TY - JOUR
T1 - Structure of photosystem I-LHCI-LHCII from the green alga Chlamydomonas reinhardtii in State 2
AU - Huang, Zihui
AU - Shen, Liangliang
AU - Wang, Wenda
AU - Mao, Zhiyuan
AU - Yi, Xiaohan
AU - Kuang, Tingyun
AU - Shen, Jian Ren
AU - Zhang, Xing
AU - Han, Guangye
N1 - Funding Information:
We thank Shenghai Chang in the Center of Cryo-Electron Microscopy (CCEM), Zhejiang University for the technical assistance on cryo-EM data collection, C. Ma from the Protein facility, School of Medicine, Zhejiang University for providing this platform for sample purification, X. Meng in Center of Biomedical Analysis, Tsinghua University, for protein MS analysis, L. Yu from the Institute of Botany, CAS for assistance in model building, W. Tang and Y. Yin from the Institute of Botany, CAS for instrumental supports in sample preparation, fluorescence measurement and high-performance liquid chromatography analysis. The project was funded by the National Key R&D Program of China (2017YFA0503700, 2017YFA0504803, 2018YFA0507700, 2020YFA0907600, 2019YFA0906300), a Strategic Priority Research Program of CAS (XDB17000000, XDA27050402), the Chinese Academy of Sciences Key Research Program of Frontier Sciences (QYZDY-SSW-SMC003), the Fundamental Research Funds for the Central Universities (2018XZZX001-13), and Youth Innovation Promotion Association of CAS (2020081).
Publisher Copyright:
© 2021, The Author(s).
PY - 2021/12
Y1 - 2021/12
N2 - Photosystem I (PSI) and II (PSII) balance their light energy distribution absorbed by their light-harvesting complexes (LHCs) through state transition to maintain the maximum photosynthetic performance and to avoid photodamage. In state 2, a part of LHCII moves to PSI, forming a PSI-LHCI-LHCII supercomplex. The green alga Chlamydomonas reinhardtii exhibits state transition to a far larger extent than higher plants. Here we report the cryo-electron microscopy structure of a PSI-LHCI-LHCII supercomplex in state 2 from C. reinhardtii at 3.42 Å resolution. The result reveals that the PSI-LHCI-LHCII of C. reinhardtii binds two LHCII trimers in addition to ten LHCI subunits. The PSI core subunits PsaO and PsaH, which were missed or not well-resolved in previous Cr-PSI-LHCI structures, are observed. The present results reveal the organization and assembly of PSI core subunits, LHCI and LHCII, pigment arrangement, and possible pathways of energy transfer from peripheral antennae to the PSI core.
AB - Photosystem I (PSI) and II (PSII) balance their light energy distribution absorbed by their light-harvesting complexes (LHCs) through state transition to maintain the maximum photosynthetic performance and to avoid photodamage. In state 2, a part of LHCII moves to PSI, forming a PSI-LHCI-LHCII supercomplex. The green alga Chlamydomonas reinhardtii exhibits state transition to a far larger extent than higher plants. Here we report the cryo-electron microscopy structure of a PSI-LHCI-LHCII supercomplex in state 2 from C. reinhardtii at 3.42 Å resolution. The result reveals that the PSI-LHCI-LHCII of C. reinhardtii binds two LHCII trimers in addition to ten LHCI subunits. The PSI core subunits PsaO and PsaH, which were missed or not well-resolved in previous Cr-PSI-LHCI structures, are observed. The present results reveal the organization and assembly of PSI core subunits, LHCI and LHCII, pigment arrangement, and possible pathways of energy transfer from peripheral antennae to the PSI core.
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U2 - 10.1038/s41467-021-21362-6
DO - 10.1038/s41467-021-21362-6
M3 - Article
C2 - 33597543
AN - SCOPUS:85101010323
SN - 2041-1723
VL - 12
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 1100
ER -