Structures of sugar chains of abrin a obtained from Abrus precatorius seeds

Yoshinobu Kimura, Sumihiro Hase, Tokuji Ikenaka, Gunki Funatsu

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The amino acid sequences around the glycosylation sites and the structures of the sugar chains of abrin a (a lectin derived from Abrus precatorius) were determined. Two glycopeptides were isolated from pronase digests of the B-chain of abrin a after gel filtration and ion-exchange chromatography. The amino acid sequences of these glycopeptides were determined to be Asp-Asn(CHO)-Gly-Thr and Gly-Asn(CHO)-Asn. The sugar chains of each glycopeptide were liberated by hydrazinolysis and then free amino groups were N-acetylated. The reducing-end residues were coupled with 2-aminopyridine and the resulting pyridylamino (PA-) derivatives of sugar chains were purified by reversed-phase HPLC. The structures of the purified PA-sugar chains were estimated by component analysis, comparison of elution positions with those of standard PA-sugar chains by two kinds of HPLC (Hase, S. et al. (1986) J. Biochem. 100, 1-100), stepwise exoglycosidase digestions, and partial acetolysis. Abrin a was shown to contain the following sugar chains. {A figure is presented}.

Original languageEnglish
Pages (from-to)150-159
Number of pages10
JournalBiochimica et Biophysica Acta - General Subjects
Volume966
Issue number1
DOIs
Publication statusPublished - Jul 14 1988
Externally publishedYes

Fingerprint

Abrin
Abrus
Glycopeptides
Sugars
Seed
Seeds
glycyl-threonine
Amino Acid Sequence
High Pressure Liquid Chromatography
Pronase
Glycoside Hydrolases
Ion Exchange Chromatography
Glycosylation
Lectins
Gel Chromatography
Digestion
Amino Acids
Chromatography
Ion exchange
Gels

Keywords

  • (A. precatorius)
  • Abrin
  • Glycopeptide isolation
  • Lectin
  • Sugar chain structure

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Structures of sugar chains of abrin a obtained from Abrus precatorius seeds. / Kimura, Yoshinobu; Hase, Sumihiro; Ikenaka, Tokuji; Funatsu, Gunki.

In: Biochimica et Biophysica Acta - General Subjects, Vol. 966, No. 1, 14.07.1988, p. 150-159.

Research output: Contribution to journalArticle

Kimura, Yoshinobu ; Hase, Sumihiro ; Ikenaka, Tokuji ; Funatsu, Gunki. / Structures of sugar chains of abrin a obtained from Abrus precatorius seeds. In: Biochimica et Biophysica Acta - General Subjects. 1988 ; Vol. 966, No. 1. pp. 150-159.
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