Studies on diarrheagenic Escherichia coli isolated from children with diarrhea in Myanmar

Eizo Takahashi, Zafar Sultan, Sumika Shimada, Wah Wah Aung, Mar Mar Nyein, Khin Nwe Oo, Gopinath Balakrish Nair, Yoshifumi Takeda, Keinosuke Okamoto

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Escherichia coli isolates from 217 children in Myanmar with diarrhea were investigated for the presence of virulence genes related to diarrhea by colony hybridization and PCR. The genes examined were lt, stI, stII, stx1, stx2, eae, bfp, pCVD (which is the representative gene of plasmid of pCVD of EAEC), and ial (which is invasion-associated locus of the invasion plasmid found in EIEC). Isolates from 47 of 217 children (21.7%) possessed virulence genes characteristic of diarrheagenic E. coli. No instance was found of co-existence of different E. coli strains with different virulence genes in the same patient. Diarrheagenic E. coli are currently classified into five categories based on their virulence markers: ETEC, EHEC, EPEC, EAEC, and EIEC. Of the 47 isolates examined, 30 were EAEC, 12 were EPEC and 5 were ETEC. Susceptibility tests for antimicrobial agents showed that almost all diarrheagenic isolates were resistant to penicillin, tetracycline and streptomycin. However, the majority of strains were sensitive to cephalexin, nalidixic acid and norfloxacin. In particular, 42 of the 47 isolates were sensitive to norfloxacin, which is a fluoroquinolone. This study shows EAEC and EPEC are responsible for sporadic diarrhea in Myanmar and fluoroquinolones appear to be effective in the treatment of these patients.

Original languageEnglish
Pages (from-to)2-8
Number of pages7
JournalMicrobiology and Immunology
Volume52
Issue number1
DOIs
Publication statusPublished - Jan 2008

Fingerprint

Myanmar
Diarrhea
Enteropathogenic Escherichia coli
Virulence
Escherichia coli
Enterotoxigenic Escherichia coli
Norfloxacin
Genes
Fluoroquinolones
Plasmids
Enterohemorrhagic Escherichia coli
Cephalexin
Nalidixic Acid
Streptomycin
Anti-Infective Agents
Tetracycline
Penicillins
Polymerase Chain Reaction

Keywords

  • Children
  • Diarrhea
  • E. Coli
  • Myanmar

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Immunology and Microbiology(all)
  • Microbiology (medical)

Cite this

Studies on diarrheagenic Escherichia coli isolated from children with diarrhea in Myanmar. / Takahashi, Eizo; Sultan, Zafar; Shimada, Sumika; Aung, Wah Wah; Nyein, Mar Mar; Oo, Khin Nwe; Nair, Gopinath Balakrish; Takeda, Yoshifumi; Okamoto, Keinosuke.

In: Microbiology and Immunology, Vol. 52, No. 1, 01.2008, p. 2-8.

Research output: Contribution to journalArticle

Takahashi, Eizo ; Sultan, Zafar ; Shimada, Sumika ; Aung, Wah Wah ; Nyein, Mar Mar ; Oo, Khin Nwe ; Nair, Gopinath Balakrish ; Takeda, Yoshifumi ; Okamoto, Keinosuke. / Studies on diarrheagenic Escherichia coli isolated from children with diarrhea in Myanmar. In: Microbiology and Immunology. 2008 ; Vol. 52, No. 1. pp. 2-8.
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