Studies on glutathione S-transferase gene involved in chlorfluazuron resistance of the diamondback moth, Plutella xylostella L. (Lepidoptera: Yponomeutidae)

Shoji Sonoda, Hisaaki Tsumuki

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Relationship between GST-3 and chlorfluazuron resistance was studied using the non-selected (NS) and chlorfluazuron-resistant (CFR) strains of the diamondback moth (DBM), Plutella xylostella L. The CFR strain exhibited higher GST-3 gene expression and GST activity compared to the NS strain. These results suggest that GST-3 is involved in detoxification of chlorfluazuron in DBM. Southern blot and nucleotide sequencing analyses showed that the higher GST-3 expression in the CFR strain may result from enhanced gene transcription rather than gene amplification or point mutations in the structural gene. Possible mechanisms of the higher GST-3 gene transcription in the CFR strain were discussed.

Original languageEnglish
Pages (from-to)94-101
Number of pages8
JournalPesticide Biochemistry and Physiology
Volume82
Issue number1
DOIs
Publication statusPublished - May 2005

Fingerprint

chlorfluazuron
Yponomeutidae
Lepidoptera
Moths
Plutella xylostella
Glutathione Transferase
glutathione transferase
Genes
genes
Transcription
transcription (genetics)
gene amplification
Detoxification
Gene Amplification
structural genes
point mutation
Southern Blotting
Point Mutation
Gene expression
Southern blotting

Keywords

  • Chlorfluazuron
  • Diamondback moth
  • Glutathione S-transferase
  • Insecticide resistance
  • Plutella xylostella L.

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Biochemistry
  • Physiology

Cite this

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title = "Studies on glutathione S-transferase gene involved in chlorfluazuron resistance of the diamondback moth, Plutella xylostella L. (Lepidoptera: Yponomeutidae)",
abstract = "Relationship between GST-3 and chlorfluazuron resistance was studied using the non-selected (NS) and chlorfluazuron-resistant (CFR) strains of the diamondback moth (DBM), Plutella xylostella L. The CFR strain exhibited higher GST-3 gene expression and GST activity compared to the NS strain. These results suggest that GST-3 is involved in detoxification of chlorfluazuron in DBM. Southern blot and nucleotide sequencing analyses showed that the higher GST-3 expression in the CFR strain may result from enhanced gene transcription rather than gene amplification or point mutations in the structural gene. Possible mechanisms of the higher GST-3 gene transcription in the CFR strain were discussed.",
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AB - Relationship between GST-3 and chlorfluazuron resistance was studied using the non-selected (NS) and chlorfluazuron-resistant (CFR) strains of the diamondback moth (DBM), Plutella xylostella L. The CFR strain exhibited higher GST-3 gene expression and GST activity compared to the NS strain. These results suggest that GST-3 is involved in detoxification of chlorfluazuron in DBM. Southern blot and nucleotide sequencing analyses showed that the higher GST-3 expression in the CFR strain may result from enhanced gene transcription rather than gene amplification or point mutations in the structural gene. Possible mechanisms of the higher GST-3 gene transcription in the CFR strain were discussed.

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