7
  • Replication
  • Replicon

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Subgenomic replicon derived from a cell line infected with the hepatitis C virus. / Kishine, Hiroe; Sugiyama, Kazuo; Hijikata, Makoto; Kato, Nobuyuki; Takahashi, Hitoshi; Noshi, Takeshi; Nio, Yasunori; Hosaka, Masahiro; Miyanari, Yusuke; Shimotohno, Kunitada.

In: Biochemical and Biophysical Research Communications, Vol. 293, No. 3, 2002, p. 993-999.

Research output: Contribution to journalArticle

Kishine, H, Sugiyama, K, Hijikata, M, Kato, N, Takahashi, H, Noshi, T, Nio, Y, Hosaka, M, Miyanari, Y & Shimotohno, K 2002, 'Subgenomic replicon derived from a cell line infected with the hepatitis C virus', Biochemical and Biophysical Research Communications, vol. 293, no. 3, pp. 993-999. https://doi.org/10.1016/S0006-291X(02)00342-X
Kishine, Hiroe ; Sugiyama, Kazuo ; Hijikata, Makoto ; Kato, Nobuyuki ; Takahashi, Hitoshi ; Noshi, Takeshi ; Nio, Yasunori ; Hosaka, Masahiro ; Miyanari, Yusuke ; Shimotohno, Kunitada. / Subgenomic replicon derived from a cell line infected with the hepatitis C virus. In: Biochemical and Biophysical Research Communications. 2002 ; Vol. 293, No. 3. pp. 993-999.
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