Structures of sugar chains of Ricinus communis agglutinin

Yoshinobu Kimura, Sumihiro Hase, Yuji Kobayashi, Yoshimasa Kyogoku, Tokuji Ikenaka, Gunki Funatsu

Research output: Contribution to journalArticlepeer-review

34 Citations (Scopus)

Abstract

The structures of sugar chains from Ricinus communis agglutinin were determined. Four glycopeptides were isolated from the lectin according to a published method (Kimura, Y. and Funatsu, G. (1988) Agric. Biol. Chem. 52, in press), and sugar chains of each glycopeptide were liberated by hydrazinolysis. Free amino groups were N-acetylated and the reducing-end residues were coupled with 2-aminopyridine. The resulting pyridylamino derivatives of sugar chains were purified by gel filtration and reversed-phase HPLC. The structures of thus-purified PA-sugar chains were determined by a combination of component analysis, stepwise exoglycosidase digestions, partial acetolysis, and 500 MHz 1H-NMR spectroscopy. These results indicate that R. communis agglutinin contains the sugar chains shown on page 249.

Original languageEnglish
Pages (from-to)248-256
Number of pages9
JournalBBA - General Subjects
Volume966
Issue number2
DOIs
Publication statusPublished - Aug 11 1988
Externally publishedYes

Keywords

  • Glycoprotein
  • Lectin
  • Pyridylamino derivative
  • Ricinus communis agglutinin
  • Sugar chain processing
  • Sugar chain structure

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

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