Abstract
The structures of N-linked sugar chains of glycoproteins expressed in tobacco BY2 cultured cells are reported. Five pyridylaminated (PA-) N-linked sugar chains were derived and purified from hydrazinolysates of the glycoproteins by reversed-phase HPLC and size-fractionation HPLC. The structures of the PA-sugar chains purified were identified by two-dimensional PA-sugar chain mapping, ion-spray MS/MS analysis, and exoglycosidase digestions. The five structures fell into two categories; the major class (92.5% as molar ratio) was a xylose containing-type (Man3Fuc1Xyl1GlcNAc2 (41.0%), GlcNAc2Man3Fuc1Xyl1GlcNAc2 (26.5%), GlcNAc1Man3Fuc1Xyl1GlcNAc2 (21.7%), Man3Xyl1GlcNAc2 (3.3%)), and the minor class was a high-mannose type (Man5GlcNAc2 (7.5%)). This is the first report to show that α(1→3) fucosylation of N-glycans does occur but β(1→4) galactosylation of the sugar chains does not in the tobacco cultured cells.
Original language | English |
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Pages (from-to) | 35-39 |
Number of pages | 5 |
Journal | Bioscience, Biotechnology and Biochemistry |
Volume | 63 |
Issue number | 1 |
DOIs | |
Publication status | Published - 1999 |
Keywords
- BY2 cell
- N-glycans
- Plant glycoprotein
- Sugar chain
- Tobacco cultured cell
ASJC Scopus subject areas
- Biotechnology
- Analytical Chemistry
- Biochemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
- Organic Chemistry