Structures of N-Linked Oligosaccharides of Glycoproteins from Tobacco BY2 Suspension Cultured Cells

Nirianne Q. Palacpac, Yoshinobu Kimura, Kazuhito Fujiyama, Toshiomi Yoshida, Tatsuji Seki

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The structures of N-linked sugar chains of glycoproteins expressed in tobacco BY2 cultured cells are reported. Five pyridylaminated (PA-) N-linked sugar chains were derived and purified from hydrazinolysates of the glycoproteins by reversed-phase HPLC and size-fractionation HPLC. The structures of the PA-sugar chains purified were identified by two-dimensional PA-sugar chain mapping, ion-spray MS/MS analysis, and exoglycosidase digestions. The five structures fell into two categories; the major class (92.5% as molar ratio) was a xylose containing-type (Man3Fuc1Xyl1GlcNAc2 (41.0%), GlcNAc2Man3Fuc1Xyl1GlcNAc2 (26.5%), GlcNAc1Man3Fuc1Xyl1GlcNAc2 (21.7%), Man3Xyl1GlcNAc2 (3.3%)), and the minor class was a high-mannose type (Man5GlcNAc2 (7.5%)). This is the first report to show that α(1→3) fucosylation of N-glycans does occur but β(1→4) galactosylation of the sugar chains does not in the tobacco cultured cells.

Original languageEnglish
Pages (from-to)35-39
Number of pages5
JournalBioscience, Biotechnology and Biochemistry
Volume63
Issue number1
DOIs
Publication statusPublished - Jan 1 1999

Keywords

  • BY2 cell
  • N-glycans
  • Plant glycoprotein
  • Sugar chain
  • Tobacco cultured cell

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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