Relationship between GST-3 and chlorfluazuron resistance was studied using the non-selected (NS) and chlorfluazuron-resistant (CFR) strains of the diamondback moth (DBM), Plutella xylostella L. The CFR strain exhibited higher GST-3 gene expression and GST activity compared to the NS strain. These results suggest that GST-3 is involved in detoxification of chlorfluazuron in DBM. Southern blot and nucleotide sequencing analyses showed that the higher GST-3 expression in the CFR strain may result from enhanced gene transcription rather than gene amplification or point mutations in the structural gene. Possible mechanisms of the higher GST-3 gene transcription in the CFR strain were discussed.
|Number of pages||8|
|Journal||Pesticide Biochemistry and Physiology|
|Publication status||Published - May 2005|
- Diamondback moth
- Glutathione S-transferase
- Insecticide resistance
- Plutella xylostella L.
ASJC Scopus subject areas
- Agronomy and Crop Science
- Health, Toxicology and Mutagenesis