Structures of N-linked oligosaccharides of glycoproteins from tobacco BY2 suspension cultured cells

Nirianne Q. Palacpac, Yoshinobu Kimura, Kazuhito Fujiyama, Toshiomi Yoshida, Tatsuji Seki

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The structures of N-linked sugar chains of glycoproteins expressed in tobacco BY2 cultured cells are reported. Five pyridylaminated (PA-) N-linked sugar chains were derived and purified from hydrazinolysates of the glycoproteins by reversed-phase HPLC and size-fractionation HPLC. The structures of the PA-sugar chains purified were identified by two-dimensional PA-sugar chain mapping, ion-spray MS/MS analysis, and exoglycosidase digestions. The five structures fell into two categories; the major class (92.5% as molar ratio) was a xylose containing-type (Man3Fuc1 Xyl1GlcNAc2 (41.0%), GlcNAc2Man3Fuc1Xyl1GlcNAc 2 (26.5%), GlcNAc1MaD3Fuc1Xyl1GlcNAc 2 (21.7%), Man3 Xyl1GlcNAc2 (3.3%)), and the minor class was a high-mannose type (Man5GlcNAc2 (7.5%)). This is the first report to show that α(1-→3) fucosylation of N-glycans does occur but β(1→4) galactosylation of the sugar chains does not in the tobacco cultured cells.

Original languageEnglish
Pages (from-to)35-39
Number of pages5
JournalBioscience, Biotechnology and Biochemistry
Volume63
Issue number1
Publication statusPublished - Jan 1999

Fingerprint

Oligosaccharides
Glycoproteins
Tobacco
oligosaccharides
Sugars
cultured cells
glycoproteins
Cultured Cells
Suspensions
tobacco
High Pressure Liquid Chromatography
Cells
sugars
Glycoside Hydrolases
Xylose
Mannose
Polysaccharides
Digestion
Ions
glycosidases

Keywords

  • BY2 cell
  • N-glycans
  • Plant glycoprotein
  • Sugar chain
  • Tobacco cultured cell

ASJC Scopus subject areas

  • Food Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Structures of N-linked oligosaccharides of glycoproteins from tobacco BY2 suspension cultured cells. / Palacpac, Nirianne Q.; Kimura, Yoshinobu; Fujiyama, Kazuhito; Yoshida, Toshiomi; Seki, Tatsuji.

In: Bioscience, Biotechnology and Biochemistry, Vol. 63, No. 1, 01.1999, p. 35-39.

Research output: Contribution to journalArticle

Palacpac, Nirianne Q. ; Kimura, Yoshinobu ; Fujiyama, Kazuhito ; Yoshida, Toshiomi ; Seki, Tatsuji. / Structures of N-linked oligosaccharides of glycoproteins from tobacco BY2 suspension cultured cells. In: Bioscience, Biotechnology and Biochemistry. 1999 ; Vol. 63, No. 1. pp. 35-39.
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