Study on the effects of chloride depletion on photosystem II using different chloride depletion methods

Anjana Jajoo, Sonal Mathur, Pooja Mehta, Miho Nishimura, Suleyman Allakhverdiev, Yasusi Yamamoto

Research output: Contribution to journalArticle

Abstract

Chloride is an indispensable factor for the functioning of oxygen evolving complex (OEC) and has protective and activating effects on photosystem II. In this study we have investigated mainly by EPR, the properties of chloride-sufficient, chloride-deficient and chloride-depleted thylakoid membranes and photosystem II enriched membranes from spinach. The results on the effects of different chloride depletion methods on the structural and functional aspects of photosystem II showed that chloride-depletion by treating PS II membranes with high pH is a relatively harsh way causing a significant and irreparable damage to the PS II donor side. Damage to the acceptor side of PS II was recovered almost fully in chloride-deficient as well as chloride-depleted PS II membranes.

Original languageEnglish
Pages (from-to)47-53
Number of pages7
JournalJournal of Bioenergetics and Biomembranes
Volume42
Issue number1
DOIs
Publication statusPublished - Feb 2010

Fingerprint

Photosystem II Protein Complex
Chlorides
Membranes
Thylakoids
Spinacia oleracea
Oxygen

Keywords

  • Chloride
  • EPR
  • Oxygen evolving complex
  • Photosystem II

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

Cite this

Study on the effects of chloride depletion on photosystem II using different chloride depletion methods. / Jajoo, Anjana; Mathur, Sonal; Mehta, Pooja; Nishimura, Miho; Allakhverdiev, Suleyman; Yamamoto, Yasusi.

In: Journal of Bioenergetics and Biomembranes, Vol. 42, No. 1, 02.2010, p. 47-53.

Research output: Contribution to journalArticle

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