Studying plant salt tolerance with the voltage clamp technique

Zhong Hua Chen, Dezhi Wu, Cornelia Eisenach, Adrian Hills, Guoping Zhang, Michael R. Blatt

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Voltage clamp is one of the key techniques for the dissection, identification, and monitoring of ion transporters in plant cells. Voltage clamp-based research work on salinity stress in plants enables the characterization of many plant ATP-dependent pumps, ion channels, and ion-coupled carriers through heterologous expression in Xenopus laevis oocytes and in vivo measurements in salt-tolerant and salt-sensitive giant green algae such as Chara and many plant species. We have modified and developed a reliable set of procedures for voltage clamp analysis in intact guard cells and root epidermal cells from Arabidopsis thaliana with potentially broad applications in the salinity response of plants. These procedures greatly extend the duration of measurements and scope for analysis of the predominant K+ and anion channels.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages19-33
Number of pages15
Volume913
ISBN (Print)9781617799853
DOIs
Publication statusPublished - 2012
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume913
ISSN (Print)10643745

Fingerprint

Salt-Tolerance
Patch-Clamp Techniques
Salinity
Salts
Chara
Ions
Chlorophyta
Xenopus laevis
Plant Cells
Ion Channels
Arabidopsis
Oocytes
Anions
Dissection
Adenosine Triphosphate
Research

Keywords

  • Arabidopsis thaliana
  • Guard cell
  • Microelectrode
  • Root epidermal cell
  • Voltage clamp

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Chen, Z. H., Wu, D., Eisenach, C., Hills, A., Zhang, G., & Blatt, M. R. (2012). Studying plant salt tolerance with the voltage clamp technique. In Methods in Molecular Biology (Vol. 913, pp. 19-33). (Methods in Molecular Biology; Vol. 913). Humana Press Inc.. https://doi.org/10.1007/978-1-61779-986-0_2

Studying plant salt tolerance with the voltage clamp technique. / Chen, Zhong Hua; Wu, Dezhi; Eisenach, Cornelia; Hills, Adrian; Zhang, Guoping; Blatt, Michael R.

Methods in Molecular Biology. Vol. 913 Humana Press Inc., 2012. p. 19-33 (Methods in Molecular Biology; Vol. 913).

Research output: Chapter in Book/Report/Conference proceedingChapter

Chen, ZH, Wu, D, Eisenach, C, Hills, A, Zhang, G & Blatt, MR 2012, Studying plant salt tolerance with the voltage clamp technique. in Methods in Molecular Biology. vol. 913, Methods in Molecular Biology, vol. 913, Humana Press Inc., pp. 19-33. https://doi.org/10.1007/978-1-61779-986-0_2
Chen ZH, Wu D, Eisenach C, Hills A, Zhang G, Blatt MR. Studying plant salt tolerance with the voltage clamp technique. In Methods in Molecular Biology. Vol. 913. Humana Press Inc. 2012. p. 19-33. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-61779-986-0_2
Chen, Zhong Hua ; Wu, Dezhi ; Eisenach, Cornelia ; Hills, Adrian ; Zhang, Guoping ; Blatt, Michael R. / Studying plant salt tolerance with the voltage clamp technique. Methods in Molecular Biology. Vol. 913 Humana Press Inc., 2012. pp. 19-33 (Methods in Molecular Biology).
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