Structures of sugar chains of water-soluble glycoproteins in developing castor bean cotyledons.

Yoshinobu Kimura, Hiroshi Suehisa, Osamu Yamaguchi, Shuhei Nakajima, Shigeaki Takagi

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The structures of sugar chains from the water-soluble glycoproteins in developing castor beans have been identified. The structural analyses were done by a fluorescence method combined with exoglycosidase digestions and 1H-NMR spectroscopy. The identified structures fell into two categories; one was an oligomannose-type, the other xylomannose-type or xylose-containing type. Among these oligosaccharides, Man3Fuc1Xyl1GlcNAc2 (M3FX; 38%) and Man6GlcNAc2 (M6B; 22%) were the major structures. The higher mannose-content oligosaccharides (Man8-7GlcNAc2) were only 4.1%, and the further-modified structures (GNM3FX, M2FX) than M3FX were 22% of the total.

Original languageEnglish
Pages (from-to)3259-3267
Number of pages9
JournalAgricultural and Biological Chemistry
Volume54
Issue number12
DOIs
Publication statusPublished - Dec 1990

Fingerprint

Castor Bean
castor beans
Cotyledon
Oligosaccharides
oligosaccharides
Sugars
cotyledons
glycoproteins
Glycoproteins
sugars
Water
glycosidases
Glycoside Hydrolases
Xylose
Mannose
mannose
xylose
Nuclear magnetic resonance spectroscopy
nuclear magnetic resonance spectroscopy
Digestion

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Structures of sugar chains of water-soluble glycoproteins in developing castor bean cotyledons. / Kimura, Yoshinobu; Suehisa, Hiroshi; Yamaguchi, Osamu; Nakajima, Shuhei; Takagi, Shigeaki.

In: Agricultural and Biological Chemistry, Vol. 54, No. 12, 12.1990, p. 3259-3267.

Research output: Contribution to journalArticle

Kimura, Yoshinobu ; Suehisa, Hiroshi ; Yamaguchi, Osamu ; Nakajima, Shuhei ; Takagi, Shigeaki. / Structures of sugar chains of water-soluble glycoproteins in developing castor bean cotyledons. In: Agricultural and Biological Chemistry. 1990 ; Vol. 54, No. 12. pp. 3259-3267.
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