TY - JOUR
T1 - Structures of sugar chains of ricin D
AU - Kimura, Yoshinobu
AU - Hase, Sumihiro
AU - Kobayashi, Yuji
AU - Kyogoku, Yoshimasa
AU - Ikenaka, Tokuji
AU - Funatsu, Gunki
PY - 1988/6
Y1 - 1988/6
N2 - The toxic lectin, ricin D, contains mannose, fucose, xylose, and N-acetylglucosamine as sugar components. Sugar chains are linked to Asn-10 of the A-chain, and to Asn-95 and Asn-135 of the B-chain (Funatsu, G. et al. (1978) Agric. Biol. Chem. 42, 501-503; Araki, T.& Funatsu, G. (1985) FEBS Lett. 191, 121-124). Asparagine-linked sugar chains of each glycopeptide from ricin D were liberated by hydrazinolysis followed by N-acetylation. The reducing end residues of the sugar chains were coupled with 2-aminopyridine and the pyridylamino (PA-) derivatives obtained were purified by gel-filtration and reversed-phase HPLC. Eight main PA-sugar chains were obtained from three glycopeptides and the structures of these sugar chains were determined by component analysis, stepwise exoglycosidase digestions, partial acetolysis, and 500 MHZ 1H-NMR spectroscopy. The results show that oligomannose type sugar chains (Man6-7GlcNAc2) are linked to Asn-95; Man5-7 GlcNAc2 and M4X (structure, see below) to Asn-135 of the B-chain, and M3FX and M3X to Asn-10 of the A-chain.Manα13Manα1{set minus}M36anβ1-4GlcNAcβ1-4GlcNAcM4X Manα12{set minus}Xylβ1Manα1{set minus}M36anβ1-4GlcNAcβ1-4GlcNAcM3XManα12{set minus}Xylβ1Manα1{set minus}M36anβ1-4GlcNAcβ1-4GlcNAcM3FXManα12{set minus}3Xylβ1Fucα1
AB - The toxic lectin, ricin D, contains mannose, fucose, xylose, and N-acetylglucosamine as sugar components. Sugar chains are linked to Asn-10 of the A-chain, and to Asn-95 and Asn-135 of the B-chain (Funatsu, G. et al. (1978) Agric. Biol. Chem. 42, 501-503; Araki, T.& Funatsu, G. (1985) FEBS Lett. 191, 121-124). Asparagine-linked sugar chains of each glycopeptide from ricin D were liberated by hydrazinolysis followed by N-acetylation. The reducing end residues of the sugar chains were coupled with 2-aminopyridine and the pyridylamino (PA-) derivatives obtained were purified by gel-filtration and reversed-phase HPLC. Eight main PA-sugar chains were obtained from three glycopeptides and the structures of these sugar chains were determined by component analysis, stepwise exoglycosidase digestions, partial acetolysis, and 500 MHZ 1H-NMR spectroscopy. The results show that oligomannose type sugar chains (Man6-7GlcNAc2) are linked to Asn-95; Man5-7 GlcNAc2 and M4X (structure, see below) to Asn-135 of the B-chain, and M3FX and M3X to Asn-10 of the A-chain.Manα13Manα1{set minus}M36anβ1-4GlcNAcβ1-4GlcNAcM4X Manα12{set minus}Xylβ1Manα1{set minus}M36anβ1-4GlcNAcβ1-4GlcNAcM3XManα12{set minus}Xylβ1Manα1{set minus}M36anβ1-4GlcNAcβ1-4GlcNAcM3FXManα12{set minus}3Xylβ1Fucα1
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U2 - 10.1093/oxfordjournals.jbchem.a122391
DO - 10.1093/oxfordjournals.jbchem.a122391
M3 - Article
C2 - 3170523
AN - SCOPUS:0023927967
VL - 103
SP - 944
EP - 949
JO - Journal of Biochemistry
JF - Journal of Biochemistry
SN - 0021-924X
IS - 6
ER -