Studies on glutathione S-transferase gene involved in chlorfluazuron resistance of the diamondback moth, Plutella xylostella L. (Lepidoptera: Yponomeutidae)

Shoji Sonoda, Hisaaki Tsumuki

    Research output: Contribution to journalArticlepeer-review

    43 Citations (Scopus)

    Abstract

    Relationship between GST-3 and chlorfluazuron resistance was studied using the non-selected (NS) and chlorfluazuron-resistant (CFR) strains of the diamondback moth (DBM), Plutella xylostella L. The CFR strain exhibited higher GST-3 gene expression and GST activity compared to the NS strain. These results suggest that GST-3 is involved in detoxification of chlorfluazuron in DBM. Southern blot and nucleotide sequencing analyses showed that the higher GST-3 expression in the CFR strain may result from enhanced gene transcription rather than gene amplification or point mutations in the structural gene. Possible mechanisms of the higher GST-3 gene transcription in the CFR strain were discussed.

    Original languageEnglish
    Pages (from-to)94-101
    Number of pages8
    JournalPesticide Biochemistry and Physiology
    Volume82
    Issue number1
    DOIs
    Publication statusPublished - May 2005

    Keywords

    • Chlorfluazuron
    • Diamondback moth
    • Glutathione S-transferase
    • Insecticide resistance
    • Plutella xylostella L.

    ASJC Scopus subject areas

    • Agronomy and Crop Science
    • Health, Toxicology and Mutagenesis

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