Study of the interaction between actin and antitumor substance aplyronine A with a novel fluorescent photoaffinity probe

Takeshi Kuroda, Kiyotake Suenaga, Akira Sakakura, Tomohisa Handa, Kazuhito Okamoto, Hideo Kigoshi

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The interaction between actin and aplyronine A, a potent antitumor and actin-depolymerizing substance of marine origin, was investigated by photoaffinity labeling experiments. Photoaffinity probes consisting of a side-chain portion of aplyronine A as a ligand, a diazirine moiety as a photoaffinity group, and a fluorophore as a detecting group were synthesized. Photolabeling experiments between actin and the probe were carried out. Actin was successfully photolabeled by the fluorescent probe and visualized clearly. The present results provide the first chemical evidence for the direct interaction between actin and the side-chain portion of aplyronine A.

Original languageEnglish
Pages (from-to)524-529
Number of pages6
JournalBioconjugate Chemistry
Volume17
Issue number2
DOIs
Publication statusPublished - Mar 2006
Externally publishedYes

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Fluorescent Dyes
Actins
Fluorophores
Labeling
Diazomethane
Experiments
Ligands
aplyronine A

ASJC Scopus subject areas

  • Chemistry(all)
  • Organic Chemistry
  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Study of the interaction between actin and antitumor substance aplyronine A with a novel fluorescent photoaffinity probe. / Kuroda, Takeshi; Suenaga, Kiyotake; Sakakura, Akira; Handa, Tomohisa; Okamoto, Kazuhito; Kigoshi, Hideo.

In: Bioconjugate Chemistry, Vol. 17, No. 2, 03.2006, p. 524-529.

Research output: Contribution to journalArticle

Kuroda, Takeshi ; Suenaga, Kiyotake ; Sakakura, Akira ; Handa, Tomohisa ; Okamoto, Kazuhito ; Kigoshi, Hideo. / Study of the interaction between actin and antitumor substance aplyronine A with a novel fluorescent photoaffinity probe. In: Bioconjugate Chemistry. 2006 ; Vol. 17, No. 2. pp. 524-529.
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