Structures of n-linked oligosaccharides of microsomal glycoproteins from developing castor bean endosperms

Yoshinobu Kimura, Yoshifumi Nakagawa, Toshihiko Tokuda, Masafumi Yamai, Shûhei Nakajlma, Eiji Higashide, Shigeaki Takagi

Research output: Contribution to journalArticle

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Abstract

The structures of sugar chains of the glycoproteins from the microsomal fraction of developing castor bean endosperms have been analyzed. The structural analyses were done by a fluorescence method combined with component analysis, exoglycosidase digestions, partial acetolysis, Smith degradation, and 1H-NMR spectroscopy. The estimated structures fell into three categories; the first was oligomannose-type, the second xylomannose-type, the third complex-type. Among these oligosaccharides, Man3Fuc1Xyl1GIcNAc2 (M3FX) and Man6GlcNAc2 (M6B) were the major structures. The structures of Man4GlcNAc2 (M4C) and Man4Xyl1 GIcNAc2 (M4X) have also been found in the microsomal glycoproteins of the developing bean endosperms. These results could indicate that the structures of M4C, M4X, and M3FX are formed in the stage of sugar chain processing in the microsomal fraction, in which oligomannose-type sugar chains are modified into complex-type ones by several kinds of processing enzymes.

Original languageEnglish
Pages (from-to)215-222
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume56
Issue number2
DOIs
Publication statusPublished - 1992

Fingerprint

Castor Bean
Endosperm
castor beans
Oligosaccharides
oligosaccharides
Sugars
endosperm
glycoproteins
Glycoproteins
sugars
Glycoside Hydrolases
Digestion
Magnetic Resonance Spectroscopy
glycosidases
Fluorescence
Processing
Nuclear magnetic resonance spectroscopy
nuclear magnetic resonance spectroscopy
beans
Enzymes

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Organic Chemistry
  • Analytical Chemistry
  • Applied Microbiology and Biotechnology
  • Food Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry (miscellaneous)

Cite this

Structures of n-linked oligosaccharides of microsomal glycoproteins from developing castor bean endosperms. / Kimura, Yoshinobu; Nakagawa, Yoshifumi; Tokuda, Toshihiko; Yamai, Masafumi; Nakajlma, Shûhei; Higashide, Eiji; Takagi, Shigeaki.

In: Bioscience, Biotechnology and Biochemistry, Vol. 56, No. 2, 1992, p. 215-222.

Research output: Contribution to journalArticle

Kimura, Yoshinobu ; Nakagawa, Yoshifumi ; Tokuda, Toshihiko ; Yamai, Masafumi ; Nakajlma, Shûhei ; Higashide, Eiji ; Takagi, Shigeaki. / Structures of n-linked oligosaccharides of microsomal glycoproteins from developing castor bean endosperms. In: Bioscience, Biotechnology and Biochemistry. 1992 ; Vol. 56, No. 2. pp. 215-222.
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