Studies on glutathione S-transferase gene involved in chlorfluazuron resistance of the diamondback moth, Plutella xylostella L. (Lepidoptera: Yponomeutidae)

Shoji Sonoda, Hisaaki Tsumuki

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Relationship between GST-3 and chlorfluazuron resistance was studied using the non-selected (NS) and chlorfluazuron-resistant (CFR) strains of the diamondback moth (DBM), Plutella xylostella L. The CFR strain exhibited higher GST-3 gene expression and GST activity compared to the NS strain. These results suggest that GST-3 is involved in detoxification of chlorfluazuron in DBM. Southern blot and nucleotide sequencing analyses showed that the higher GST-3 expression in the CFR strain may result from enhanced gene transcription rather than gene amplification or point mutations in the structural gene. Possible mechanisms of the higher GST-3 gene transcription in the CFR strain were discussed.

Original languageEnglish
Pages (from-to)94-101
Number of pages8
JournalPesticide Biochemistry and Physiology
Volume82
Issue number1
DOIs
Publication statusPublished - May 2005

Keywords

  • Chlorfluazuron
  • Diamondback moth
  • Glutathione S-transferase
  • Insecticide resistance
  • Plutella xylostella L.

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Health, Toxicology and Mutagenesis

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