Study of Legionella pneumophila detection by two step polymerase chain reaction

M. Koide, A. Saito, F. Higa, Y. Yamashiro, T. Ishimine, M. Futenma, J. Inadome, K. Kawakami, Nobuchika Kusano

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We studied the usefulness of two sets of primers in macrophage infectivity potentiator gene sequence of Legionella pneumophila. PCR by 1st step primers produced 649 bp DNA bands and 2nd step primers 489 bp DNA bands. Two step PCR by these primers produced 489 bp DNA bands specific for L. pneumophila. Two step PCR detected 10 fg of pure DNA extracted from L. pneumophila, Philadelphia-1 strain. PCR sensitivity by these primers was superior to former primers reported by us using intratracheal aspirates collected from the patient with L. pneumophila serogroup 2 pneumonia.

Original languageEnglish
Pages (from-to)1062-1067
Number of pages6
JournalKansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases
Volume67
Issue number11
Publication statusPublished - Nov 1993
Externally publishedYes

Fingerprint

Legionella pneumophila
Polymerase Chain Reaction
DNA
Pneumonia
Macrophages
Genes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Study of Legionella pneumophila detection by two step polymerase chain reaction. / Koide, M.; Saito, A.; Higa, F.; Yamashiro, Y.; Ishimine, T.; Futenma, M.; Inadome, J.; Kawakami, K.; Kusano, Nobuchika.

In: Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases, Vol. 67, No. 11, 11.1993, p. 1062-1067.

Research output: Contribution to journalArticle

Koide, M, Saito, A, Higa, F, Yamashiro, Y, Ishimine, T, Futenma, M, Inadome, J, Kawakami, K & Kusano, N 1993, 'Study of Legionella pneumophila detection by two step polymerase chain reaction', Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases, vol. 67, no. 11, pp. 1062-1067.
Koide, M. ; Saito, A. ; Higa, F. ; Yamashiro, Y. ; Ishimine, T. ; Futenma, M. ; Inadome, J. ; Kawakami, K. ; Kusano, Nobuchika. / Study of Legionella pneumophila detection by two step polymerase chain reaction. In: Kansenshogaku zasshi. The Journal of the Japanese Association for Infectious Diseases. 1993 ; Vol. 67, No. 11. pp. 1062-1067.
@article{d24f66d679a04005905a96fc74ecfea6,
title = "Study of Legionella pneumophila detection by two step polymerase chain reaction",
abstract = "We studied the usefulness of two sets of primers in macrophage infectivity potentiator gene sequence of Legionella pneumophila. PCR by 1st step primers produced 649 bp DNA bands and 2nd step primers 489 bp DNA bands. Two step PCR by these primers produced 489 bp DNA bands specific for L. pneumophila. Two step PCR detected 10 fg of pure DNA extracted from L. pneumophila, Philadelphia-1 strain. PCR sensitivity by these primers was superior to former primers reported by us using intratracheal aspirates collected from the patient with L. pneumophila serogroup 2 pneumonia.",
author = "M. Koide and A. Saito and F. Higa and Y. Yamashiro and T. Ishimine and M. Futenma and J. Inadome and K. Kawakami and Nobuchika Kusano",
year = "1993",
month = "11",
language = "English",
volume = "67",
pages = "1062--1067",
journal = "Nippon Densenbyo Gakkai zasshi",
issn = "0387-5911",
publisher = "Nihon Kansensho Gakkai",
number = "11",

}

TY - JOUR

T1 - Study of Legionella pneumophila detection by two step polymerase chain reaction

AU - Koide, M.

AU - Saito, A.

AU - Higa, F.

AU - Yamashiro, Y.

AU - Ishimine, T.

AU - Futenma, M.

AU - Inadome, J.

AU - Kawakami, K.

AU - Kusano, Nobuchika

PY - 1993/11

Y1 - 1993/11

N2 - We studied the usefulness of two sets of primers in macrophage infectivity potentiator gene sequence of Legionella pneumophila. PCR by 1st step primers produced 649 bp DNA bands and 2nd step primers 489 bp DNA bands. Two step PCR by these primers produced 489 bp DNA bands specific for L. pneumophila. Two step PCR detected 10 fg of pure DNA extracted from L. pneumophila, Philadelphia-1 strain. PCR sensitivity by these primers was superior to former primers reported by us using intratracheal aspirates collected from the patient with L. pneumophila serogroup 2 pneumonia.

AB - We studied the usefulness of two sets of primers in macrophage infectivity potentiator gene sequence of Legionella pneumophila. PCR by 1st step primers produced 649 bp DNA bands and 2nd step primers 489 bp DNA bands. Two step PCR by these primers produced 489 bp DNA bands specific for L. pneumophila. Two step PCR detected 10 fg of pure DNA extracted from L. pneumophila, Philadelphia-1 strain. PCR sensitivity by these primers was superior to former primers reported by us using intratracheal aspirates collected from the patient with L. pneumophila serogroup 2 pneumonia.

UR - http://www.scopus.com/inward/record.url?scp=0027690622&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027690622&partnerID=8YFLogxK

M3 - Article

C2 - 8270798

AN - SCOPUS:0027690622

VL - 67

SP - 1062

EP - 1067

JO - Nippon Densenbyo Gakkai zasshi

JF - Nippon Densenbyo Gakkai zasshi

SN - 0387-5911

IS - 11

ER -