Studies on the Glomerular Permeability with Human Liver Ferritin

Isao Kumagai, Hirofumi Makino, Zensuke Ota

Research output: Contribution to journalArticle

Abstract

To clarify the roles of the glomerular basement membrane (GBM) in filtration mechanisms, human liver ferritin was used for the first time as a tracer. Urinary excretion of human liver ferritin was measured and the injected ferritin was tracked under electron microscopy. Puromycin aminonucleoside (PAN) nephrosis was induced in Sprague Dawley rats and normal saline was injected into control rats. Monomeric and polymeric human ferritin were isolated from post mortem samples. Both kinds of human liver ferritin were injected into the experimental and control rats and urine samples were examined for human ferritin by radioimmunoassay. Rats with PAN nephrosis excreted approximately 33 times more monomeric ferritin than the controls. Appreciably more monomeric ferritin was excreted than polymeric ferritin. In control rats, monomeric ferritin particles were restricted in the lamina rara interna and inner aspect of the glomerular basement membrane 30 min after injection. On the other hand, in rats with PAN nephrosis, monomeric ferritin particles were seen throughout the width of the GBM and in the epithelial cells. With human liver ferritin, we were able to demonstrate the escape of the ferritin into the urine in addition to conducting the conventional electron microscopic tracer study of the glomerular capillary wall. Human liver ferritin shows potential as a useful tracer in the study of glomerular permselectivity.

Original languageEnglish
Pages (from-to)1221-1226
Number of pages6
JournalJapanese Journal of Nephrology
Volume35
Issue number11
DOIs
Publication statusPublished - Jan 1 1993

Fingerprint

Ferritins
Permeability
Liver
Puromycin Aminonucleoside
Nephrosis
Glomerular Basement Membrane
Urine
Radioimmunoassay
Sprague Dawley Rats
Electron Microscopy
Epithelial Cells

Keywords

  • ferritin
  • glomerular basement membrane
  • glomerular permeability
  • proteinuria

ASJC Scopus subject areas

  • Nephrology

Cite this

Studies on the Glomerular Permeability with Human Liver Ferritin. / Kumagai, Isao; Makino, Hirofumi; Ota, Zensuke.

In: Japanese Journal of Nephrology, Vol. 35, No. 11, 01.01.1993, p. 1221-1226.

Research output: Contribution to journalArticle

Kumagai, Isao ; Makino, Hirofumi ; Ota, Zensuke. / Studies on the Glomerular Permeability with Human Liver Ferritin. In: Japanese Journal of Nephrology. 1993 ; Vol. 35, No. 11. pp. 1221-1226.
@article{1fc7c5ceddbe46bf968bed0f1a2b668a,
title = "Studies on the Glomerular Permeability with Human Liver Ferritin",
abstract = "To clarify the roles of the glomerular basement membrane (GBM) in filtration mechanisms, human liver ferritin was used for the first time as a tracer. Urinary excretion of human liver ferritin was measured and the injected ferritin was tracked under electron microscopy. Puromycin aminonucleoside (PAN) nephrosis was induced in Sprague Dawley rats and normal saline was injected into control rats. Monomeric and polymeric human ferritin were isolated from post mortem samples. Both kinds of human liver ferritin were injected into the experimental and control rats and urine samples were examined for human ferritin by radioimmunoassay. Rats with PAN nephrosis excreted approximately 33 times more monomeric ferritin than the controls. Appreciably more monomeric ferritin was excreted than polymeric ferritin. In control rats, monomeric ferritin particles were restricted in the lamina rara interna and inner aspect of the glomerular basement membrane 30 min after injection. On the other hand, in rats with PAN nephrosis, monomeric ferritin particles were seen throughout the width of the GBM and in the epithelial cells. With human liver ferritin, we were able to demonstrate the escape of the ferritin into the urine in addition to conducting the conventional electron microscopic tracer study of the glomerular capillary wall. Human liver ferritin shows potential as a useful tracer in the study of glomerular permselectivity.",
keywords = "ferritin, glomerular basement membrane, glomerular permeability, proteinuria",
author = "Isao Kumagai and Hirofumi Makino and Zensuke Ota",
year = "1993",
month = "1",
day = "1",
doi = "10.14842/jpnjnephrol1959.35.1221",
language = "English",
volume = "35",
pages = "1221--1226",
journal = "Japanese Journal of Nephrology",
issn = "0385-2385",
publisher = "Japanese Society of Nephrology",
number = "11",

}

TY - JOUR

T1 - Studies on the Glomerular Permeability with Human Liver Ferritin

AU - Kumagai, Isao

AU - Makino, Hirofumi

AU - Ota, Zensuke

PY - 1993/1/1

Y1 - 1993/1/1

N2 - To clarify the roles of the glomerular basement membrane (GBM) in filtration mechanisms, human liver ferritin was used for the first time as a tracer. Urinary excretion of human liver ferritin was measured and the injected ferritin was tracked under electron microscopy. Puromycin aminonucleoside (PAN) nephrosis was induced in Sprague Dawley rats and normal saline was injected into control rats. Monomeric and polymeric human ferritin were isolated from post mortem samples. Both kinds of human liver ferritin were injected into the experimental and control rats and urine samples were examined for human ferritin by radioimmunoassay. Rats with PAN nephrosis excreted approximately 33 times more monomeric ferritin than the controls. Appreciably more monomeric ferritin was excreted than polymeric ferritin. In control rats, monomeric ferritin particles were restricted in the lamina rara interna and inner aspect of the glomerular basement membrane 30 min after injection. On the other hand, in rats with PAN nephrosis, monomeric ferritin particles were seen throughout the width of the GBM and in the epithelial cells. With human liver ferritin, we were able to demonstrate the escape of the ferritin into the urine in addition to conducting the conventional electron microscopic tracer study of the glomerular capillary wall. Human liver ferritin shows potential as a useful tracer in the study of glomerular permselectivity.

AB - To clarify the roles of the glomerular basement membrane (GBM) in filtration mechanisms, human liver ferritin was used for the first time as a tracer. Urinary excretion of human liver ferritin was measured and the injected ferritin was tracked under electron microscopy. Puromycin aminonucleoside (PAN) nephrosis was induced in Sprague Dawley rats and normal saline was injected into control rats. Monomeric and polymeric human ferritin were isolated from post mortem samples. Both kinds of human liver ferritin were injected into the experimental and control rats and urine samples were examined for human ferritin by radioimmunoassay. Rats with PAN nephrosis excreted approximately 33 times more monomeric ferritin than the controls. Appreciably more monomeric ferritin was excreted than polymeric ferritin. In control rats, monomeric ferritin particles were restricted in the lamina rara interna and inner aspect of the glomerular basement membrane 30 min after injection. On the other hand, in rats with PAN nephrosis, monomeric ferritin particles were seen throughout the width of the GBM and in the epithelial cells. With human liver ferritin, we were able to demonstrate the escape of the ferritin into the urine in addition to conducting the conventional electron microscopic tracer study of the glomerular capillary wall. Human liver ferritin shows potential as a useful tracer in the study of glomerular permselectivity.

KW - ferritin

KW - glomerular basement membrane

KW - glomerular permeability

KW - proteinuria

UR - http://www.scopus.com/inward/record.url?scp=0027762658&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027762658&partnerID=8YFLogxK

U2 - 10.14842/jpnjnephrol1959.35.1221

DO - 10.14842/jpnjnephrol1959.35.1221

M3 - Article

C2 - 8139133

AN - SCOPUS:0027762658

VL - 35

SP - 1221

EP - 1226

JO - Japanese Journal of Nephrology

JF - Japanese Journal of Nephrology

SN - 0385-2385

IS - 11

ER -