Structure of cag pathogenicity island in Japanese Helicobacter pylori isolates

S. Maeda, H. Yoshida, T. Ikenoue, K. Ogura, F. Kanai, Nobuyuki Kato, Y. Shiratori, M. Omata

Research output: Contribution to journalArticle

147 Citations (Scopus)

Abstract

Background - cag pathogenicity island (PAI) is reported to be a major virulence factor of Helicobacter pylori. Aim - To characterise cagA and the cag PAI in Japanese Hpylori strains. Methods - H pylori isolates from Japanese patients were evaluated for CagA by immunoblot, for cagA transcription by northern blot, and for cagA and 13 other cag PAI genes by Southern blot. cagA negative strains from Western countries were also studied. Induction of interleukin-8 secretion from gastric epithelial cells was also investigated. Results - All Japanese strains retained cagA. Fifty nine of 63 (94%) strains had all the cag PAI genes. In the remaining four, cag PAI was partially deleted, lacking cagA transcripts and not producing CagA protein. Details of the PAI of these strains were checked; three lacked cagB to cagQ (cagI) and continuously cagS to cag13 (cagII), and the remaining one lacked cagB to cag8. Western cagA negative strains completely lacked cag PAI including cagA. Nucleotide sequence analysis in one strain in which the cag PAI was partially deleted showed that the partial deletion contained 25 kb of cag PAI and the cagA promoter. Interleukin-8 induction was lower with the cag PAI partial deletion strains than with the intact ones. All Japanese cag PAI deleted strains were derived from patients with non-ulcer dyspepsia, whereas 41 of 59 (70%) CagA-producing strains were from patients with peptic ulcers or gastric cancer (p

Original languageEnglish
Pages (from-to)336-341
Number of pages6
JournalGut
Volume44
Issue number3
Publication statusPublished - 1999
Externally publishedYes

Fingerprint

Genomic Islands
Helicobacter pylori
Interleukin-8
Dyspepsia
Pylorus
Virulence Factors
Southern Blotting
Peptic Ulcer
Northern Blotting
Genes
Stomach Neoplasms
Sequence Analysis
Stomach
Epithelial Cells

Keywords

  • Helicobacter pylori
  • Japanese
  • Pathogenicity island

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Maeda, S., Yoshida, H., Ikenoue, T., Ogura, K., Kanai, F., Kato, N., ... Omata, M. (1999). Structure of cag pathogenicity island in Japanese Helicobacter pylori isolates. Gut, 44(3), 336-341.

Structure of cag pathogenicity island in Japanese Helicobacter pylori isolates. / Maeda, S.; Yoshida, H.; Ikenoue, T.; Ogura, K.; Kanai, F.; Kato, Nobuyuki; Shiratori, Y.; Omata, M.

In: Gut, Vol. 44, No. 3, 1999, p. 336-341.

Research output: Contribution to journalArticle

Maeda, S, Yoshida, H, Ikenoue, T, Ogura, K, Kanai, F, Kato, N, Shiratori, Y & Omata, M 1999, 'Structure of cag pathogenicity island in Japanese Helicobacter pylori isolates', Gut, vol. 44, no. 3, pp. 336-341.
Maeda S, Yoshida H, Ikenoue T, Ogura K, Kanai F, Kato N et al. Structure of cag pathogenicity island in Japanese Helicobacter pylori isolates. Gut. 1999;44(3):336-341.
Maeda, S. ; Yoshida, H. ; Ikenoue, T. ; Ogura, K. ; Kanai, F. ; Kato, Nobuyuki ; Shiratori, Y. ; Omata, M. / Structure of cag pathogenicity island in Japanese Helicobacter pylori isolates. In: Gut. 1999 ; Vol. 44, No. 3. pp. 336-341.
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AU - Maeda, S.

AU - Yoshida, H.

AU - Ikenoue, T.

AU - Ogura, K.

AU - Kanai, F.

AU - Kato, Nobuyuki

AU - Shiratori, Y.

AU - Omata, M.

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N2 - Background - cag pathogenicity island (PAI) is reported to be a major virulence factor of Helicobacter pylori. Aim - To characterise cagA and the cag PAI in Japanese Hpylori strains. Methods - H pylori isolates from Japanese patients were evaluated for CagA by immunoblot, for cagA transcription by northern blot, and for cagA and 13 other cag PAI genes by Southern blot. cagA negative strains from Western countries were also studied. Induction of interleukin-8 secretion from gastric epithelial cells was also investigated. Results - All Japanese strains retained cagA. Fifty nine of 63 (94%) strains had all the cag PAI genes. In the remaining four, cag PAI was partially deleted, lacking cagA transcripts and not producing CagA protein. Details of the PAI of these strains were checked; three lacked cagB to cagQ (cagI) and continuously cagS to cag13 (cagII), and the remaining one lacked cagB to cag8. Western cagA negative strains completely lacked cag PAI including cagA. Nucleotide sequence analysis in one strain in which the cag PAI was partially deleted showed that the partial deletion contained 25 kb of cag PAI and the cagA promoter. Interleukin-8 induction was lower with the cag PAI partial deletion strains than with the intact ones. All Japanese cag PAI deleted strains were derived from patients with non-ulcer dyspepsia, whereas 41 of 59 (70%) CagA-producing strains were from patients with peptic ulcers or gastric cancer (p

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