Structure of archaerhodopsin-2 at 1.8Å resolution

Tsutomu Kouyama, Ryudo Fujii, Soun Kanada, Taichi Nakanishi, Siu-Kit Chan, Midori Murakami

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Archaerhodopsin-2 (aR2), the sole protein found in the claret membrane of Halorubrum sp. Aus-2, functions as a light-driven proton pump. In this study, structural analysis of aR2 was performed using a novel three-dimensional crystal prepared by the successive fusion of claret membranes. The crystal is made up of stacked membranes, in each of which aR2 trimers are arranged on a hexagonal lattice. This lattice structure resembles that found in the purple membrane of H. salinarum, except that lipid molecules trapped within the trimeric structure are not distributed with perfect threefold symmetry. Nonetheless, diffraction data at 1.8Å resolution provide accurate structural information about functionally important residues. It is shown that two glutamates in the proton-release channel form a paired structure that is maintained by a low-barrier hydrogen bond. Although the structure of the proton-release pathway is highly conserved among proton-pumping archaeal rhodopsins, aR2 possesses the following peculiar structural features: (i) the motional freedom of the tryptophan residue that makes contact with the C13 methyl group of retinal is restricted, affecting the formation/decay kinetics of the L state, and (ii) the N-terminal polypeptide folds into an Ω-loop, which may play a role in organizing the higher-order structure.

Original languageEnglish
Pages (from-to)2692-2701
Number of pages10
JournalActa Crystallographica Section D: Biological Crystallography
Volume70
Issue number10
DOIs
Publication statusPublished - Sep 30 2014
Externally publishedYes

Fingerprint

Protons
Halorubrum
Microbial Rhodopsins
Purple Membrane
Glutamates
Proton Pumps
Membrane Fusion
Membranes
Tryptophan
Hydrogen
Lipids
Light
Peptides
Proteins

Keywords

  • archaerhodopsin-2
  • light-driven proton pump

ASJC Scopus subject areas

  • Structural Biology

Cite this

Kouyama, T., Fujii, R., Kanada, S., Nakanishi, T., Chan, S-K., & Murakami, M. (2014). Structure of archaerhodopsin-2 at 1.8Å resolution. Acta Crystallographica Section D: Biological Crystallography, 70(10), 2692-2701. https://doi.org/10.1107/S1399004714017313

Structure of archaerhodopsin-2 at 1.8Å resolution. / Kouyama, Tsutomu; Fujii, Ryudo; Kanada, Soun; Nakanishi, Taichi; Chan, Siu-Kit; Murakami, Midori.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. 70, No. 10, 30.09.2014, p. 2692-2701.

Research output: Contribution to journalArticle

Kouyama, T, Fujii, R, Kanada, S, Nakanishi, T, Chan, S-K & Murakami, M 2014, 'Structure of archaerhodopsin-2 at 1.8Å resolution', Acta Crystallographica Section D: Biological Crystallography, vol. 70, no. 10, pp. 2692-2701. https://doi.org/10.1107/S1399004714017313
Kouyama, Tsutomu ; Fujii, Ryudo ; Kanada, Soun ; Nakanishi, Taichi ; Chan, Siu-Kit ; Murakami, Midori. / Structure of archaerhodopsin-2 at 1.8Å resolution. In: Acta Crystallographica Section D: Biological Crystallography. 2014 ; Vol. 70, No. 10. pp. 2692-2701.
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