Studies on hemolytic action of a hemolysin produced by Vibrio mimicus

S. Shinoda, K. Ishida, E. G. Oh, K. Sasahara, Shin-ichi Miyoshi, M. A R Chowdhury, T. Yasuda

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Some properties and mechanism of action of a hemolysin (VMH) produced by an enteropathogenic Vibrio mimicus strain was examined. VMH was heat-labile and inhibited by addition of divalent cations, including Ca2+, Mg2+ and Mn2+. The hemolysis by VMH was inhibited by incubating with gangliosides, suggesting that the ganglioside was the binding site on the erythrocyte membrane for VMH. Existence of a galactose moiety on reducing end of the ganglioside molecule and a sialic acid on the galactose moiety was suggested to be important for the binding of VMH molecule. Colloid osmotic manner of the hemolysis by VMH was demonstrated.

Original languageEnglish
Pages (from-to)405-409
Number of pages5
JournalMicrobiology and Immunology
Volume37
Issue number5
Publication statusPublished - 1993

Fingerprint

Gangliosides
Hemolysis
Galactose
Vibrio mimicus
Hemolysin Proteins
Divalent Cations
Erythrocyte Membrane
Colloids
N-Acetylneuraminic Acid
Hot Temperature
Binding Sites
Vibrio hemolysin

Keywords

  • gangliosides
  • hemolysin
  • Vibrio mimicus

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Microbiology
  • Microbiology (medical)

Cite this

Shinoda, S., Ishida, K., Oh, E. G., Sasahara, K., Miyoshi, S., Chowdhury, M. A. R., & Yasuda, T. (1993). Studies on hemolytic action of a hemolysin produced by Vibrio mimicus. Microbiology and Immunology, 37(5), 405-409.

Studies on hemolytic action of a hemolysin produced by Vibrio mimicus. / Shinoda, S.; Ishida, K.; Oh, E. G.; Sasahara, K.; Miyoshi, Shin-ichi; Chowdhury, M. A R; Yasuda, T.

In: Microbiology and Immunology, Vol. 37, No. 5, 1993, p. 405-409.

Research output: Contribution to journalArticle

Shinoda, S, Ishida, K, Oh, EG, Sasahara, K, Miyoshi, S, Chowdhury, MAR & Yasuda, T 1993, 'Studies on hemolytic action of a hemolysin produced by Vibrio mimicus', Microbiology and Immunology, vol. 37, no. 5, pp. 405-409.
Shinoda S, Ishida K, Oh EG, Sasahara K, Miyoshi S, Chowdhury MAR et al. Studies on hemolytic action of a hemolysin produced by Vibrio mimicus. Microbiology and Immunology. 1993;37(5):405-409.
Shinoda, S. ; Ishida, K. ; Oh, E. G. ; Sasahara, K. ; Miyoshi, Shin-ichi ; Chowdhury, M. A R ; Yasuda, T. / Studies on hemolytic action of a hemolysin produced by Vibrio mimicus. In: Microbiology and Immunology. 1993 ; Vol. 37, No. 5. pp. 405-409.
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