Structure of the catalytic, inorganic core of oxygen-evolving photosystem II at 1.9 resolution

Keisuke Kawakami, Yasufumi Umena, Nobuo Kamiya, Jian Ren Shen

Research output: Contribution to journalReview article

140 Citations (Scopus)

Abstract

The catalytic center for photosynthetic water-splitting consists of 4 Mn atoms and 1 Ca atom and is located near the lumenal surface of photosystem II. So far the structure of the Mn4Ca-cluster has been studied by a variety of techniques including X-ray spectroscopy and diffraction, and various structural models have been proposed. However, its exact structure is still unknown due to the limited resolution of crystal structures of PSII achieved so far, as well as possible radiation damages that might have occurred. Very recently, we have succeeded in solving the structure of photosystem II at 1.9 , which yielded a detailed picture of the Mn4CaO5-cluster for the first time. In the high resolution structure, the Mn4CaO 5-cluster is arranged in a distorted chair form, with a cubane-like structure formed by 3 Mn and 1 Ca, 4 oxygen atoms as the distorted base of the chair, and 1 Mn and 1 oxygen atom outside of the cubane as the back of the chair. In addition, four water molecules were associated with the cluster, among which, two are associated with the terminal Mn atom and two are associated with the Ca atom. Some of these water molecules may therefore serve as the substrates for water-splitting. The high resolution structure of the catalytic center provided a solid basis for elucidation of the mechanism of photosynthetic water splitting. We review here the structural features of the Mn 4CaO5-cluster analyzed at 1.9 resolution, and compare them with the structures reported previously.

Original languageEnglish
Pages (from-to)9-18
Number of pages10
JournalJournal of Photochemistry and Photobiology B: Biology
Volume104
Issue number1-2
DOIs
Publication statusPublished - Jul 2011

Keywords

  • Crystal structure
  • Membrane protein structure
  • Oxygen-evolving complex
  • Photosystem II
  • Water-oxidation

ASJC Scopus subject areas

  • Radiation
  • Radiological and Ultrasound Technology
  • Biophysics
  • Radiology Nuclear Medicine and imaging

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