Structures of sugar chains of Ricinus communis agglutinin

Yoshinobu Kimura, Sumihiro Hase, Yuji Kobayashi, Yoshimasa Kyogoku, Tokuji Ikenaka, Gunki Funatsu

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The structures of sugar chains from Ricinus communis agglutinin were determined. Four glycopeptides were isolated from the lectin according to a published method (Kimura, Y. and Funatsu, G. (1988) Agric. Biol. Chem. 52, in press), and sugar chains of each glycopeptide were liberated by hydrazinolysis. Free amino groups were N-acetylated and the reducing-end residues were coupled with 2-aminopyridine. The resulting pyridylamino derivatives of sugar chains were purified by gel filtration and reversed-phase HPLC. The structures of thus-purified PA-sugar chains were determined by a combination of component analysis, stepwise exoglycosidase digestions, partial acetolysis, and 500 MHz 1H-NMR spectroscopy. These results indicate that R. communis agglutinin contains the sugar chains shown on page 249.

Original languageEnglish
Pages (from-to)248-256
Number of pages9
JournalBiochimica et Biophysica Acta - General Subjects
Volume966
Issue number2
DOIs
Publication statusPublished - Aug 11 1988
Externally publishedYes

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Glycopeptides
Sugars
Glycoside Hydrolases
Lectins
Gel Chromatography
Digestion
Magnetic Resonance Spectroscopy
High Pressure Liquid Chromatography
Agglutinins
Nuclear magnetic resonance spectroscopy
Gels
Ricinus communis agglutinin-1
Derivatives
alpha-aminopyridine
Proton Magnetic Resonance Spectroscopy

Keywords

  • Glycoprotein
  • Lectin
  • Pyridylamino derivative
  • Ricinus communis agglutinin
  • Sugar chain processing
  • Sugar chain structure

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Structures of sugar chains of Ricinus communis agglutinin. / Kimura, Yoshinobu; Hase, Sumihiro; Kobayashi, Yuji; Kyogoku, Yoshimasa; Ikenaka, Tokuji; Funatsu, Gunki.

In: Biochimica et Biophysica Acta - General Subjects, Vol. 966, No. 2, 11.08.1988, p. 248-256.

Research output: Contribution to journalArticle

Kimura, Yoshinobu ; Hase, Sumihiro ; Kobayashi, Yuji ; Kyogoku, Yoshimasa ; Ikenaka, Tokuji ; Funatsu, Gunki. / Structures of sugar chains of Ricinus communis agglutinin. In: Biochimica et Biophysica Acta - General Subjects. 1988 ; Vol. 966, No. 2. pp. 248-256.
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