Studies on the regulatory mechanism of Staphylococcus aureus virulence

Research output: Contribution to journalReview article

1 Citation (Scopus)

Abstract

Staphylococcus aureus causes various diseases against humans, including skin infection, pneumonia, food poisoning, and meningitis. Methicillin-resistant S. aureus (MRSA) is resistant to a broad range of antibiotics, causing serious clinical problems. In this review, I summarize our studies to evaluate S. aureus virulence and identify novel virulence regulators. First, we utilized silkworms as an infection model of S. aureus and identified novel virulence factors of S. aureus. Some of the virulence factors interact with RNA in bacterial cells and regulate the expression of virulence factors. Second, we found that S. aureus cells spread on soft agar plates and form a giant colony. We call this phenomenon colony-spreading. High virulence community-acquired MRSA exhibits higher colony-spreading activity than hospital-associated MRSA. The difference in colony spreading is attributed to a specific gene in the mobile genetic element SCCmec carried by hospital-associated MRSA. The gene transcription product inhibits translation of a master regulator against S. aureus virulence genes, resulting in the attenuation of colony-spreading, exotoxin production, and animal killing ability.

Original languageEnglish
Pages (from-to)491-501
Number of pages11
JournalNihon saikingaku zasshi. Japanese journal of bacteriology
Volume69
Issue number3
DOIs
Publication statusPublished - Jan 1 2014
Externally publishedYes

Fingerprint

Virulence
Staphylococcus aureus
Methicillin-Resistant Staphylococcus aureus
Virulence Factors
Bacterial RNA
Interspersed Repetitive Sequences
Genes
Exotoxins
Foodborne Diseases
Bombyx
Infection
Meningitis
Agar
Pneumonia
Anti-Bacterial Agents
Skin

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Studies on the regulatory mechanism of Staphylococcus aureus virulence. / Kaito, Chikara.

In: Nihon saikingaku zasshi. Japanese journal of bacteriology, Vol. 69, No. 3, 01.01.2014, p. 491-501.

Research output: Contribution to journalReview article

@article{407d9031f95a42729da2ca4dce5a3775,
title = "Studies on the regulatory mechanism of Staphylococcus aureus virulence",
abstract = "Staphylococcus aureus causes various diseases against humans, including skin infection, pneumonia, food poisoning, and meningitis. Methicillin-resistant S. aureus (MRSA) is resistant to a broad range of antibiotics, causing serious clinical problems. In this review, I summarize our studies to evaluate S. aureus virulence and identify novel virulence regulators. First, we utilized silkworms as an infection model of S. aureus and identified novel virulence factors of S. aureus. Some of the virulence factors interact with RNA in bacterial cells and regulate the expression of virulence factors. Second, we found that S. aureus cells spread on soft agar plates and form a giant colony. We call this phenomenon colony-spreading. High virulence community-acquired MRSA exhibits higher colony-spreading activity than hospital-associated MRSA. The difference in colony spreading is attributed to a specific gene in the mobile genetic element SCCmec carried by hospital-associated MRSA. The gene transcription product inhibits translation of a master regulator against S. aureus virulence genes, resulting in the attenuation of colony-spreading, exotoxin production, and animal killing ability.",
author = "Chikara Kaito",
year = "2014",
month = "1",
day = "1",
doi = "10.3412/jsb.69.491",
language = "English",
volume = "69",
pages = "491--501",
journal = "Japanese Journal of Bacteriology",
issn = "0021-4930",
publisher = "Business Center for Academic Societies Japan/Nihon Gakkai Jimu Senta",
number = "3",

}

TY - JOUR

T1 - Studies on the regulatory mechanism of Staphylococcus aureus virulence

AU - Kaito, Chikara

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Staphylococcus aureus causes various diseases against humans, including skin infection, pneumonia, food poisoning, and meningitis. Methicillin-resistant S. aureus (MRSA) is resistant to a broad range of antibiotics, causing serious clinical problems. In this review, I summarize our studies to evaluate S. aureus virulence and identify novel virulence regulators. First, we utilized silkworms as an infection model of S. aureus and identified novel virulence factors of S. aureus. Some of the virulence factors interact with RNA in bacterial cells and regulate the expression of virulence factors. Second, we found that S. aureus cells spread on soft agar plates and form a giant colony. We call this phenomenon colony-spreading. High virulence community-acquired MRSA exhibits higher colony-spreading activity than hospital-associated MRSA. The difference in colony spreading is attributed to a specific gene in the mobile genetic element SCCmec carried by hospital-associated MRSA. The gene transcription product inhibits translation of a master regulator against S. aureus virulence genes, resulting in the attenuation of colony-spreading, exotoxin production, and animal killing ability.

AB - Staphylococcus aureus causes various diseases against humans, including skin infection, pneumonia, food poisoning, and meningitis. Methicillin-resistant S. aureus (MRSA) is resistant to a broad range of antibiotics, causing serious clinical problems. In this review, I summarize our studies to evaluate S. aureus virulence and identify novel virulence regulators. First, we utilized silkworms as an infection model of S. aureus and identified novel virulence factors of S. aureus. Some of the virulence factors interact with RNA in bacterial cells and regulate the expression of virulence factors. Second, we found that S. aureus cells spread on soft agar plates and form a giant colony. We call this phenomenon colony-spreading. High virulence community-acquired MRSA exhibits higher colony-spreading activity than hospital-associated MRSA. The difference in colony spreading is attributed to a specific gene in the mobile genetic element SCCmec carried by hospital-associated MRSA. The gene transcription product inhibits translation of a master regulator against S. aureus virulence genes, resulting in the attenuation of colony-spreading, exotoxin production, and animal killing ability.

UR - http://www.scopus.com/inward/record.url?scp=84929518122&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84929518122&partnerID=8YFLogxK

U2 - 10.3412/jsb.69.491

DO - 10.3412/jsb.69.491

M3 - Review article

C2 - 25186640

AN - SCOPUS:84929518122

VL - 69

SP - 491

EP - 501

JO - Japanese Journal of Bacteriology

JF - Japanese Journal of Bacteriology

SN - 0021-4930

IS - 3

ER -