Abstract
The structures of N-linked sugar chains of glycoproteins expressed in tobacco BY2 cultured cells are reported. Five pyridylaminated (PA-) N-linked sugar chains were derived and purified from hydrazinolysates of the glycoproteins by reversed-phase HPLC and size-fractionation HPLC. The structures of the PA-sugar chains purified were identified by two-dimensional PA-sugar chain mapping, ion-spray MS/MS analysis, and exoglycosidase digestions. The five structures fell into two categories; the major class (92.5% as molar ratio) was a xylose containing-type (Man3Fuc1 Xyl1GlcNAc2 (41.0%), GlcNAc2Man3Fuc1Xyl1GlcNAc 2 (26.5%), GlcNAc1MaD3Fuc1Xyl1GlcNAc 2 (21.7%), Man3 Xyl1GlcNAc2 (3.3%)), and the minor class was a high-mannose type (Man5GlcNAc2 (7.5%)). This is the first report to show that α(1-→3) fucosylation of N-glycans does occur but β(1→4) galactosylation of the sugar chains does not in the tobacco cultured cells.
Original language | English |
---|---|
Pages (from-to) | 35-39 |
Number of pages | 5 |
Journal | Bioscience, Biotechnology and Biochemistry |
Volume | 63 |
Issue number | 1 |
Publication status | Published - Jan 1999 |
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Keywords
- BY2 cell
- N-glycans
- Plant glycoprotein
- Sugar chain
- Tobacco cultured cell
ASJC Scopus subject areas
- Food Science
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Biotechnology
- Chemistry (miscellaneous)
- Applied Microbiology and Biotechnology
- Bioengineering
Cite this
Structures of N-linked oligosaccharides of glycoproteins from tobacco BY2 suspension cultured cells. / Palacpac, Nirianne Q.; Kimura, Yoshinobu; Fujiyama, Kazuhito; Yoshida, Toshiomi; Seki, Tatsuji.
In: Bioscience, Biotechnology and Biochemistry, Vol. 63, No. 1, 01.1999, p. 35-39.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Structures of N-linked oligosaccharides of glycoproteins from tobacco BY2 suspension cultured cells
AU - Palacpac, Nirianne Q.
AU - Kimura, Yoshinobu
AU - Fujiyama, Kazuhito
AU - Yoshida, Toshiomi
AU - Seki, Tatsuji
PY - 1999/1
Y1 - 1999/1
N2 - The structures of N-linked sugar chains of glycoproteins expressed in tobacco BY2 cultured cells are reported. Five pyridylaminated (PA-) N-linked sugar chains were derived and purified from hydrazinolysates of the glycoproteins by reversed-phase HPLC and size-fractionation HPLC. The structures of the PA-sugar chains purified were identified by two-dimensional PA-sugar chain mapping, ion-spray MS/MS analysis, and exoglycosidase digestions. The five structures fell into two categories; the major class (92.5% as molar ratio) was a xylose containing-type (Man3Fuc1 Xyl1GlcNAc2 (41.0%), GlcNAc2Man3Fuc1Xyl1GlcNAc 2 (26.5%), GlcNAc1MaD3Fuc1Xyl1GlcNAc 2 (21.7%), Man3 Xyl1GlcNAc2 (3.3%)), and the minor class was a high-mannose type (Man5GlcNAc2 (7.5%)). This is the first report to show that α(1-→3) fucosylation of N-glycans does occur but β(1→4) galactosylation of the sugar chains does not in the tobacco cultured cells.
AB - The structures of N-linked sugar chains of glycoproteins expressed in tobacco BY2 cultured cells are reported. Five pyridylaminated (PA-) N-linked sugar chains were derived and purified from hydrazinolysates of the glycoproteins by reversed-phase HPLC and size-fractionation HPLC. The structures of the PA-sugar chains purified were identified by two-dimensional PA-sugar chain mapping, ion-spray MS/MS analysis, and exoglycosidase digestions. The five structures fell into two categories; the major class (92.5% as molar ratio) was a xylose containing-type (Man3Fuc1 Xyl1GlcNAc2 (41.0%), GlcNAc2Man3Fuc1Xyl1GlcNAc 2 (26.5%), GlcNAc1MaD3Fuc1Xyl1GlcNAc 2 (21.7%), Man3 Xyl1GlcNAc2 (3.3%)), and the minor class was a high-mannose type (Man5GlcNAc2 (7.5%)). This is the first report to show that α(1-→3) fucosylation of N-glycans does occur but β(1→4) galactosylation of the sugar chains does not in the tobacco cultured cells.
KW - BY2 cell
KW - N-glycans
KW - Plant glycoprotein
KW - Sugar chain
KW - Tobacco cultured cell
UR - http://www.scopus.com/inward/record.url?scp=0032602265&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032602265&partnerID=8YFLogxK
M3 - Article
C2 - 10052119
AN - SCOPUS:0032602265
VL - 63
SP - 35
EP - 39
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 1
ER -