Structure of Physarum polycephalum cytochrome b5 reductase at 1.56 Å resolution

Sangwoo Kim, Michihiro Suga, Kyoko Ogasahara, Terumi Ikegami, Yoshiko Minami, Toshitsugu Yubisui, Tomitake Tsukihara

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Physarum polycephalum cytochrome b5 reductase catalyzes the reduction of cytochrome b5 by NADH. The structure of P. polycephalum cytochrome b5 reductase was determined at a resolution of 1.56 Å. The molecular structure was compared with that of human cytochrome b5 reductase, which had previously been determined at 1.75 Å resolution [Bando et al. (2004), Acta Cryst. D60, 1929-1934]. The high-resolution structure revealed conformational differences between the two enzymes in the adenosine moiety of the FAD, the lid region and the linker region. The structural properties of both proteins were inspected in terms of hydrogen bonding, ion pairs, accessible surface area and cavity volume. The differences in these structural properties between the two proteins were consistent with estimates of their thermostabilities obtained from differential scanning calorimetry data.

Original languageEnglish
Pages (from-to)274-279
Number of pages6
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume63
Issue number4
DOIs
Publication statusPublished - 2007
Externally publishedYes

Fingerprint

Physarum polycephalum
Cytochrome Reductases
Cytochromes b5
cytochromes
Oxidoreductases
Structural properties
proteins
adenosines
Flavin-Adenine Dinucleotide
Differential Scanning Calorimetry
Hydrogen Bonding
Molecular Structure
NAD
Adenosine
Molecular structure
Protons
enzymes
Differential scanning calorimetry
Hydrogen bonds
Proteins

Keywords

  • Cytochrome b reductase
  • Physarum polycephalum

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Structural Biology
  • Genetics
  • Condensed Matter Physics
  • Medicine(all)

Cite this

Structure of Physarum polycephalum cytochrome b5 reductase at 1.56 Å resolution. / Kim, Sangwoo; Suga, Michihiro; Ogasahara, Kyoko; Ikegami, Terumi; Minami, Yoshiko; Yubisui, Toshitsugu; Tsukihara, Tomitake.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 63, No. 4, 2007, p. 274-279.

Research output: Contribution to journalArticle

Kim, Sangwoo ; Suga, Michihiro ; Ogasahara, Kyoko ; Ikegami, Terumi ; Minami, Yoshiko ; Yubisui, Toshitsugu ; Tsukihara, Tomitake. / Structure of Physarum polycephalum cytochrome b5 reductase at 1.56 Å resolution. In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications. 2007 ; Vol. 63, No. 4. pp. 274-279.
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